| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,676,992 | C→T | 20.9% | H247H (CAC→CAT) | bioH → | carboxylesterase of pimeloyl‑CoA synthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,676,992 | 0 | C | T | 20.9% | 109.2 / 18.8 | 76 | H247H (CAC→CAT) | bioH | carboxylesterase of pimeloyl‑CoA synthesis |
| Reads supporting (aligned to +/- strand): ref base C (24/36); new base T (4/12); total (28/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
AGCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACTTTACTG > minE/2676924‑2677056 | aGCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCAc > 1:2854819/1‑69 (MQ=255)aGCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCAc > 1:111298/1‑69 (MQ=255)aGCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCAc > 1:171928/1‑69 (MQ=255)aGCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCAc > 1:1293724/1‑69 (MQ=255)aGCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCAc > 1:3069397/1‑69 (MQ=255)aGCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTT‑TCAcc > 1:2459099/1‑69 (MQ=255) gCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCAcc > 1:2633256/1‑69 (MQ=255) gCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCAcc > 1:150848/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:1909607/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:603157/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:3245858/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:1591542/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:2197439/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:2209787/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:1309270/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:1240760/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:2671951/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:2724358/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGc > 1:27285/1‑69 (MQ=255) tatCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTATTGTCACCTGCTGGTGGc > 1:243190/1‑69 (MQ=255) cGCCAAAGCGGCCCATGCGCCGTTTATTTCACATCCGGACGAGTTTTGTCATCTGCTGGTGGCGTTGa > 1:3301770/1‑68 (MQ=255) cGCCAAAGCGGCCCATGCGCCGTTTATTTCACATCCGGACGAGTTTTGTCATCTGCTGGTGGCGTTGa > 1:1124683/1‑68 (MQ=255) cGCCAAAGCGGCCCATGCGCCGTTTATTTCACATCCGGACGAGTTTTGTCATCTGCTGGTGGCGTTGa > 1:127340/1‑68 (MQ=255) cGCCAAAGCGGCCCATGCGCCGTTTATTTCACATCCGGACGAGTTTTGTCATCTGCTGGTGGCGTTGa > 1:1287555/1‑68 (MQ=255) cAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGc < 1:68522/68‑1 (MQ=255) cAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGc < 1:2638208/68‑1 (MQ=255) cAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGc < 1:2595085/68‑1 (MQ=255) cAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGc < 1:244959/68‑1 (MQ=255) cAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGc < 1:2265605/68‑1 (MQ=255) cAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGc < 1:1400880/68‑1 (MQ=255) aaaGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGc < 1:1382861/67‑1 (MQ=255) gCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGgtg < 1:2959093/69‑1 (MQ=255) gCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGgtg < 1:2972316/69‑1 (MQ=255) cccATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGgtgt < 1:504222/69‑1 (MQ=255) cccATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGgtgt < 1:2940908/69‑1 (MQ=255) cccATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGgtg < 1:2600090/68‑1 (MQ=255) ccATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGgtgt < 1:908029/68‑1 (MQ=255) cgcCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTgg > 1:3049258/1‑69 (MQ=255) cgcCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTgg > 1:2211661/1‑69 (MQ=255) cgcCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTgg > 1:1436247/1‑69 (MQ=255) cgcCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTgg > 1:2915997/1‑69 (MQ=255) cgcCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTgg > 1:2081866/1‑69 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:1051387/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:3083145/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa > 1:947912/1‑69 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa > 1:33401/1‑69 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:3339124/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:974259/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:817432/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:898535/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa > 1:3044876/1‑69 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa > 1:3177297/1‑69 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa > 1:2721289/1‑69 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:2785126/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa > 1:2499903/1‑69 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:2462634/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:1166783/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa < 1:1816715/69‑1 (MQ=255) tatttCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGa > 1:2436364/1‑69 (MQ=255) gTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACtt < 1:1052924/69‑1 (MQ=255) gTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACtt < 1:1517310/69‑1 (MQ=255) gTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACtt < 1:3350133/69‑1 (MQ=255) gTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACtt < 1:3346284/69‑1 (MQ=255) gTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACtt < 1:2188741/69‑1 (MQ=255) gTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACtt < 1:2355288/69‑1 (MQ=255) ttttGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACttt < 1:2459936/69‑1 (MQ=255) ttttGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACttt < 1:2451104/69‑1 (MQ=255) ttttGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACttt < 1:3418895/69‑1 (MQ=255) ttttGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACttt < 1:492399/69‑1 (MQ=255) ttttGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACttt < 1:1943419/69‑1 (MQ=255) ttttGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACttt < 1:575116/69‑1 (MQ=255) ttttGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACttt < 1:739866/69‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:2677622/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:1055586/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:821324/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:3053612/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:772408/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:1886000/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:1932601/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:481236/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:2259426/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:2009857/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:2042406/68‑1 (MQ=255) tCATCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTCGAAATGGCGGGACAGAACTTTACTg < 1:2096512/68‑1 (MQ=255) | AGCGAATCATATATCTTCGCCAAAGCGGCCCATGCGCCATTTATTTCGCATCCGGCCGAGTTTTGTCACCTGCTGGTGGCGTTGAAGCAGAGGGTGTAGGTGGCTTTTGAAATGGCGAGACAGAACTTTACTG > minE/2676924‑2677056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |