BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,405	C→T	47.4%	L175L (CTG→CTA)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,405	0	C	T	47.4%	15.7 / 120.2	137	L175L (CTG→CTA)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base C (44/28); new base T (37/28); total (81/56)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

Only 100 of 137 total aligned reads displayed.

TGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAG  >  minE/2677349‑2677464

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:248849/1‑69 (MQ=255)

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:903596/1‑69 (MQ=255)

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:238399/1‑69 (MQ=255)

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:3013786/1‑69 (MQ=255)

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:2180895/1‑69 (MQ=255)

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:2946979/1‑69 (MQ=255)

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:1863063/1‑69 (MQ=255)

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:698285/1‑69 (MQ=255)

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:1473212/1‑69 (MQ=255)

tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                 >  1:1492121/1‑69 (MQ=255)

       ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgc                                           >  1:1338118/1‑68 (MQ=255)

       ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgc                                           >  1:3305765/1‑68 (MQ=255)

       ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgc                                           >  1:1626119/1‑68 (MQ=255)

       ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgc                                           >  1:3218064/1‑68 (MQ=255)

       ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgc                                           >  1:115228/1‑68 (MQ=255)

       ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgc                                           >  1:2590951/1‑68 (MQ=255)

       ccAGCCACATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgc                                           >  1:437377/1‑68 (MQ=255)

         aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctgc                                        >  1:2400906/1‑69 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACTTCGGTGCTGCATGATTtc                                >  1:2894614/1‑68 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAttt                                 >  1:458928/1‑67 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtat                                 >  1:87928/1‑65 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                >  1:2929807/1‑68 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                >  1:1847212/1‑68 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                >  1:1913675/1‑68 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                >  1:2808484/1‑68 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                >  1:604471/1‑68 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                >  1:2656690/1‑68 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                >  1:2293099/1‑68 (MQ=255)

                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAAGTCGGTGCTGCATGAttt                                 >  1:1530156/1‑67 (MQ=255)

                    gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                             <  1:3249035/69‑1 (MQ=255)

                    gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                             <  1:254381/69‑1 (MQ=255)

                    gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                             <  1:810145/69‑1 (MQ=255)

                    gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                             <  1:350573/69‑1 (MQ=255)

                    gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                             <  1:894823/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:2441860/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:1002023/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:426336/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:3304608/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:3157837/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:3017558/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:3034652/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:313038/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:1074321/69‑1 (MQ=255)

                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:1739537/69‑1 (MQ=255)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:1799360/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:3147090/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:1091348/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:1150375/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:1333296/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:1710195/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:1717727/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:2925398/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:87925/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:1785153/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:2435453/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:847937/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:353506/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:2737590/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:2725282/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:2618146/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:2066101/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:611940/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:2141119/59‑1 (MQ=39)

                           tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                <  1:2450721/59‑1 (MQ=39)

                            gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGc                      >  1:775369/1‑68 (MQ=255)

                            gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGc                      >  1:2006125/1‑68 (MQ=255)

                               gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATg                   <  1:1939696/68‑1 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:497710/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:973428/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:990590/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:1974839/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:1013271/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:1023801/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:2877244/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:2798401/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:2778029/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:2501717/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:1126618/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:1631026/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:1768573/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCgg                                              >  1:2336088/1‑38 (MQ=255)

                                  tatGTTTTTGAATTAACTCCAGTAGCGCCATACGTCg                                               >  1:876068/1‑37 (MQ=255)

                                   atGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:2842623/60‑1 (MQ=255)

                                   atGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt              <  1:1931744/69‑1 (MQ=255)

                                   atGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt              <  1:850949/69‑1 (MQ=255)

                                   atGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt              <  1:342585/69‑1 (MQ=255)

                                    tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt              >  1:2994106/1‑68 (MQ=255)

                                           gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                >  1:913802/1‑43 (MQ=38)

                                           gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                >  1:1931065/1‑43 (MQ=38)

                                           gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                >  1:3184583/1‑43 (MQ=38)

                                           gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                >  1:780852/1‑43 (MQ=38)

                                           gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                >  1:470948/1‑43 (MQ=38)

                                           gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                >  1:2375800/1‑43 (MQ=38)

                                           gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                >  1:2838041/1‑43 (MQ=38)

                                           gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                >  1:2966591/1‑43 (MQ=38)

                                           gAATTAACTCCAGTAGAGCCATACGTCGGTGCAGCATGATTtc                                >  1:234442/1‑43 (MQ=37)

                                           gAATTAAATCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                >  1:2098434/1‑43 (MQ=37)

                                             aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                       <  1:1070289/50‑1 (MQ=255)

                                                aaCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg  >  1:2122566/1‑68 (MQ=255)

                                                aaCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg  >  1:2907450/1‑68 (MQ=255)

TGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAG  >  minE/2677349‑2677464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`