BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,424	T→A	35.3%	Q169L (CAG→CTG)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,424	0	T	A	35.3%	103.6 / 96.4	116	Q169L (CAG→CTG)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base T (37/38); new base A (13/28); total (50/66)
Fisher's exact test for biased strand distribution p-value = 7.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

Only 100 of 117 total aligned reads displayed.

AGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAA  >  minE/2677358‑2677484

aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctgc                                                            >  1:2400906/1‑69 (MQ=255)

         aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACTTCGGTGCTGCATGATTtc                                                    >  1:2894614/1‑68 (MQ=255)

         aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtat                                                     >  1:87928/1‑65 (MQ=255)

         aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                                    >  1:2293099/1‑68 (MQ=255)

         aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                                    >  1:3164692/1‑68 (MQ=255)

         aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                                    >  1:463798/1‑68 (MQ=255)

         aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                                    >  1:604471/1‑68 (MQ=255)

         aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                                    >  1:2122999/1‑68 (MQ=255)

         aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                                    >  1:1913675/1‑68 (MQ=255)

         aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                                                    >  1:1847212/1‑68 (MQ=255)

           gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                                                 <  1:254381/69‑1 (MQ=255)

           gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                                                 <  1:350573/69‑1 (MQ=255)

           gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                                                 <  1:810145/69‑1 (MQ=255)

           gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                                                 <  1:3249035/69‑1 (MQ=255)

           gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                                                 <  1:894823/69‑1 (MQ=255)

            tCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatc                                                 <  1:2419680/68‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:1074321/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:2441860/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:426336/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:2057100/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:1924963/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:1821102/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:1002023/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:3157837/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:313038/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:3034652/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:1739537/69‑1 (MQ=255)

                 ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:3304608/69‑1 (MQ=255)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:3001780/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:353506/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:2749220/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1101831/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:2737590/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:2725282/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:424933/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:2618146/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:3147090/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1091348/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:2450721/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:2925398/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1150375/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:87925/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:847937/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1710195/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1717727/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1785153/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1799360/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:891746/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1956003/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:2066101/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1333296/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:611940/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:1263272/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:2371166/59‑1 (MQ=39)

                  tGACGAATATGTTTTTGAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    <  1:2141119/59‑1 (MQ=39)

                   gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGc                                          >  1:775369/1‑68 (MQ=255)

                   gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGc                                          >  1:2006125/1‑68 (MQ=255)

                      gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATg                                       <  1:1939696/68‑1 (MQ=255)

                          atGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:2842623/60‑1 (MQ=255)

                          atGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                  <  1:1931744/69‑1 (MQ=255)

                          atGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                  <  1:342585/69‑1 (MQ=255)

                          atGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                  <  1:764195/69‑1 (MQ=255)

                           tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                  >  1:2994106/1‑68 (MQ=255)

                           tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                                  <  1:846568/68‑1 (MQ=255)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGAtt                                                      >  1:1534061/1‑41 (MQ=38)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:3362528/1‑43 (MQ=38)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:913802/1‑43 (MQ=38)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:3184583/1‑43 (MQ=38)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:470948/1‑43 (MQ=38)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:780852/1‑43 (MQ=38)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:2375800/1‑43 (MQ=38)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:2966591/1‑43 (MQ=38)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:2838041/1‑43 (MQ=38)

                                  gAATTAACTCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:2950237/1‑43 (MQ=38)

                                  gAATTAACTCCAGTAGAGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:234442/1‑43 (MQ=37)

                                  gAATTAAATCCAGTAGCGCCATACGTCGGTGCAGCATGATTtc                                                    >  1:2098434/1‑43 (MQ=37)

                                    aTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                                           <  1:1070289/50‑1 (MQ=255)

                                       aaCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                      >  1:2907450/1‑68 (MQ=255)

                                       aaCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAg                      >  1:2122566/1‑68 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatc                        >  1:2837297/1‑48 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatc                        >  1:2705662/1‑48 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatc                        >  1:2657248/1‑48 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatc                        >  1:1701315/1‑48 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatc                        >  1:1931346/1‑48 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatc                        >  1:1398436/1‑48 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatc                        >  1:1273298/1‑48 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                  >  1:1629862/1‑54 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                  >  1:2493953/1‑54 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                  >  1:639985/1‑54 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa                  >  1:2520688/1‑54 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaa   >  1:2398757/1‑69 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaa   >  1:430535/1‑69 (MQ=255)

                                                         cGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaa   >  1:2960427/1‑69 (MQ=255)

                                                           tCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaaa  <  1:2979520/68‑1 (MQ=255)

                                                           tCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaaa  <  1:46927/68‑1 (MQ=255)

                                                           tCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaaa  <  1:3337865/68‑1 (MQ=255)

                                                           tCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaaa  <  1:1360884/68‑1 (MQ=255)

                                                           tCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaaa  <  1:1302469/68‑1 (MQ=255)

                                                           tCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaaa  <  1:3279000/68‑1 (MQ=255)

                                                           tCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATaaa  <  1:3238460/68‑1 (MQ=255)

AGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATGCGGAGGCATAAA  >  minE/2677358‑2677484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`