| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,678,154 | C→T | 32.4% | R72H (CGC→CAC) | yhgG ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,678,154 | 0 | C | T | 32.4% | 33.7 / 18.8 | 43 | R72H (CGC→CAC) | yhgG | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (15/14); new base T (11/3); total (26/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGACGCATACCGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTCC > minE/2678089‑2678219 | gcatacgcatacCGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCa < 1:2986968/69‑1 (MQ=255) ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:1014108/1‑69 (MQ=255) ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:2247546/1‑69 (MQ=255) ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:516585/1‑69 (MQ=255) ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:174986/1‑69 (MQ=255) ccGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:2855182/1‑69 (MQ=255) ccGGGCTTTCAAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCtttt > 1:3267492/1‑69 (MQ=255) gATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCGTTCCGGGCAGCTTTTACa < 1:731708/69‑1 (MQ=255) gATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTAc < 1:496814/68‑1 (MQ=255) aTGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACa < 1:2750171/68‑1 (MQ=255) ggAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:2242415/1‑69 (MQ=255) ggAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:2646622/1‑69 (MQ=255) ggAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:1440479/1‑69 (MQ=255) ggAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:2903479/1‑69 (MQ=255) ggAGTAAGGTTAACGCAGCGCCAACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACt > 1:1667119/1‑69 (MQ=255) acaaGGTTAACGCAACGCCCACCATTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACa < 1:1748666/61‑1 (MQ=255) gTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCagaga < 1:1912111/69‑1 (MQ=255) cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:1982602/68‑1 (MQ=255) cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:1391221/68‑1 (MQ=255) cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:2533736/68‑1 (MQ=255) cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:2272794/68‑1 (MQ=255) cGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCa < 1:2458205/68‑1 (MQ=255) gCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAg < 1:2605822/68‑1 (MQ=255) gCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAg < 1:62915/68‑1 (MQ=255) gCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAg < 1:1146367/68‑1 (MQ=255) cAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAg < 1:934917/67‑1 (MQ=255) caccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACa < 1:1677965/44‑1 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:1648573/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:1012278/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:44399/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:1531479/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:3057468/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:2974815/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:1787666/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:2610973/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:2128505/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGttc > 1:2556088/1‑69 (MQ=255) ccatTCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCGGAAAGACAGCCGTCAGGt > 1:123333/1‑67 (MQ=255) cacTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCttt > 1:2750875/1‑37 (MQ=255) cacTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCttt > 1:2085270/1‑37 (MQ=255) cacTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCttt > 1:545883/1‑37 (MQ=255) cacTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCttt > 1:1323011/1‑37 (MQ=255) attCGTGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACa < 1:1469789/38‑1 (MQ=255) cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGttcttc > 1:1879144/1‑67 (MQ=255) cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGttcttc > 1:3233767/1‑67 (MQ=255) cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:2881261/1‑68 (MQ=255) cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:2349912/1‑68 (MQ=255) cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:1435469/1‑68 (MQ=255) cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:912507/1‑68 (MQ=255) cgcgCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTcc > 1:2144839/1‑68 (MQ=255) | GCAGACGCATACCGGGCTTTCCAAAACAGGCGATGGAGTAAGGTTAACGCAGCGCCCACCACTCGCGCAGACAGGCTTTTCCTTCCGGGCAGCTTTTACAACTGCCAGAGAGGCAGCCGTCAGGTTCTTCC > minE/2678089‑2678219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |