| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,678,500 | C→T | 24.6% | A730A (GCG→GCA) | feoB ← | fused ferrous iron transporter, protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,678,500 | 0 | C | T | 24.6% | 123.1 / 32.6 | 97 | A730A (GCG→GCA) | feoB | fused ferrous iron transporter, protein B |
| Reads supporting (aligned to +/- strand): ref base C (40/33); new base T (10/14); total (50/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGATATCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAATG > minE/2678436‑2678564 | cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:787601/1‑68 (MQ=255)cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:310191/1‑68 (MQ=255)cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:2017241/1‑68 (MQ=255)cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:3044112/1‑68 (MQ=255)cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:526199/1‑68 (MQ=255)cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:1353612/1‑68 (MQ=255)cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:2759899/1‑68 (MQ=255)cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:2091660/1‑68 (MQ=255)cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:1435405/1‑68 (MQ=255)cGACATCTACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACTGcc > 1:2857587/1‑68 (MQ=255) atatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAg < 1:3046060/68‑1 (MQ=255) atatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAg < 1:3234908/68‑1 (MQ=255) atatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAg < 1:592509/68‑1 (MQ=255) atatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAg < 1:1460038/68‑1 (MQ=255) atatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAg < 1:2178971/68‑1 (MQ=255) atatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAg < 1:765625/68‑1 (MQ=255) tatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAg < 1:425169/67‑1 (MQ=255) tatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGaa < 1:1387149/69‑1 (MQ=255) tatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGaa < 1:1728701/69‑1 (MQ=255) tatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGaa < 1:2031386/69‑1 (MQ=255) tatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGaa < 1:1855724/69‑1 (MQ=255) tatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGaa < 1:12264/69‑1 (MQ=255) tatCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGaa < 1:3325712/69‑1 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGaa > 1:2686028/1‑67 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:2772111/1‑69 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg < 1:2946656/69‑1 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg < 1:2277041/69‑1 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:2450124/1‑69 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg < 1:3358108/69‑1 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:397564/1‑69 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg < 1:1662097/69‑1 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg < 1:62209/69‑1 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg < 1:66266/69‑1 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:711813/1‑69 (MQ=255) tCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:712697/1‑69 (MQ=255) cACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:3431299/1‑67 (MQ=255) cACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:592668/1‑67 (MQ=255) cACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:606953/1‑67 (MQ=255) cACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:625449/1‑67 (MQ=255) cACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:1014907/1‑67 (MQ=255) cACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:2383130/1‑67 (MQ=255) cACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATg > 1:1001716/1‑67 (MQ=255) cGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAg < 1:2090669/59‑1 (MQ=255) tACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGc > 1:1444779/1‑69 (MQ=255) tACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGc > 1:2651750/1‑69 (MQ=255) tACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGc > 1:480383/1‑69 (MQ=255) tACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGc > 1:2820070/1‑69 (MQ=255) ggcgCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTg < 1:207627/69‑1 (MQ=255) ggcgCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTg < 1:45773/69‑1 (MQ=255) ggcgCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTg < 1:2016341/69‑1 (MQ=255) agcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTT‑GATGCt < 1:3204598/69‑1 (MQ=255) agcagACCGATAACCACGATGTTAAACAGGATAACCGCCAg < 1:765122/41‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:3337050/69‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:1364844/69‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:3341143/69‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:1859654/69‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:2035843/69‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:1762730/69‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:2181736/69‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:1613115/69‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:2619549/69‑1 (MQ=255) gcagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:1248869/69‑1 (MQ=255) cagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:1328906/68‑1 (MQ=255) cagACCGATAACCACGATGTTAAACAGGATAACTGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:2062881/68‑1 (MQ=255) ccGATAACCACGTTGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGAc > 1:3121245/1‑67 (MQ=255) ccGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAg > 1:1697641/1‑55 (MQ=255) ccGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGAc > 1:888508/1‑67 (MQ=255) ccGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGAc > 1:3424653/1‑67 (MQ=255) ccGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGAc > 1:35517/1‑67 (MQ=255) ccGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGAc > 1:489124/1‑67 (MQ=255) cACGATGTTAAACAGGATAACTGCCAGAATGCACAGCAGGCTGTAAGTTGGATGCt < 1:1220756/56‑1 (MQ=255) tGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCt < 1:187255/69‑1 (MQ=255) tGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCt < 1:183273/69‑1 (MQ=255) tGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCt < 1:400092/69‑1 (MQ=255) tGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCt < 1:467412/69‑1 (MQ=255) tAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCt > 1:841736/1‑66 (MQ=255) tAAACAGGAAAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCt > 1:826654/1‑66 (MQ=255) gATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACaa > 1:2152451/1‑69 (MQ=255) gATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACaa > 1:1890672/1‑69 (MQ=255) gATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACaa > 1:1478948/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGc > 1:690486/1‑53 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:2428344/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:808356/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:1145819/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:720247/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:135597/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:1439895/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:2901531/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:2787245/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:2627123/1‑69 (MQ=255) aTAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAAt > 1:2602629/1‑69 (MQ=255) tAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:3398017/39‑1 (MQ=255) tAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:3394218/39‑1 (MQ=255) tAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:2549553/39‑1 (MQ=255) tAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:938964/39‑1 (MQ=255) aaCCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCt < 1:1945186/38‑1 (MQ=255) aaCCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAATg > 1:2311263/1‑68 (MQ=255) | CGATATCCACCCGGCTACGCGCGCGGCGCAGCAGACCGATAACCACGATGTTAAACAGGATAACCGCCAGAATGCACACCAGGCTGTAAGTTGGATGCTGACTGTAGCTGGCGACCTGATAGAACAATG > minE/2678436‑2678564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |