| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,678,875 | T→C | 23.9% | A605A (GCA→GCG) | feoB ← | fused ferrous iron transporter, protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,678,875 | 0 | T | C | 23.9% | 92.9 / 23.7 | 75 | A605A (GCA→GCG) | feoB | fused ferrous iron transporter, protein B |
| Reads supporting (aligned to +/- strand): ref base T (31/26); new base C (15/3); total (46/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAC > minE/2678811‑2678937 | tgtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCg > 1:2505346/1‑69 (MQ=255)tgtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCg > 1:1631564/1‑69 (MQ=255) gtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCgg > 1:908493/1‑69 (MQ=255) gtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCgg > 1:61548/1‑69 (MQ=255) gtCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAAAAGCTCTTCACCGAGGTTAAATTCTGCCgg > 1:1608703/1‑69 (MQ=255) tttCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGAt > 1:829156/1‑68 (MQ=255) tttCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGAt > 1:1840923/1‑68 (MQ=255) tttCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGAt > 1:257038/1‑68 (MQ=255) aGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGaa < 1:2252989/68‑1 (MQ=255) aGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGaa < 1:775809/68‑1 (MQ=255) ctctGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAActct > 1:289461/1‑69 (MQ=255) tGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGcag < 1:3079173/55‑3 (MQ=255) tGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGcag < 1:3264546/55‑3 (MQ=255) tGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCg > 1:3417416/1‑55 (MQ=255) tGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCg > 1:656256/1‑55 (MQ=255) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:3015792/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2760117/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:248948/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:1271805/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2394555/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2380382/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2554774/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:3245218/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:81861/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:2879922/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:1443741/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:3298207/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:1915493/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACCGAGGTTAAATTCCGCCGGATTg > 1:179694/1‑49 (MQ=38) aTCTACCGCACTGAACAGCTCTTCACAGAGGTTAAATTACGCCGGATTg > 1:3351751/1‑49 (MQ=255) aCTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCgg < 1:1469646/68‑1 (MQ=255) aCTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCgg < 1:3021121/68‑1 (MQ=255) aCTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTGCTGCgg < 1:442721/68‑1 (MQ=255) gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGgtg > 1:2783528/1‑67 (MQ=255) gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGgtg > 1:1603186/1‑67 (MQ=255) gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:3187166/69‑1 (MQ=255) gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:2218099/69‑1 (MQ=255) gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:445059/69‑1 (MQ=255) gAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:991276/69‑1 (MQ=255) aCAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTa < 1:435153/67‑1 (MQ=255) aGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTagag < 1:2576217/68‑1 (MQ=255) ctctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGgt > 1:1425884/1‑69 (MQ=255) ctctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGgt > 1:2942041/1‑69 (MQ=255) ctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAata > 1:1724474/1‑47 (MQ=255) ctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAata > 1:1439063/1‑47 (MQ=255) ctTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAata > 1:2946998/1‑47 (MQ=255) gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1208058/69‑1 (MQ=255) gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1283044/69‑1 (MQ=255) gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:3011870/69‑1 (MQ=255) gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1261904/69‑1 (MQ=255) gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:2430793/69‑1 (MQ=255) gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:2254562/69‑1 (MQ=255) gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1521653/69‑1 (MQ=255) gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:1636737/69‑1 (MQ=255) gAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGt < 1:199324/69‑1 (MQ=255) gTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc < 1:954986/69‑1 (MQ=255) gTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc < 1:2272675/69‑1 (MQ=255) gTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGAGGTGTAGAGGGTGTTGAGCGTAcc < 1:2982356/69‑1 (MQ=255) ttAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCgg < 1:2877201/46‑1 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:1965891/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:1693724/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:3246726/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:511224/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:526842/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:1488287/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:2475332/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:1408624/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:2976213/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:2686618/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTAcc > 1:2807156/1‑66 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc > 1:3328463/1‑69 (MQ=255) aaaTTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc > 1:1501633/1‑69 (MQ=255) aaaTTCCGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc < 1:2207901/69‑1 (MQ=255) aaaTTCCGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc < 1:550128/69‑1 (MQ=255) aaaTTCCGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAc < 1:2555415/69‑1 (MQ=255) | TGTCTTTCAGGCTCTGCCAGGTTTCATCTATCGCACTGAACAGCTCTTCACCGAGGTTAAATTCTGCCGGATTGAACTCTTCGTCCTGAATATTTTCTGCGGTGTAGAGGGTGTTGAGCGTACCCAC > minE/2678811‑2678937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |