| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,679,577 | C→G | 29.5% | G371G (GGG→GGC) | feoB ← | fused ferrous iron transporter, protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,679,577 | 0 | C | G | 29.5% | 89.3 / 43.8 | 71 | G371G (GGG→GGC) | feoB | fused ferrous iron transporter, protein B |
| Reads supporting (aligned to +/- strand): ref base C (22/28); new base G (13/8); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TTCCCCGGCAAGCCCAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGT > minE/2679515‑2679645 | ttCCCCGGCAAGCCCAGCTCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAg < 1:3336996/69‑1 (MQ=255)ttCCCCGGCAAGCCCAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAg < 1:2406075/69‑1 (MQ=255)ttCCCCGGCAAGCCCAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAg < 1:2740413/69‑1 (MQ=255) aGCCCAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaagga < 1:1027282/69‑1 (MQ=255) aGCCCAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaagga < 1:2931036/69‑1 (MQ=255) aGCCCAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaagga < 1:1486362/69‑1 (MQ=255) aGCCCAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaagga < 1:1614472/69‑1 (MQ=255) cccAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaagg < 1:463966/66‑1 (MQ=255) ccAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaaggaagg < 1:2670718/69‑1 (MQ=255) ccAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaaggaagg < 1:2721548/69‑1 (MQ=255) ccAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaaggaagg < 1:673572/69‑1 (MQ=255) ccAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaaggaagg < 1:677182/69‑1 (MQ=255) ccAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaaggaagg < 1:502410/69‑1 (MQ=255) ccAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaaggaagg < 1:853374/69‑1 (MQ=255) aGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAc > 1:2484145/1‑69 (MQ=255) gACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCaaggaagg < 1:1152932/54‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGATAGGAACAGGTAcatc < 1:3387697/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:3298094/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:1060789/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:1130885/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:121741/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:2908836/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:1408680/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:1967375/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:1510210/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:2653602/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:302827/69‑1 (MQ=255) cGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTAcatc < 1:1660907/69‑1 (MQ=255) acaAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATc > 1:711699/1‑69 (MQ=255) acaAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATc > 1:2937850/1‑69 (MQ=255) acaAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATc > 1:261505/1‑69 (MQ=255) acaAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATc > 1:838782/1‑69 (MQ=255) cgccgcACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCgg < 1:42341/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:65359/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:551639/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:295408/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:415054/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:2611129/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:1702344/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:1867300/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:2186889/69‑1 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTg > 1:3274847/1‑54 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:709508/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1023979/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:2109449/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1963994/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:2389807/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:3072381/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:3076382/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1656895/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:3127087/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1588826/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1404514/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGCAACAGGTACATCATGCCGATCTGc > 1:3361197/1‑55 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAGCAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:3134076/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATg > 1:1953869/1‑42 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCa > 1:672854/1‑45 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCa > 1:2274723/1‑45 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCAcc > 1:991609/1‑57 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCAcc > 1:2167868/1‑57 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:2836649/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:271626/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:2452904/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:2265745/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:2260473/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:1935720/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:1100477/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:883486/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:922928/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:1039580/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCA‑‑GGCAGCACGgt > 1:2880284/1‑69 (MQ=255) cccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGgt < 1:31126/69‑1 (MQ=255) cccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGgt < 1:2933759/69‑1 (MQ=255) cccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGgt < 1:2294592/69‑1 (MQ=255) cccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGgt < 1:862521/69‑1 (MQ=255) | TTCCCCGGCAAGCCCAGCGCCTGCATCAGACGGTCCATCACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGT > minE/2679515‑2679645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |