| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,679,619 | A→G | 44.8% | I357I (ATT→ATC) | feoB ← | fused ferrous iron transporter, protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,679,619 | 0 | A | G | 44.8% | 13.7 / 70.8 | 87 | I357I (ATT→ATC) | feoB | fused ferrous iron transporter, protein B |
| Reads supporting (aligned to +/- strand): ref base A (37/11); new base G (13/26); total (50/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCACCCAGGCCCTGGGCGAGGAAGAT > minE/2679554‑2679681 | acaAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATc > 1:261505/1‑69 (MQ=255)acaAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATc > 1:838782/1‑69 (MQ=255)acaAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATc > 1:711699/1‑69 (MQ=255)acaAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATc > 1:2937850/1‑69 (MQ=255) cgccgcACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCgg < 1:42341/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:2186889/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:2611129/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:295408/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:65359/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:1867300/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:551639/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:415054/69‑1 (MQ=255) cACGCGCCATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCa < 1:1702344/69‑1 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTg > 1:3274847/1‑54 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1588826/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1656895/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:3127087/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:3076382/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:3072381/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1404514/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1023979/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:709508/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:2109449/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:1963994/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGc > 1:2389807/1‑55 (MQ=255) cATATAGCCGGAGTCCTCAAGGAAGGAGAGCAACAGGTACATCATGCCGATCTGc > 1:3361197/1‑55 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAGCAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:3134076/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCa > 1:2274723/1‑45 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCa > 1:672854/1‑45 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCAcc > 1:2167868/1‑57 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCAcc > 1:991609/1‑57 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:271626/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:2836649/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:883486/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:922928/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:2452904/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:2260473/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:1039580/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:1100477/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:1935720/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACg > 1:2265745/1‑69 (MQ=255) taCCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCA‑‑GGCAGCACGgt > 1:2880284/1‑69 (MQ=255) gccGGAGTCCTCAAGGAAGGTGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:1155594/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGATAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:1715950/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACATGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:689153/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:1409541/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:3333606/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:1555959/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:739573/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:184939/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:2875851/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:2744203/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:2836033/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:27260/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:2206973/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:2049378/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:1150759/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:1979968/67‑1 (MQ=255) gccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:2031972/67‑1 (MQ=255) cccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGgt < 1:862521/69‑1 (MQ=255) cccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGgt < 1:2933759/69‑1 (MQ=255) cccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGgt < 1:2294592/69‑1 (MQ=255) cccGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGgt < 1:31126/69‑1 (MQ=255) ggAGTCCTCAAGGAAGGAGAGTAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGtt < 1:1168983/69‑1 (MQ=255) ggAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCGATCTGCGGCACCAGTGGCAGCACgg < 1:497655/65‑1 (MQ=255) ggAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGtt < 1:379903/69‑1 (MQ=255) ggAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGtt < 1:122007/69‑1 (MQ=255) ggAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGtt < 1:1935462/69‑1 (MQ=255) ggAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGtt < 1:1857811/69‑1 (MQ=255) ggAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGtt < 1:2883650/69‑1 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCAc > 1:13485/1‑51 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATgccgcc > 1:1456250/1‑66 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:3229529/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:882160/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:2284138/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:1289965/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:587844/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:430117/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:1452639/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:1527547/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:2315749/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:195868/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:2489535/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:2397669/1‑67 (MQ=255) gaaggaGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCa > 1:2345224/1‑67 (MQ=255) gagGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCACCCAGGcc > 1:163913/1‑69 (MQ=255) gAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCACCCAGGCCCTg > 1:1975433/1‑69 (MQ=255) catGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCACCCAGGCCCTGGGCGAGGAAGAt > 1:680014/1‑69 (MQ=255) catGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCACCCAGGCCCTGGGCGAGGAAGAt > 1:937005/1‑69 (MQ=255) | ACAAACGCCGCACGCGCCATATACCCGGAGTCCTCAAGGAAGGAGAGGAACAGGTACATCATGCCAATCTGCGGCACCAGTGGCAGCACGGTGTTAATGCCGCCACCCAGGCCCTGGGCGAGGAAGAT > minE/2679554‑2679681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |