| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,687,910 | A→G | 50.6% | A123A (GCT→GCC) | pck ← | phosphoenolpyruvate carboxykinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,687,910 | 0 | A | G | 50.6% | ‑2.5 / 81.9 | 89 | A123A (GCT→GCC) | pck | phosphoenolpyruvate carboxykinase |
| Reads supporting (aligned to +/- strand): ref base A (26/18); new base G (35/10); total (61/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
CAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAGATG > minE/2687851‑2687969 | cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGAc > 1:2084214/1‑68 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGAc > 1:1354092/1‑68 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGAc > 1:194624/1‑68 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACa > 1:2724599/1‑69 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACa > 1:2314554/1‑69 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACa > 1:2014096/1‑69 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACa > 1:2992110/1‑69 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACa > 1:1851355/1‑69 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACa > 1:3084713/1‑69 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGATCGCACCACAGAAGGCGTCGACa > 1:2472255/1‑69 (MQ=255)cAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGCGTTCGCACCACAGAAGGCGTCGACa > 1:1285701/1‑69 (MQ=255) ccACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:1105676/69‑1 (MQ=255) ccACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:2366600/69‑1 (MQ=255) ccACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:3044843/69‑1 (MQ=255) ccACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:1699781/69‑1 (MQ=255) ccACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:747859/69‑1 (MQ=255) ccACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:402127/69‑1 (MQ=255) cACTTCGGTGATGAAACGTACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:1305835/68‑1 (MQ=255) cACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:2833296/68‑1 (MQ=255) cACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:1799286/68‑1 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:2204809/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:1224123/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:2099657/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:2142837/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:2171160/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:333354/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:2272800/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:2300394/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:1195437/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:784066/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:835692/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:2677557/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:2930888/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:1969144/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:667129/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:663092/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:3058742/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:1744665/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:1710054/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:3426915/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:3158857/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:1522298/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:1677033/1‑54 (MQ=255) gaAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAAc > 1:3347512/1‑54 (MQ=255) aaaaGACGAGTATCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGc < 1:2858909/68‑1 (MQ=255) aaaaGACGAGTATCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGc < 1:2405743/68‑1 (MQ=255) aaaaGACGAGTATCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGc < 1:1148251/68‑1 (MQ=255) aaaaGACGAGTATCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGc < 1:1000368/68‑1 (MQ=255) gACGAGTATCCGGGTTCGCACCACAGAAGGCGTCGACAACGa < 1:1152348/42‑1 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAgg > 1:2611764/1‑69 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAgg > 1:832812/1‑69 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAgg > 1:3355435/1‑69 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAgg > 1:1649637/1‑69 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAgg > 1:329757/1‑69 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAgg > 1:1773067/1‑69 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAgg > 1:1879521/1‑69 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAgg > 1:296733/1‑69 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAgg > 1:2917073/1‑69 (MQ=255) tCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCAGAAAGCTGCCTGGTCACCAgg > 1:365490/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:382985/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:63046/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:1889547/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:3421211/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:1391352/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:2318901/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:1641168/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:2310219/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:3291530/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:701146/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:2387735/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:853476/1‑69 (MQ=255) cGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGcc > 1:3039576/1‑69 (MQ=255) ttCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCt > 1:2992839/1‑66 (MQ=255) tCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCa < 1:1378157/69‑1 (MQ=255) tCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCa < 1:3089280/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTGACCAGGCCTTTCAg < 1:1486792/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:1474270/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:436172/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:367289/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:1433818/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:3418002/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:3230360/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:1708641/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:2639058/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:1974629/69‑1 (MQ=255) cGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAg < 1:904341/69‑1 (MQ=255) caccacAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAGa < 1:170797/68‑1 (MQ=255) accacAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAGAtg > 1:531601/1‑69 (MQ=255) accacAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAGAt > 1:254464/1‑68 (MQ=255) | CAGGCCACTTCGGTGATGAAACGGACGGAAAGACGAGTATCCGGGTTCGCACCACAGAAAGCGTCGACAACGAACAGACGTTTGCCGGAAAGCTGCCTGGTCACCAGGCCTTTCAGATG > minE/2687851‑2687969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |