| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,690,326 | C→T | 67.3% | A557V (GCG→GTG) | yhgE → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,690,326 | 0 | C | T | 67.3% | 52.9 / 36.1 | 71 | A557V (GCG→GTG) | yhgE | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (10/13); new base T (16/31); total (27/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGAAAGAAAAATTACTCACAGCTGCGAAGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAATCTGAATGAGTGACAAAGCGC > minE/2690267‑2690389 | aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:1743128/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:2591195/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:2666743/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:2438673/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:225336/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:2708315/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:2162083/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:1688312/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:3080951/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:3192143/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:387508/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:100227/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:1051600/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:86855/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:573825/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGa > 1:1180709/1‑69 (MQ=255)aaCGAAAGAAAAATTACTCATGGCTGCGGAGGATGGAAAACTGGGGACGAAGCCTAAAGAGTGGCGCGa > 1:2214219/1‑69 (MQ=255) aagaaaAATTACTCACAGCTGCGAAGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTg < 1:401242/69‑1 (MQ=255) aagaaaAATTACTCACAGCTGCGAAGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTg < 1:2673579/69‑1 (MQ=255) aagaaaAATTACTCACAGCTGCGAAGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTg < 1:2452524/69‑1 (MQ=255) aagaaaAATTACTCACAGCTGCGAAGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTg < 1:2167602/69‑1 (MQ=255) aagaaaAATTACTCACAGCTGCGAAGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTg < 1:1480468/69‑1 (MQ=255) aTTACTCACAGCTGCGAAGTATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGa > 1:2914708/1‑69 (MQ=255) gCGAAGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTg < 1:1642984/69‑1 (MQ=255) ggaGGTTGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2831672/66‑2 (MQ=255) ggaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2711532/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgt < 1:2025956/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2828724/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2716182/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2713874/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2831932/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:997544/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:637643/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2902185/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:929079/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:56888/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1919095/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2573788/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1631567/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1474194/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1394890/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1636641/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1685038/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1108597/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1769830/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1957731/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1102749/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:197442/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:203958/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2080184/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:2223768/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1057591/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:1023940/66‑2 (MQ=255) gaGGATGGAAAACTGGGGACGAAGCCTAAACTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:3113384/66‑2 (MQ=255) aaGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGa < 1:3119387/67‑1 (MQ=255) aaGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGa < 1:2480558/67‑1 (MQ=255) aaGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGa < 1:3266874/67‑1 (MQ=255) aaGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGa < 1:3435245/67‑1 (MQ=255) aaGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGa < 1:3080137/67‑1 (MQ=255) aaGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAAt > 1:951716/1‑69 (MQ=255) aaGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAAt > 1:1521398/1‑69 (MQ=255) aaGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAAt > 1:1240197/1‑69 (MQ=255) gAAAACTGGGGACGAAGCCTAAAGTGTGGCGCGATCTTACGGTGGATGGCGAAAGACTgg < 1:373792/60‑2 (MQ=255) gAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAAt > 1:2671237/1‑68 (MQ=255) aaaCTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAATCTg < 1:60526/69‑1 (MQ=255) aaaCTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAATCTg < 1:1568225/69‑1 (MQ=255) aaGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAATCTGAATGAGTGACAAAgcgc > 1:2946776/1‑67 (MQ=255) aaGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAATCTGAATGAGTGACAAAgcgc > 1:2849613/1‑67 (MQ=255) aaGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAATCTGAATGAGTGACAAAgcgc > 1:275756/1‑67 (MQ=255) aaGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAATCTGAATGAGTGACAAAgcgc > 1:3264741/1‑67 (MQ=255) aaGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAATCTGAATGAGTGACAAAgcgc > 1:3140667/1‑67 (MQ=255) | AACGAAAGAAAAATTACTCACAGCTGCGAAGGATGGAAAACTGGGGACGAAGCCTAAAGCGTGGCGCGATCTTGTAGTGGATGGTGAAAGGCTGAATGTGAATCTGAATGAGTGACAAAGCGC > minE/2690267‑2690389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |