| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,696,696 | C→T | 31.6% | Q510Q (CAG→CAA) | mrcA ← | fused penicillin‑binding protein 1a murein transglycosylase and murein transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,696,696 | 0 | C | T | 31.6% | 95.5 / 50.9 | 104 | Q510Q (CAG→CAA) | mrcA | fused penicillin‑binding protein 1a murein transglycosylase and murein transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (34/37); new base T (16/17); total (50/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GCGCGTACCATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATC > minE/2696631‑2696764 | gcgcGTACCATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGc < 1:10602/69‑1 (MQ=255)gcgcGTACCATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGc < 1:759774/69‑1 (MQ=255)gcgcGTACCATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGc < 1:3274381/69‑1 (MQ=255)gcgcGTACCATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGc < 1:642536/69‑1 (MQ=255)gcgcGTACCATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGc < 1:3329325/69‑1 (MQ=255)gcgcGTACCATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGc < 1:1283268/69‑1 (MQ=255) cATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGa > 1:2043758/1‑69 (MQ=255) cATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGa > 1:1320143/1‑69 (MQ=255) cATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGa > 1:464153/1‑69 (MQ=255) aTCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGa < 1:1677540/68‑1 (MQ=255) aTCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGa < 1:2314778/68‑1 (MQ=255) aTCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGa < 1:624870/68‑1 (MQ=255) aTCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGa < 1:885888/68‑1 (MQ=255) aTCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGa < 1:1022318/68‑1 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:653696/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:2939019/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:956835/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:1962345/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:1862687/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:1780084/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:1032837/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:1033436/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:1270127/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGttc > 1:148810/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGtt > 1:284918/1‑68 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGtt > 1:738884/1‑68 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGtt > 1:1610244/1‑68 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGttc > 1:871955/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGttc > 1:2386963/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGttc > 1:2549437/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGttc > 1:2572140/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGttc > 1:1440318/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGttc > 1:1273780/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGttc > 1:1600370/1‑69 (MQ=255) accacGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGAACGGCATATTGCGGTGGTGAGttc > 1:1707903/1‑69 (MQ=255) gACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCa < 1:651599/68‑1 (MQ=255) gtGACCCAGCGCCTGACGTAAGCGAATTGGACCAGCATACTGc < 1:148984/42‑1 (MQ=255) tGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGt > 1:1713489/1‑52 (MQ=255) tGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCt > 1:3129449/1‑61 (MQ=255) tGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTc > 1:3350454/1‑68 (MQ=255) tGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTc > 1:3271788/1‑68 (MQ=255) gACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAg < 1:408389/69‑1 (MQ=255) aCCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGc < 1:1075196/40‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:649411/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:781585/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:467922/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:2735250/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:2959156/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:285271/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:1160910/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:1623943/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:184962/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:1712051/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:2285935/69‑1 (MQ=255) ccAGCCCCTGACGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:1393789/69‑1 (MQ=255) cAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGc < 1:2664369/37‑1 (MQ=255) gACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTg > 1:425310/1‑69 (MQ=255) gACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTg > 1:1962084/1‑69 (MQ=255) gACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTg > 1:1438410/1‑69 (MQ=255) aCGTAAGCGAATTGGACCGGCATATTGCGGTCGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:1895685/59‑1 (MQ=255) cGTAAGCGAATTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:2948878/58‑1 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAg > 1:1839586/1‑51 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAg > 1:1215833/1‑51 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAg > 1:2062776/1‑51 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAg > 1:90834/1‑51 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAg > 1:387500/1‑51 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAg > 1:196335/1‑51 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAg > 1:207290/1‑51 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAg > 1:274783/1‑51 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATccc > 1:1444408/1‑69 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATccc > 1:3336147/1‑69 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATccc > 1:902801/1‑69 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATccc > 1:2230526/1‑69 (MQ=255) aGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATcc > 1:2911393/1‑68 (MQ=255) aGCGAATTGGACCAGCATACCGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATccc > 1:314609/1‑69 (MQ=255) aTTGGACCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:34653/49‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:249415/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:2637162/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:2690803/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:26990/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:1442447/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:1892986/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:2215335/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:2955146/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:1287756/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:1316703/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:2958366/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:2414963/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:3003605/69‑1 (MQ=255) ggACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:2998976/69‑1 (MQ=255) gACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGaa < 1:1486307/68‑1 (MQ=255) aCCGGCATATTGCGGTGGTGAGTTCTTCGGTTGCCAGTCAGAAc < 1:1762111/44‑1 (MQ=38) cTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATc > 1:1566586/1‑69 (MQ=255) cTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATc > 1:760742/1‑69 (MQ=255) cTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATc > 1:543449/1‑69 (MQ=255) cTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATc > 1:42357/1‑69 (MQ=255) cTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATc > 1:103479/1‑69 (MQ=255) cTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATc > 1:1379660/1‑69 (MQ=255) cTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATc > 1:1880947/1‑69 (MQ=255) cTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATc > 1:950620/1‑69 (MQ=255) | GCGCGTACCATCACCACGTTTTTCGACTGACCCAGCCCCTGACGTAAGCGAATTGGACCAGCATACTGCGGTGGTGAGTTCTTCGGCTGCCAGTCAGAACCGGCACTTGCATCCCAGCGAGAAATTGGCACATC > minE/2696631‑2696764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |