| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,697,440 | C→T | 35.5% | P262P (CCG→CCA) | mrcA ← | fused penicillin‑binding protein 1a murein transglycosylase and murein transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,697,440 | 0 | C | T | 35.5% | 29.0 / 23.0 | 45 | P262P (CCG→CCA) | mrcA | fused penicillin‑binding protein 1a murein transglycosylase and murein transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (20/9); new base T (6/10); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01 | |||||||||||
ACCGTCTTCATAGGCACTTTCGCCATAACGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTGATCG > minE/2697374‑2697506 | aCCGTCTTCATAGGCACTTTCGCCATAACGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACgg > 1:821746/1‑69 (MQ=255) cACTTTCGCCATAACGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCa < 1:2336871/68‑1 (MQ=255) aCTTTCGCCATAACGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCATAGAAAGCAAt > 1:1131414/1‑69 (MQ=255) aCTTTCGCCATAACGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAAt > 1:3024178/1‑69 (MQ=255) aCTTTCGCCATAACGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAAt > 1:2310543/1‑69 (MQ=255) aaCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGt < 1:1164663/69‑1 (MQ=255) aaCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGt < 1:2051465/69‑1 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCag > 1:2971202/1‑47 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCa > 1:1043457/1‑55 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGt > 1:2794529/1‑68 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGt > 1:75966/1‑68 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGt > 1:3409289/1‑68 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGt > 1:2395817/1‑68 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTg < 1:1056502/69‑1 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTg < 1:2406834/69‑1 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTg < 1:2402660/69‑1 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTg < 1:2891539/69‑1 (MQ=255) aCGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTg < 1:1955877/69‑1 (MQ=255) aCGGTTATACATCTCATGGCGCACAATTTCGCTCAGGTACGGCGCAGAGAAAGCaa > 1:1727320/1‑56 (MQ=255) gTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATa < 1:2634306/69‑1 (MQ=255) ataCATCTCCTGGCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGtt > 1:926125/1‑69 (MQ=255) ataCATCTCCTGGCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGtt > 1:991127/1‑69 (MQ=255) ataCATCTCCTGGCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGtt > 1:2739004/1‑69 (MQ=255) ataCATCTCCTGGCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGtt > 1:1243432/1‑69 (MQ=255) ataCATCTCCTGGCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGtt > 1:2413633/1‑69 (MQ=255) ataCATCTCCTGGCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGtt > 1:2122312/1‑69 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:247004/68‑1 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:1489378/68‑1 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:1747853/68‑1 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:2393485/68‑1 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:2923474/68‑1 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:2140013/68‑1 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:3015040/68‑1 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:1793823/68‑1 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:3235904/68‑1 (MQ=255) ggCGCACCATTTCACTCAGGTATGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGGTAATc < 1:1825824/68‑1 (MQ=255) cAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTg > 1:2521129/1‑69 (MQ=255) cAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTg > 1:532265/1‑69 (MQ=255) cAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTg > 1:2762204/1‑69 (MQ=255) cAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTg > 1:2476704/1‑69 (MQ=255) tACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCa > 1:498299/1‑54 (MQ=255) tACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTGATCg > 1:3181443/1‑69 (MQ=255) tACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTGATCg > 1:310340/1‑69 (MQ=255) tACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTGATCg > 1:848410/1‑69 (MQ=255) tACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTGATCg > 1:2426338/1‑69 (MQ=255) | ACCGTCTTCATAGGCACTTTCGCCATAACGGTTATACATCTCCTGGCGCACCATTTCGCTCAGGTACGGCGCAGAGAAAGCAATCTCCGGCGCGTGATAGTTAGCGTTAATCGCCTCAGTGCGTGTCTGATCG > minE/2697374‑2697506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |