| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,698,806 | G→T | 41.5% | V154V (GTG→GTT) | yrfD → | predicted pilus assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,698,806 | 0 | G | T | 41.5% | 50.3 / 66.1 | 77 | V154V (GTG→GTT) | yrfD | predicted pilus assembly protein |
| Reads supporting (aligned to +/- strand): ref base G (22/23); new base T (7/25); total (29/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGATTCCTGCCTTTTTTACCTTCTCAT > minE/2698739‑2698866 | tGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCAtgtg > 1:1597271/1‑68 (MQ=255)tGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGa > 1:2475998/1‑69 (MQ=255)tGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGa > 1:3152931/1‑69 (MQ=255)tGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGa > 1:2059996/1‑69 (MQ=255)tGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGa > 1:1281139/1‑69 (MQ=255)tGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGa > 1:1327906/1‑69 (MQ=255)tGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGa > 1:1413222/1‑69 (MQ=255)tGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGa > 1:3030615/1‑69 (MQ=255) gCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGcgcg < 1:3052061/69‑1 (MQ=255) gCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGcgcg < 1:3151902/69‑1 (MQ=255) gCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGcgcg < 1:3316003/69‑1 (MQ=255) gCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGcgcg < 1:2905928/69‑1 (MQ=255) gCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGcgcg < 1:456264/69‑1 (MQ=255) gCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGcgcg < 1:613099/69‑1 (MQ=255) gCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGcgcg < 1:2354012/69‑1 (MQ=255) gcgcAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGAt < 1:282267/68‑1 (MQ=255) gcgcAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGAt < 1:2201354/68‑1 (MQ=255) gcgcAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGAt < 1:1272676/68‑1 (MQ=255) gcgcAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGAt < 1:1146864/68‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCTCGATCACTCCg < 1:3433841/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:359550/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:2740688/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:839300/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:1034304/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:261942/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:800606/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:2552989/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:76523/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:2553253/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:97070/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:1556520/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:1553854/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:2048863/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:1218242/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:891560/69‑1 (MQ=255) agcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:1704821/69‑1 (MQ=255) gcaaagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCg < 1:2500028/68‑1 (MQ=255) aagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCGGAt > 1:1848281/1‑68 (MQ=255) aagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCGGAt > 1:1458107/1‑68 (MQ=255) aagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCGGAt > 1:164999/1‑68 (MQ=255) aagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCGGAt > 1:1702695/1‑68 (MQ=255) aagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCGGAt > 1:332945/1‑68 (MQ=255) aagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCGGAt > 1:666019/1‑68 (MQ=255) aagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTTAGCGCGATCACTCCGGAt > 1:2242033/1‑68 (MQ=255) aagAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGAt > 1:1726318/1‑68 (MQ=255) tGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTg < 1:1431580/69‑1 (MQ=255) tGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTg < 1:2492286/69‑1 (MQ=255) tGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTg < 1:3125911/69‑1 (MQ=255) tGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTg < 1:3348213/69‑1 (MQ=255) tACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGAt < 1:2889955/68‑1 (MQ=255) tACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGAt < 1:514176/68‑1 (MQ=255) tACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGAt < 1:2894060/68‑1 (MQ=255) tACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGAt < 1:2572969/68‑1 (MQ=255) aaGGTTTCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGATTCCTGCCtttttt > 1:437527/1‑69 (MQ=255) aaGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCg > 1:3214137/1‑54 (MQ=255) aaGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGATTCCTGCCtttttt > 1:2199150/1‑69 (MQ=255) aaGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGATTCCTGCCtttttt > 1:45330/1‑69 (MQ=255) aaGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGATTCCTGCCttttt > 1:1482123/1‑68 (MQ=255) aaGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGATTCCTGCCttttt > 1:3013572/1‑68 (MQ=255) tGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGc > 1:785128/1‑40 (MQ=255) tGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGc > 1:1081980/1‑40 (MQ=255) tGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGc > 1:629000/1‑40 (MQ=255) tGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGc > 1:997776/1‑40 (MQ=255) tGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGc > 1:1155996/1‑40 (MQ=255) tGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGc > 1:2648238/1‑40 (MQ=255) tGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGc > 1:2154832/1‑40 (MQ=255) ggCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGc > 1:3144612/2‑40 (MQ=255) tgtTCATGTTAGCGCGATCACTCCGGATGCCAGTGCATTACAGCGATTCCTGCCTTTTTTACCTTCtca < 1:738026/69‑1 (MQ=255) tgtTCATGTTAGCGCGATCACTCCGGATGCCAGTGCATTACAGCGATTCCTGCCTTTTTTACCTTCtca < 1:2163700/69‑1 (MQ=255) ttCATGTTAGCGCGATCACTCCGGATGCCAGTGCATTTCAGCGATTCCTGCCTTTTTTACCTTCtcat < 1:3428871/68‑1 (MQ=255) ttCATGTTAGCGCGATCACTCCGGATGCCAGTGCATTACAGCGATTCCTGCCTTTTTTACCTTCtcat < 1:2673897/68‑1 (MQ=255) ttCATGTTAGCGCGATCACTCCGGATGCCAGTGCATTACAGCGATTCCTGCCTTTTTTACCTTCtcat < 1:2003802/68‑1 (MQ=255) ttCATGTTAGCGCGATCACTCCGGATGCCAGTGCATTACAGCGATTCCTGCCTTTTTTACCTTCtcat < 1:1042199/68‑1 (MQ=255) ttCATGTTAGCGCGATCACTCCGGATGCCAGTGCATTACAGCGATTCCTGCCTTTTTTACCCTCtcat < 1:434865/68‑1 (MQ=255) cATGTGAGCGCGATCACCCCGGATGGCAGTGCATTACAGCGAt < 1:2723869/43‑1 (MQ=255) cATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGAt < 1:517946/43‑1 (MQ=255) cATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGAt < 1:800973/43‑1 (MQ=255) cATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGAt < 1:165558/43‑1 (MQ=255) | TGACTGCCGCGCAAAGCAAAGAGCTGGCAACGCTGCTTACTCTGGCAGAAAGGTTGCGTGTTCATGTGAGCGCGATCACCCCGGATGCCAGTGCATTACAGCGATTCCTGCCTTTTTTACCTTCTCAT > minE/2698739‑2698866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |