| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,699,330 | G→A | 100% | K69K (AAG→AAA) | yrfC → | predicted fimbrial assembly protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,699,330 | 0 | G | A | 78.7% | 34.2 / 4.2 | 28 | K69K (AAG→AAA) | yrfC | predicted fimbrial assembly protein |
| Reads supporting (aligned to +/- strand): ref base G (1/5); new base A (0/22); total (1/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCATAGACGCAGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAGCCACGTTTGCTGGAGCAACAGCAGTTACGCGAACAGCGTTCGCAACGGCAGCGCCAGCGACAA > minE/2699267‑2699393 | acgcgTAAACGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACg < 1:52725/68‑1 (MQ=255)acgcgTAAACGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACg < 1:401013/68‑1 (MQ=255)acgcgTAAACGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACg < 1:1363984/68‑1 (MQ=255)acgcgTAAACGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACg < 1:150539/68‑1 (MQ=255)acgcgTAAACGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACg < 1:2974977/68‑1 (MQ=255)acgcgTAAACGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACg < 1:1888591/68‑1 (MQ=255)acgcgTAAACGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACg < 1:1996006/68‑1 (MQ=255)acgcgTAAACGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACg < 1:2006716/68‑1 (MQ=255)acgcgTAAACGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACg < 1:2411036/68‑1 (MQ=255) gcATAGACGCAGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAGCCACGtt < 1:1161276/69‑1 (MQ=255) gcATAGACGCAGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAGCCACGtt < 1:3083764/69‑1 (MQ=255) cGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAAGGAAACCACGTTTGCTgg < 1:2924982/68‑1 (MQ=255) cGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:2466982/68‑1 (MQ=255) cGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:16240/68‑1 (MQ=255) cGCCGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:1348465/68‑1 (MQ=255) cGCCGTTTTGCTTAAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:3073090/68‑1 (MQ=255) cGCCGTTTTGCTTAAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:3360324/68‑1 (MQ=255) cGCCGTTTTGCTTAAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:3368341/68‑1 (MQ=255) cGCCGTTTTGCTTAAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:1219149/68‑1 (MQ=255) cGCCGTTTTGCTTAAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:677923/68‑1 (MQ=255) cGCCGTTTTGCTTAAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:730466/68‑1 (MQ=255) ccgTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:3128123/64‑1 (MQ=255) tttGCTTAAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:1064212/62‑1 (MQ=255) ttGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAGCCACGTTTGCTGGAGCAAc > 1:3273990/1‑67 (MQ=255) tCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAGCCACGTTTGCTGGAGCAACAGCAGt < 1:2439133/68‑1 (MQ=255) tCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAGCCACGTTTGCTGGAGCAACAGCAGt < 1:147608/68‑1 (MQ=255) ggAACAACAACTCGCCCGCAGCTTACAGATAACGAAACCACGTTTGCTgg < 1:334380/50‑1 (MQ=255) aCGAAGCCACGTTTGCTGGAGCAACAGCAGTTACGCGAACATCGTTCGCAACGGCAGCGCCAGCGACaa < 1:2295984/69‑1 (MQ=255) | GCGCATAGACGCAGTTTTGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAGCCACGTTTGCTGGAGCAACAGCAGTTACGCGAACAGCGTTCGCAACGGCAGCGCCAGCGACAA > minE/2699267‑2699393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |