| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,700,761 | C→T | 27.6% | T123T (ACC→ACT) | hofQ → | predicted fimbrial transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,700,761 | 0 | C | T | 27.6% | 70.6 / 24.1 | 65 | T123T (ACC→ACT) | hofQ | predicted fimbrial transporter |
| Reads supporting (aligned to +/- strand): ref base C (26/21); new base T (12/6); total (38/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
CGCCAGGAGGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAG > minE/2700699‑2700826 | cgccAGGAGGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCaa < 1:2561824/69‑1 (MQ=255)cgccAGGAGGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCaa < 1:952244/69‑1 (MQ=255)cgccAGGAGGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCaa < 1:2373819/69‑1 (MQ=255)cgccAGGAGGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCaa < 1:2837307/69‑1 (MQ=255)cgccAGGAGGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCaa > 1:2911970/1‑69 (MQ=255) gccAGGAGGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCaa < 1:489280/68‑1 (MQ=255) gCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCg > 1:1282041/1‑69 (MQ=255) gCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCg > 1:1994876/1‑69 (MQ=255) cAAATCTGCCGCTGGTAAATCGCAGTATAACCCTGCAATACGcc > 1:3038090/1‑44 (MQ=255) cAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGcc > 1:458254/1‑44 (MQ=255) cAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGcc > 1:536871/1‑44 (MQ=255) cAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGcc > 1:2104429/1‑44 (MQ=255) cAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGcc > 1:1859171/1‑44 (MQ=255) cAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGcc > 1:1360949/1‑44 (MQ=255) cAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGc > 1:853098/1‑43 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:2738980/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:460513/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:264604/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:2642503/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:954930/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:952115/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:539940/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:2366843/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:1909212/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:215338/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:2099768/1‑66 (MQ=255) aTCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCgg > 1:1943000/1‑66 (MQ=255) gCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGc < 1:2297232/68‑1 (MQ=255) gCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGc < 1:611579/68‑1 (MQ=255) gCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGc < 1:1712715/68‑1 (MQ=255) gCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGc < 1:509261/68‑1 (MQ=255) gCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGc < 1:1749646/68‑1 (MQ=255) gCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGc < 1:677295/68‑1 (MQ=255) ggAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACt < 1:2148270/69‑1 (MQ=255) aaaTCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTg < 1:351391/67‑1 (MQ=255) aaaTCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTg < 1:2725159/67‑1 (MQ=255) tattACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCGGGGGAGaa < 1:1062280/51‑1 (MQ=37) tattACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCGGGGGAGaa < 1:229869/51‑1 (MQ=37) tattACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCGGGGGAGaa < 1:616095/51‑1 (MQ=37) tattACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCGGGGGAGaa < 1:825886/51‑1 (MQ=37) tattACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCGGGGGAGaa < 1:3204842/51‑1 (MQ=37) tattACTCTGCAATACGCCGACGCCGGAGAGCTGGCGAAAGCGGGGGAGaa < 1:967038/51‑1 (MQ=37) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGa > 1:2754609/1‑57 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:458485/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:870260/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:394649/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:2565841/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:2286667/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:1064896/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:1112295/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:1414822/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:1822943/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:2087989/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:2293210/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:2440034/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:2703990/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:2857918/1‑68 (MQ=255) taACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAggg > 1:3305846/1‑68 (MQ=255) aCCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAg < 1:2469009/68‑1 (MQ=255) aCCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAg < 1:3354765/68‑1 (MQ=255) aCCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAg < 1:2500563/68‑1 (MQ=255) aCCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAg < 1:1998475/68‑1 (MQ=255) aCCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAg < 1:2517737/68‑1 (MQ=255) aCCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAg < 1:1164431/68‑1 (MQ=255) aCCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAg < 1:1123861/68‑1 (MQ=255) | CGCCAGGAGGCGGAGCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGAAAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAG > minE/2700699‑2700826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |