| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,700,888 | G→A | 29.5% | A166T (GCG→ACG) | hofQ → | predicted fimbrial transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,700,888 | 0 | G | A | 29.5% | 95.3 / 41.8 | 95 | A166T (GCG→ACG) | hofQ | predicted fimbrial transporter |
| Reads supporting (aligned to +/- strand): ref base G (31/35); new base A (6/22); total (37/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT > minE/2700824‑2700952 | gAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAgcgcgc > 1:1006401/1‑68 (MQ=255)gAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAgcgcgc > 1:699625/1‑68 (MQ=255) aTGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTg > 1:2698819/1‑67 (MQ=255) aTGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTg > 1:1674724/1‑67 (MQ=255) aTGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTg > 1:2057856/1‑67 (MQ=255) aTGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTg > 1:3392705/1‑67 (MQ=255) aTGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTg > 1:2438660/1‑67 (MQ=255) tGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAAc < 1:245621/69‑1 (MQ=255) tGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAAc < 1:2000599/69‑1 (MQ=255) tGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAAc < 1:2629128/69‑1 (MQ=255) tGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAAc < 1:156352/69‑1 (MQ=255) tGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAAc < 1:354339/69‑1 (MQ=255) tGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAAc < 1:2226092/69‑1 (MQ=255) tGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAAc < 1:240072/69‑1 (MQ=255) tGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAAc < 1:2800914/69‑1 (MQ=255) aCCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAAc < 1:456585/67‑1 (MQ=255) aCCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTAGAACAg > 1:91811/1‑69 (MQ=255) aCCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTAGAACAg > 1:2564120/1‑69 (MQ=255) aCCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTAGAACAg > 1:1662412/1‑69 (MQ=255) aaCGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAgcg > 1:2644669/1‑68 (MQ=255) aaCGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAgcg > 1:1769290/1‑68 (MQ=255) aaCGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAgcg > 1:1264964/1‑68 (MQ=255) aaCGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAgcg > 1:2829894/1‑68 (MQ=255) cGCACCAATCGCCTTTTGCTACGAGATAACAAAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCa < 1:786411/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAACAAAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCa < 1:1393252/64‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCTCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:465953/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:958835/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:222361/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:908678/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:2471275/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:1333908/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:3003443/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:3125659/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:2888634/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:1871284/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:63953/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:2287720/69‑1 (MQ=255) cAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:2307828/69‑1 (MQ=255) cGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTTGGTAGCGCa < 1:2268296/59‑1 (MQ=255) cGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCa < 1:3431320/59‑1 (MQ=255) cGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCa < 1:865499/59‑1 (MQ=255) cGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCa < 1:1824147/59‑1 (MQ=255) cGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCa < 1:1669134/59‑1 (MQ=255) gCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGc > 1:2150913/1‑69 (MQ=255) gCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGc > 1:2147037/1‑69 (MQ=255) gCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGc > 1:2128239/1‑69 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGTGTATCGCAAATGGATCTGCCGGTCgg < 1:2447236/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGTGTATCGCAAATGGATCTGCCGGTCgg < 1:1661521/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:810776/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:3262287/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:1081550/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:3005422/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:1372492/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:2978163/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:1378146/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:2898164/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:1581047/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:2835528/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:2238419/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:2769379/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:269075/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:1807701/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:1939390/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:2627743/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:257306/65‑1 (MQ=255) ctgcGAGATAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:19860/65‑1 (MQ=255) agaTAACAAAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCa < 1:1743896/67‑1 (MQ=255) agaTAACAAAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCa < 1:2888497/67‑1 (MQ=255) agaTAACAAAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCa < 1:2892537/67‑1 (MQ=255) agaTAACAAAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCa < 1:2617337/67‑1 (MQ=255) agaTAAC‑AAACACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCa < 1:1275075/67‑1 (MQ=255) agaTAAC‑AAA‑ACGGCGTTAAGGACGCTTGAACAGTGGGTATCGCa > 1:3046740/1‑45 (MQ=255) agaTAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAg < 1:196613/67‑1 (MQ=255) agaTAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCa > 1:1037354/1‑45 (MQ=255) agaTAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCa > 1:1249524/1‑45 (MQ=255) agaTAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCa > 1:1161263/1‑45 (MQ=255) agaTAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCa > 1:1800256/1‑45 (MQ=255) agaTAAC‑AAA‑ACGGCGTTAAGCACGCTTGAACAGAGGGTat > 1:1992495/1‑40 (MQ=38) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTg > 1:3370788/1‑67 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:552349/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:1725884/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:731688/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:1826250/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:2030420/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:2656020/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:2948365/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:3392754/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:307552/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAg > 1:3331683/1‑69 (MQ=255) aaC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGCTCGGGCAGGTTGAg > 1:1496537/1‑69 (MQ=255) cGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:518800/52‑1 (MQ=255) tcGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGa < 1:1297810/37‑1 (MQ=255) tAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCgg < 1:1683602/46‑1 (MQ=255) aaGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCatat > 1:993389/1‑69 (MQ=255) aaGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCatat > 1:3244023/1‑69 (MQ=255) aaGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCatat > 1:76879/1‑69 (MQ=255) aaGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCatat > 1:580015/1‑69 (MQ=255) | GAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC‑AAA‑ACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT > minE/2700824‑2700952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |