| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,703,017 | T→C | 70.0% | D136D (GAT→GAC) | aroB → | 3‑dehydroquinate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,703,017 | 0 | T | C | 70.0% | 71.5 / 31.6 | 73 | D136D (GAT→GAC) | aroB | 3‑dehydroquinate synthase |
| Reads supporting (aligned to +/- strand): ref base T (7/15); new base C (44/7); total (51/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.25e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01 | |||||||||||
CGGCGGCGAGTTATCAGCGCGGTGTCCGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAACCATCCCCTCGGTAAAAACATGATTGGCGCGTTCT > minE/2702950‑2703081 | cggcggcgAGTTATCAGCGCGGTGTCCGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGAt < 1:837856/68‑1 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGTTCGActcctc > 1:1398791/1‑62 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:1963269/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:2657887/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:2614778/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:2592158/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:2830621/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:2869760/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:2424265/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:3182739/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:3248296/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:424673/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:1971842/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:1908209/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:991748/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:564133/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:904084/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:127697/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:891043/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:1359903/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:1368784/1‑63 (MQ=255) tATCAGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACtcctcc > 1:1806597/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:3276695/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:1083368/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:2896988/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:856908/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:800176/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:3379288/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:402361/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:75599/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:427111/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:506923/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:649999/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:2737730/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:561572/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:1443449/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:2518364/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:2481261/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:1723283/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:1780599/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:1858587/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:200453/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:2136352/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:2188028/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:2401122/1‑63 (MQ=255) aGCGCGGTGTACGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTg > 1:2191849/1‑63 (MQ=255) gtCCGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCg < 1:1299302/69‑1 (MQ=255) gtCCGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCg > 1:2753968/1‑69 (MQ=255) gtCCGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCg < 1:809807/69‑1 (MQ=255) tCCGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCg > 1:110386/1‑68 (MQ=255) tCCGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCg < 1:1646648/68‑1 (MQ=255) tttCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCa < 1:2851223/68‑1 (MQ=255) tttCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCa < 1:466942/68‑1 (MQ=255) tttCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCa < 1:512085/68‑1 (MQ=255) ttcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAc < 1:1189717/69‑1 (MQ=255) ttcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAc < 1:1501460/69‑1 (MQ=255) ttcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAc < 1:3230579/69‑1 (MQ=255) ttcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAc < 1:2113349/69‑1 (MQ=255) tcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAcc < 1:2405737/69‑1 (MQ=255) tcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAcc < 1:2625493/69‑1 (MQ=255) tcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAcc < 1:2346006/69‑1 (MQ=255) tcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAcc < 1:3209987/69‑1 (MQ=255) tcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAcc < 1:485455/69‑1 (MQ=255) tcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAcc < 1:3229149/69‑1 (MQ=255) tcattcaAGTCCCGACGACGTTACTGTCGCAGGTCGACTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAcc < 1:345789/69‑1 (MQ=255) cattcaAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGCAAAAACTGCGGTCAAc < 1:2704106/68‑1 (MQ=255) cgTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAAc < 1:947373/50‑1 (MQ=255) ttACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAACCATCCCCTCGGTAAAAACATg < 1:2548445/69‑1 (MQ=255) tACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAACCATCCCCTCGGTAAAAACATg < 1:577078/68‑1 (MQ=255) aGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCa < 1:2975427/36‑1 (MQ=255) gTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAACCATCCCCTCGGTAAAAACATGATTGGCGCGTTc > 1:2657442/1‑69 (MQ=255) tCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAACCATCCCCTCGGTAAAAAc > 1:1538983/1‑53 (MQ=255) cGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAACCATCCCCTCGGTAAAAACATGATTGGCGCGTTCt > 1:3094384/1‑68 (MQ=255) cGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAACCATCCCCTCGGTAAAAACATGATTGGCGCGTTCt > 1:3254970/1‑68 (MQ=255) cGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAACCATCCCCTCGGTAAAAACATGATTGGCGCGTTCt > 1:3345252/1‑68 (MQ=255) | CGGCGGCGAGTTATCAGCGCGGTGTCCGTTTCATTCAAGTCCCGACGACGTTACTGTCGCAGGTCGATTCCTCCGTTGGCGGC‑AAAACTGCGGTCAACCATCCCCTCGGTAAAAACATGATTGGCGCGTTCT > minE/2702950‑2703081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |