| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,705,113 | A→C | 25.5% | intergenic (+37/‑70) | damX → / → dam | hypothetical protein/DNA adenine methylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,705,113 | 0 | A | C | 25.5% | 65.2 / 28.2 | 55 | intergenic (+37/‑70) | damX/dam | hypothetical protein/DNA adenine methylase |
| Reads supporting (aligned to +/- strand): ref base A (31/10); new base C (7/7); total (38/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.83e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
ACAGGCCGATCTGAAGTAATCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAG > minE/2705058‑2705173 | aCAGGCCGATCTGAAGTAATCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAgcg > 1:116751/1‑69 (MQ=255)aCAGGCCGATCTGAAGTAATCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAgcg > 1:2510367/1‑69 (MQ=255)aCAGGCCGATCTGAAGTAATCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAgcg > 1:1950025/1‑69 (MQ=255)aCAGGCCGATCTGAAGTAATCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAgcg > 1:3067561/1‑69 (MQ=255) ggCCGATCTGAAGTAATCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTg < 1:2812767/69‑1 (MQ=255) ggCCGATCTGAAGTAATCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTg < 1:2816685/69‑1 (MQ=255) aTCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTg > 1:3165157/1‑54 (MQ=255) aTCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTg > 1:2869132/1‑54 (MQ=255) aTCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTg > 1:2090897/1‑54 (MQ=255) aTCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTg > 1:3432028/1‑54 (MQ=255) aTCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTg > 1:513256/1‑54 (MQ=255) aTCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTg > 1:554231/1‑54 (MQ=255) cAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCttt > 1:1388621/1‑69 (MQ=255) cAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCttt > 1:1285033/1‑69 (MQ=255) cAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCttt > 1:2343433/1‑69 (MQ=255) cAAGGTTATCTACCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGc > 1:248935/1‑66 (MQ=255) ctcCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGc > 1:1975114/1‑69 (MQ=255) ctcCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGc > 1:216631/1‑69 (MQ=255) ctcCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGc > 1:850555/1‑69 (MQ=255) gCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGgag < 1:174036/69‑1 (MQ=255) ggTTTATCATTGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTtctc < 1:3312246/53‑1 (MQ=39) ggTTTATCATTGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTtctc < 1:974739/53‑1 (MQ=39) ggTTTATCATTGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTtctc < 1:515939/53‑1 (MQ=39) ggTTTATCATTGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTtctc < 1:3037590/53‑1 (MQ=39) ggTTTATCATTGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTtctc < 1:524769/53‑1 (MQ=39) ggTTTATCATTGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTtctc < 1:2431657/53‑1 (MQ=39) ggTTTATCATTGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTtctc < 1:1740854/53‑1 (MQ=39) gTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGgt > 1:2197072/1‑68 (MQ=255) gTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGgt > 1:306898/1‑68 (MQ=255) cattGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta > 1:850803/3‑69 (MQ=255) cattGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta > 1:2188946/3‑69 (MQ=255) cattGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta > 1:627914/3‑69 (MQ=255) cattGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta > 1:1448647/3‑69 (MQ=255) cattGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta > 1:31081/3‑69 (MQ=255) cattGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta > 1:2451919/3‑69 (MQ=255) cattGCGGGCGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta > 1:3433967/3‑69 (MQ=255) gTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAttag < 1:127066/69‑1 (MQ=255) gTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAttag < 1:2530712/69‑1 (MQ=255) gTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAttag < 1:841816/69‑1 (MQ=255) gTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAttag < 1:2051578/69‑1 (MQ=255) gTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta < 1:2526739/68‑1 (MQ=255) gTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta < 1:1405624/68‑1 (MQ=255) gTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtta < 1:3344029/68‑1 (MQ=255) tGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAt > 1:1706073/1‑64 (MQ=255) tGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAt > 1:1453581/1‑64 (MQ=255) tGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAt > 1:2171772/1‑64 (MQ=255) tGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAt > 1:1390795/1‑64 (MQ=255) tGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAt > 1:2644892/1‑64 (MQ=255) tGCGGGAGTTGCCTGAAGAGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAt > 1:1438184/1‑64 (MQ=255) gCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGgttt > 1:2312674/1‑58 (MQ=255) gCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGgtgt > 1:1612343/1‑60 (MQ=255) gCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGgtgt > 1:3300094/1‑60 (MQ=255) gCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGgtgt > 1:3095741/1‑60 (MQ=255) gCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGgtgt > 1:1869838/1‑60 (MQ=255) gCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGgtgt > 1:2892246/1‑60 (MQ=255) | ACAGGCCGATCTGAAGTAATCAAGGTTATCTCCCGCAATGGTTTATCGTTGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAG > minE/2705058‑2705173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |