| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,712,432 | A→T | 68.4% | G643G (GGT→GGA) | nirB ← | nitrite reductase, large subunit, NAD(P)H‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,712,432 | 0 | A | T | 68.4% | 48.0 / 23.4 | 44 | G643G (GGT→GGA) | nirB | nitrite reductase, large subunit, NAD(P)H‑binding |
| Reads supporting (aligned to +/- strand): ref base A (9/5); new base T (17/13); total (26/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGCGGTTTTCCAGTTCCACGCCGAGGCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGACCGGTTTCGAA > minE/2712367‑2712488 | tAGCGGTTTTCCAGTTCCACGCCGAGGCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTAc < 1:696043/67‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:1041210/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:2851369/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:3136592/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:2718543/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:2637051/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:2062797/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:1927063/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:1783643/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:1159786/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:927182/69‑1 (MQ=255) gCCGAGGCCGACGCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:1181291/69‑1 (MQ=255) ccGTGGCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGc < 1:842875/69‑1 (MQ=255) ccGAGGCCGACTCTGTCGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:524899/68‑1 (MQ=255) cGAGGCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGc < 1:2877928/68‑1 (MQ=255) gCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGc > 1:1857981/1‑69 (MQ=255) gCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGc > 1:1023224/1‑69 (MQ=255) gCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGc > 1:2558784/1‑69 (MQ=255) gCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGc > 1:1083186/1‑69 (MQ=255) gCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGc > 1:1110409/1‑69 (MQ=255) cTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCa < 1:2657465/69‑1 (MQ=255) cGCCAACACCGTAGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACg < 1:1211559/52‑1 (MQ=255) gCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGc > 1:779345/1‑69 (MQ=255) gCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGc > 1:173782/1‑69 (MQ=255) gCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGc > 1:2645583/1‑69 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAg > 1:588425/1‑43 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:917848/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:643032/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:973185/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:751139/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:3041705/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:2920031/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:2788279/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:257304/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:2529029/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:2525093/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:2450441/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:2121171/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:1889221/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:167923/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAcc > 1:1407981/1‑64 (MQ=255) aGCGGCACCAGGTGCTTCCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGAc > 1:2355254/1‑63 (MQ=255) gCACCAGGTGCTACCCACGCAGGTTTTTGCCATACGCAGTGCTTTCGCATAGGCATGACCGGTTTCGaa > 1:395823/1‑69 (MQ=255) aGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCa < 1:1464023/44‑1 (MQ=255) | TAGCGGTTTTCCAGTTCCACGCCGAGGCCGACGCTGTCGCCAACGCCGTAGCGGCACCAGGTGCTACCCACGCAGGTTTTCGCCATACGCAGTGCTTTCGCATAGGCATGACCGGTTTCGAA > minE/2712367‑2712488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |