| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,713,305 | A→G | 39.8% | S352S (AGT→AGC) | nirB ← | nitrite reductase, large subunit, NAD(P)H‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,713,305 | 0 | A | G | 39.8% | 53.7 / 87.4 | 123 | S352S (AGT→AGC) | nirB | nitrite reductase, large subunit, NAD(P)H‑binding |
| Reads supporting (aligned to +/- strand): ref base A (40/34); new base G (16/33); total (56/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAT > minE/2713245‑2713364 | tACCGCACCTAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:3176591/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:2583422/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:334882/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:1473426/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:511530/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:1315535/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:706434/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:2941060/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:2884222/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:2413534/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcgtc < 1:2769/69‑1 (MQ=255)tACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtagtc < 1:1686914/69‑1 (MQ=255) gggTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGc < 1:2963509/69‑1 (MQ=255) gTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTa > 1:1914954/1‑69 (MQ=255) gTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTa > 1:1790279/1‑69 (MQ=255) gTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTa > 1:1681068/1‑69 (MQ=255) gTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTa > 1:3276538/1‑69 (MQ=255) gTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTa > 1:1644495/1‑69 (MQ=255) gTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTa > 1:1430882/1‑69 (MQ=255) gTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTa > 1:624657/1‑69 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCttt > 1:3106066/1‑36 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:2016789/1‑38 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:1040918/1‑38 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:928845/1‑38 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:1169520/1‑38 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:2148949/1‑38 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:83643/1‑38 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:711/1‑38 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:45835/1‑38 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:1926386/1‑38 (MQ=255) cGCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtc > 1:147544/1‑37 (MQ=255) agCTGACAATCAGGCGTTTGTAGATCTCTTTGCTTtcg > 1:3070737/2‑38 (MQ=38) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCt > 1:3033841/1‑45 (MQ=255) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCt > 1:3028233/1‑45 (MQ=255) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCt > 1:692783/1‑45 (MQ=255) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCt > 1:2077954/1‑45 (MQ=255) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCt > 1:680401/1‑45 (MQ=255) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCt > 1:2344028/1‑45 (MQ=255) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCt > 1:2794580/1‑45 (MQ=255) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCt > 1:1439514/1‑45 (MQ=255) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCt > 1:260918/1‑45 (MQ=255) cAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTg > 1:2896802/1‑69 (MQ=255) tttGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTTCGACCGTGCGCATc > 1:1164788/1‑69 (MQ=255) tttGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATc > 1:2723466/1‑69 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:2526046/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:369406/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:1459985/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:1398521/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:1594917/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:3238479/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:3216585/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:3211054/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:699787/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:3132907/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:1615433/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:1678918/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:2984178/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:2568312/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:2397076/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:2387467/46‑1 (MQ=255) gATCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:1231011/46‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCaa < 1:3405465/68‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:3036929/69‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:1961247/69‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:1234725/69‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:711634/69‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:3184891/69‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:246647/69‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:899306/69‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:411955/69‑1 (MQ=255) gATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:1418820/69‑1 (MQ=255) aTCTCTTTGCTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGc < 1:1320499/45‑1 (MQ=255) ctctTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:412434/66‑1 (MQ=255) ctctTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:43852/66‑1 (MQ=255) ctctTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:919553/66‑1 (MQ=255) ctctTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:1062221/66‑1 (MQ=255) ctctTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:2656034/66‑1 (MQ=255) ctctTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:2106459/66‑1 (MQ=255) ctctTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:2192490/66‑1 (MQ=255) ctctTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAt < 1:2034365/66‑1 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:684155/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:489183/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:986777/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:3434697/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:3432442/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:3430965/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:3393991/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:1583247/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:2481610/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:1597078/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:1013790/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:2911051/1‑59 (MQ=255) ttACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAc > 1:1963515/1‑58 (MQ=255) ttACTTTAGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCAcc > 1:2692671/1‑59 (MQ=255) tACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTgcg < 1:1232995/51‑1 (MQ=255) tACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTgcg < 1:805390/51‑1 (MQ=255) tACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTgcg < 1:2890043/51‑1 (MQ=255) tACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTgcg < 1:1522112/51‑1 (MQ=255) tACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTgcg < 1:3335240/51‑1 (MQ=255) tACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTgcg < 1:2602336/51‑1 (MQ=255) | TACCGCACCGAGCAGGGTTTTGTTGTCTTCGCTGACAATCAGGCGTTTGTAGATCTCTTTACTTTCGTCGAGGTAAACGTAGCTACGTGCGCCAGGCGTGCGACCGTGCGCATCACCAAT > minE/2713245‑2713364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |