| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,719,154 | C→T | 38.1% | L322L (CTG→TTG) | argD → | bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,719,154 | 0 | C | T | 38.1% | 78.1 / 85.3 | 131 | L322L (CTG→TTG) | argD | bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (36/45); new base T (9/41); total (45/86) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
ATCATCAATACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTGTTGATTG > minE/2719091‑2719217 | atcatcAATACCCCTGAAGTGCTTGAAGGTATTCAAGCGAAACGCCAGCATTTTGTTGACCATTTGCag < 1:635526/69‑1 (MQ=255)atcatcAATACCCCTGAAGTGCTGGAAGGTATTCAAGCGAAACGCCAGCATTTTGTTGACCATTTGCag < 1:1056102/69‑1 (MQ=255)atcatcAATACCCCTGAAGTGCTGGAAGGTATTCAAGCGAAACGCCAGCATTTTGTTGACCATTTGCag < 1:844153/69‑1 (MQ=255)atcatcAATACCCCTGAAGTGCTGGAAGGTATTCAAGCGAAACGCCAGCATTTTGTTGACCATTTGCag < 1:3132992/69‑1 (MQ=255)atcatcAATACCCCTGAAGTGCTGGAAGGTATTCAAGCGAAACGCCAGCATTTTGTTGACCATTTGCag < 1:1980376/69‑1 (MQ=255)atcatcAATACCCCTGAAGTGCTGGAAGGTATTCAAGCGAAACGCCAGCATTTTGTTGACCATTTGCag < 1:1169284/69‑1 (MQ=255)atcatcAATACCCCTGAAGTGCTGGAAGGTATTCAAGCGAAACGCCAGCATTTTGTTGACCATTTGCag < 1:336164/69‑1 (MQ=255) tcatcaATACCCCTGAAGTGCTGGAAGGTATTCAAGCGAAACGCCAGCATTTTGTTGACCATTTGCag < 1:2328166/68‑1 (MQ=255) atcaATACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGc > 1:938862/1‑64 (MQ=255) atcaATACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGc > 1:211352/1‑64 (MQ=255) atcaATACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGc > 1:3046688/1‑64 (MQ=255) atcaATACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGc > 1:3147091/1‑64 (MQ=255) atcaATACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGc > 1:61242/1‑64 (MQ=255) atcaATACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGc > 1:799295/1‑64 (MQ=255) atcaATACCCCTGAAGTGCTAGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGc > 1:1330144/1‑64 (MQ=255) atcaATACCCCTGAACTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGc > 1:2107795/1‑64 (MQ=255) tACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:496180/68‑1 (MQ=255) tACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:556821/68‑1 (MQ=255) tACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg > 1:893162/1‑68 (MQ=255) tACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg > 1:2193730/1‑68 (MQ=255) gTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTAc > 1:3161004/1‑69 (MQ=255) gTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTAc > 1:2550935/1‑69 (MQ=255) gTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTAc > 1:2818005/1‑69 (MQ=255) gTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTAc > 1:1160882/1‑69 (MQ=255) ggAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATg < 1:2443637/68‑1 (MQ=255) ggAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATg < 1:1716642/68‑1 (MQ=255) ggCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTAttt < 1:1258015/69‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:688628/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:2439931/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:550277/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:1181398/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:1219650/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:1112326/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:1351301/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:2630784/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:1932036/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:1109337/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:1297894/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:3088587/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:109015/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:919765/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:3021073/47‑1 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTa > 1:363336/1‑68 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTa > 1:2686671/1‑68 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTa > 1:1245543/1‑68 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTa > 1:3009700/1‑68 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTa > 1:585490/1‑68 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTa > 1:2224604/1‑68 (MQ=255) cATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTa > 1:1472509/1‑68 (MQ=255) aTTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:3315014/46‑1 (MQ=255) aTTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAgatcg < 1:1852475/46‑1 (MQ=255) cAGGCGAAACGCCATCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat < 1:1447415/69‑1 (MQ=255) cAGGCGAAACGCCAGTGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat > 1:3319883/1‑69 (MQ=255) cAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat < 1:3423900/69‑1 (MQ=255) cAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat < 1:824282/69‑1 (MQ=255) cAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat < 1:101748/69‑1 (MQ=255) cAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat < 1:32611/69‑1 (MQ=255) cAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat < 1:1313199/69‑1 (MQ=255) cAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat < 1:1871459/69‑1 (MQ=255) cAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat < 1:2865005/69‑1 (MQ=255) ggCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGata < 1:241361/68‑1 (MQ=255) gCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATAtt < 1:1610964/69‑1 (MQ=255) cGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGt < 1:18344/69‑1 (MQ=255) aGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGat < 1:1446402/56‑1 (MQ=255) ttgttgACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTg > 1:421032/1‑68 (MQ=255) ttgttgACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTg > 1:2994885/1‑68 (MQ=255) ttgttgACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTg > 1:1811646/1‑68 (MQ=255) ttgttgACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTg > 1:1861572/1‑68 (MQ=255) ttgttgACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTg > 1:2530055/1‑68 (MQ=255) ttgttgACCATCTGAAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTg > 1:3224629/1‑68 (MQ=255) cgagACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTGTTg < 1:2726330/66‑1 (MQ=255) cgagACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTGTTg < 1:2479535/66‑1 (MQ=255) atcATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg > 1:3281118/3‑69 (MQ=255) aCCATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg > 1:1337053/1‑69 (MQ=255) aCCATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg > 1:1049349/1‑69 (MQ=255) aCCATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg > 1:1550217/1‑69 (MQ=255) aCCATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg > 1:1207066/1‑69 (MQ=255) aCCATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg > 1:450210/1‑69 (MQ=255) aCCATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg > 1:365247/1‑69 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATTGGGCTGTTGATTg < 1:512294/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:1118733/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:1022636/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:1696448/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:179297/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:2748342/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:267962/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:703700/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:2603394/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:674604/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:228335/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:2379263/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:2296776/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:2303215/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:2309643/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:3264155/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:252489/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:437554/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:135702/68‑1 (MQ=255) ccATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:2379355/68‑1 (MQ=255) cATTTGCAGAAGATCGATCAGCAGTACGATGTGTTTAGCGATATTCGCGGTATGGGGCTGTTGATTg < 1:1719668/67‑1 (MQ=255) | ATCATCAATACCCCTGAAGTGCTGGAAGGCATTCAGGCGAAACGCCAGCGTTTTGTTGACCATCTGCAGAAGATCGATCAGCAGTACGATGTATTTAGCGATATTCGCGGTATGGGGCTGTTGATTG > minE/2719091‑2719217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |