| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,719,476 | C→T | 46.6% | intergenic (+65/+2) | argD → / ← yhfK | bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase/conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,719,476 | 0 | C | T | 46.6% | 5.4 / 49.1 | 60 | intergenic (+65/+2) | argD/yhfK | bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase/conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (18/14); new base T (14/14); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
AAATACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGACGC > minE/2719410‑2719538 | aaaTACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAt < 1:2124021/69‑1 (MQ=255) aaTACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAtt > 1:995093/1‑69 (MQ=255) aaTACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAtt > 1:956857/1‑69 (MQ=255) aaTACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAtt > 1:436325/1‑69 (MQ=255) aaTACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAtt > 1:14943/1‑69 (MQ=255) aaTACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAtt > 1:1895044/1‑69 (MQ=255) aaTACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAtt > 1:592141/1‑69 (MQ=255) aaTACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAt > 1:1891139/1‑68 (MQ=255) aaTACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAt > 1:1507715/1‑68 (MQ=255) cGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAt > 1:1346155/1‑64 (MQ=255) cGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAt > 1:391069/1‑64 (MQ=255) cGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAt > 1:1597324/1‑64 (MQ=255) cGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCAt > 1:543089/1‑64 (MQ=255) cAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAAt > 1:2280658/1‑69 (MQ=255) cAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAAt > 1:503592/1‑69 (MQ=255) cAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAAt > 1:1660576/1‑69 (MQ=255) aGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAAc > 1:2226074/1‑69 (MQ=255) gcgTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCa < 1:465613/69‑1 (MQ=255) gcgTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCa < 1:1703227/69‑1 (MQ=255) gcgTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCa < 1:1428306/69‑1 (MQ=255) gcgTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCa < 1:2505016/69‑1 (MQ=255) gcgTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCa < 1:3340632/69‑1 (MQ=255) aTGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:927141/1‑69 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAAc > 1:1996212/3‑48 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:2503911/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:2840314/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:2496438/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:3125490/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:319886/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:335810/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:2423788/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:722012/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:1061660/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:2371609/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:1599128/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:1779357/3‑68 (MQ=255) caccTCATCCGATGGTTTAGCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATccc > 1:2025678/3‑68 (MQ=255) ccTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTg > 1:754499/1‑69 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:418150/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:1237154/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:861120/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:1683064/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:1700462/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:1979245/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:2439489/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:3357937/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:2199613/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:3114204/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:2334010/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:1057280/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGCCgc < 1:2425424/68‑1 (MQ=255) gCCTGTATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTCATGCGGTCGCTGCCgc < 1:1651793/68‑1 (MQ=255) gCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGACgc < 1:200704/68‑1 (MQ=255) gCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGACgc < 1:2091785/68‑1 (MQ=255) gCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGACgc < 1:1774583/68‑1 (MQ=255) gCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGACgc < 1:2246526/68‑1 (MQ=255) gCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGACgc < 1:639609/68‑1 (MQ=255) gCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGACgc < 1:745838/68‑1 (MQ=255) gCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGACgc < 1:2782407/68‑1 (MQ=255) gCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGGCGCTGACgc < 1:2561054/68‑1 (MQ=255) | AAATACGAAGCATCAGGCAAAAGATGTCGGATGCGGCGTGAATGCCTTATCCGATGGTTTAGCCTGCATTACGCCTTCGAATCCCGCAACTTGCGACTCAGCCAAATCCCGTGATGCGGTCGCTGACGC > minE/2719410‑2719538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |