| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,720,333 | C→T | 31.8% | V416V (GTG→GTA) | yhfK ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,720,333 | 0 | C | T | 31.8% | 65.7 / 36.0 | 76 | V416V (GTG→GTA) | yhfK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (19/32); new base T (14/10); total (33/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGGAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGT > minE/2720265‑2720400 | tCGGGAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTc < 1:1007342/69‑1 (MQ=255) ggAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTcac < 1:152397/68‑1 (MQ=255) ggAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTcac < 1:1938975/68‑1 (MQ=255) ggAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTcac < 1:682640/68‑1 (MQ=255) ggAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTcac < 1:579447/68‑1 (MQ=255) ggAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTcac < 1:536093/68‑1 (MQ=255) ggAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTcac < 1:532156/68‑1 (MQ=255) ggAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTcac < 1:2934102/68‑1 (MQ=255) ggAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTcac < 1:3066527/68‑1 (MQ=255) gAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACaa < 1:2720621/69‑1 (MQ=255) ttttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATcc < 1:298140/69‑1 (MQ=255) ttttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATcc < 1:3040124/69‑1 (MQ=255) ttttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATcc < 1:3053524/69‑1 (MQ=255) ttttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATcc < 1:2187971/69‑1 (MQ=255) ttttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATcc < 1:3147892/69‑1 (MQ=255) ttttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATcc < 1:3197944/69‑1 (MQ=255) ttttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATcc < 1:3353869/69‑1 (MQ=255) ttttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATcc < 1:1331355/69‑1 (MQ=255) ttttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATcc < 1:751090/69‑1 (MQ=255) tttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCTCAATcc < 1:436153/68‑1 (MQ=255) ttAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATc > 1:445103/1‑66 (MQ=255) tGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACgg > 1:736617/1‑69 (MQ=255) tGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACgg > 1:1106028/1‑69 (MQ=255) tGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACg > 1:66427/1‑68 (MQ=255) cAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGt > 1:1339566/1‑69 (MQ=255) cAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGt > 1:2434551/1‑69 (MQ=255) cAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGt > 1:927143/1‑69 (MQ=255) ccACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTgcgc < 1:138613/69‑1 (MQ=255) ccACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTgcgc < 1:624733/69‑1 (MQ=255) ccGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGc < 1:175096/69‑1 (MQ=255) tGATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:341994/69‑1 (MQ=255) tGATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:113358/69‑1 (MQ=255) tGATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:2845538/69‑1 (MQ=255) tGATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:2621841/69‑1 (MQ=255) tGATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:1015983/69‑1 (MQ=255) tGATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:3293421/69‑1 (MQ=255) tGATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:146342/69‑1 (MQ=255) tGATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:2170290/69‑1 (MQ=255) tGATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:1610897/69‑1 (MQ=255) gATTAACCCGACCACGGTTCCCACGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCAtttt < 1:905708/68‑1 (MQ=255) gATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCAttttc > 1:744218/1‑68 (MQ=255) gATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtg > 1:2313264/1‑69 (MQ=255) gATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtg > 1:1013968/1‑69 (MQ=255) gATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtg > 1:3169996/1‑69 (MQ=255) gATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtg > 1:2141938/1‑69 (MQ=255) aTTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtgt > 1:3113722/1‑69 (MQ=255) aTTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtgt > 1:1636799/1‑69 (MQ=255) aTTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtgt > 1:305749/1‑69 (MQ=255) aTTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtgt > 1:3026424/1‑69 (MQ=255) aTTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtgt > 1:137918/1‑69 (MQ=255) aTTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtgt > 1:1234464/1‑69 (MQ=255) aTTAACCCGACCACGGTTCCCACGGAGAGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTtgt > 1:1773514/1‑69 (MQ=255) aCCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGc < 1:3146904/69‑1 (MQ=255) aCCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGc < 1:1755096/69‑1 (MQ=255) aCCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGc < 1:1284145/69‑1 (MQ=255) aCGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:2158577/69‑1 (MQ=255) ctgTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:900728/66‑1 (MQ=255) cccACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:700163/63‑1 (MQ=255) cccACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:462586/63‑1 (MQ=255) cccACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:3361961/63‑1 (MQ=255) cccACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:1009251/63‑1 (MQ=255) cccACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:1086815/63‑1 (MQ=255) cccACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:1355953/63‑1 (MQ=255) cccACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:226138/63‑1 (MQ=255) accACGGAGCGATTAACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAAt < 1:3286251/62‑1 (MQ=255) cGGAGCGATTTACAATCCTCAGCCGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:106501/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTgcgt > 1:2437737/1‑49 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:2461711/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:1509006/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:1600094/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:1850022/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:2098735/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:598974/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:2562830/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:523006/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:2971193/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:297383/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtaatc > 1:886134/1‑68 (MQ=255) cGGAGCGATTTACAATCCTCAGACGGGTTGCCCCATAGCCATTTTGCGTCAcc > 1:2774786/1‑53 (MQ=255) cGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGc < 1:982118/39‑1 (MQ=255) gAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAgg < 1:3316272/69‑1 (MQ=255) aGCGATTCACACTCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGa < 1:912907/69‑1 (MQ=255) aGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGa < 1:862371/69‑1 (MQ=255) aGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGa < 1:1371769/69‑1 (MQ=255) aGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGa < 1:2816951/69‑1 (MQ=255) cacaATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGt > 1:2047236/1‑68 (MQ=255) cacaATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGt > 1:3316049/1‑68 (MQ=255) | TCGGGAATTTTAAAGTGCAGCGCCACGCCCGCAATGATTAACCCGACCACGGTTCCCACGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGT > minE/2720265‑2720400 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |