| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,720,384 | C→T | 57.2% | T399T (ACG→ACA) | yhfK ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,720,384 | 0 | C | T | 57.2% | 23.9 / 70.5 | 91 | T399T (ACG→ACA) | yhfK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (32/7); new base T (27/25); total (59/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCAACGCTTAAC > minE/2720323‑2720450 | cGGAGCGATTTACAATCCTCAGCCGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:106501/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:1850022/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:523006/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:2461711/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:1600094/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:598974/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:1509006/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:297383/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:2971193/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:2562830/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtcatc > 1:2098735/1‑68 (MQ=255)cGGAGCGATTTACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGCGTCACCAGCAATACTGtaatc > 1:886134/1‑68 (MQ=255) gAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAgg < 1:3316272/69‑1 (MQ=255) aGCGATTCACACTCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGa < 1:912907/69‑1 (MQ=255) aGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGa < 1:1371769/69‑1 (MQ=255) aGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGa < 1:862371/69‑1 (MQ=255) aGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGa < 1:2816951/69‑1 (MQ=255) cacaATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGt > 1:2047236/1‑68 (MQ=255) cacaATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGt > 1:3316049/1‑68 (MQ=255) ccTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACtt > 1:167969/1‑69 (MQ=255) ccTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACtt > 1:3406609/1‑69 (MQ=255) ccTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACtt > 1:2604638/1‑69 (MQ=255) ccTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACt > 1:2055306/1‑68 (MQ=255) cGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGa > 1:615292/1‑69 (MQ=255) gCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGc > 1:531581/1‑52 (MQ=255) gCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCa > 1:1546551/1‑53 (MQ=255) gCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCa > 1:1401104/1‑53 (MQ=255) gCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCa > 1:1678155/1‑53 (MQ=255) aTTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGcac < 1:2961557/67‑3 (MQ=255) aTTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCAt < 1:2537246/69‑1 (MQ=255) ttGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAg > 1:2666117/1‑69 (MQ=255) ttGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAg > 1:163139/1‑69 (MQ=255) tCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTg > 1:2362532/1‑68 (MQ=255) tCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTg > 1:1266342/1‑68 (MQ=255) tCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTg > 1:101813/1‑68 (MQ=255) tCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTg > 1:1300432/1‑68 (MQ=255) tCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTg > 1:2999726/1‑68 (MQ=255) tCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTg > 1:1861462/1‑68 (MQ=255) tCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTg > 1:34572/1‑68 (MQ=255) tCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTg > 1:165541/1‑68 (MQ=255) aCCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAgc > 1:1083234/1‑48 (MQ=255) aCCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGc > 1:2072536/1‑68 (MQ=255) aCCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCa > 1:2305690/1‑69 (MQ=255) aCCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCa > 1:2415206/1‑69 (MQ=255) aCCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCa > 1:2629825/1‑69 (MQ=255) aCCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCa > 1:205312/1‑69 (MQ=255) aCCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCa > 1:2038795/1‑69 (MQ=255) aCCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCa > 1:1225798/1‑69 (MQ=255) aCCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCAACAGGCTGGCa > 1:2664843/1‑69 (MQ=255) aGCAATTCTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:439285/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGTCAACg > 1:2818111/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAAc > 1:2074186/1‑68 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:3040342/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:91146/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:976443/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:2260946/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:2082796/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:1841180/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:1700233/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:1580990/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:1560639/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:2129973/1‑69 (MQ=255) aGCAATACTGTCATCAGGATCCAGTAAGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:791387/1‑69 (MQ=255) aGCAATACTGTCATCAGGATACAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACg > 1:1920057/1‑69 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:1415707/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:1425729/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:811347/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:810226/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:1800525/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:1216817/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:510614/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:109856/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:1786750/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:1058988/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:3025712/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:2171239/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:2128356/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:2853626/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:285506/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:2097184/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:2092407/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:2481854/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:1894956/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:3180789/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:3237501/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:1846014/69‑1 (MQ=255) aaTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCACATCAGGCTGGCAACGCtt < 1:3297503/69‑1 (MQ=255) aTACTGTCATCAGGATCCAGTACGACTTCGGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:1698358/68‑1 (MQ=255) aTACTGTCATCAGGATCCAGTACGACTTCAGCAGATGAAGCGCGGTGCCCATCAGGCTGGCAACGCtt < 1:411023/68‑1 (MQ=255) aCCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCAACGCTTAAc > 1:1528162/1‑69 (MQ=255) aCCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCAACGCTTAAc > 1:27504/1‑69 (MQ=255) ccGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCAACGCTTAAc > 1:914115/1‑68 (MQ=255) | CGGAGCGATTCACAATCCTCAGACGGGTTGCGCCATAGCCATTTTGTGTCACCAGCAATACCGTCATCAGGATCCAGTACGACTTCGGCAGATGCAGCGCGGTGCCCATCAGGCTGGCAACGCTTAAC > minE/2720323‑2720450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |