| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,721,332 | T→C | 55.0% | L83L (CTA→CTG) ‡ | yhfK ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,721,332 | 0 | T | C | 55.0% | 8.5 / 38.6 | 51 | L83L (CTA→CTG) ‡ | yhfK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (20/3); new base C (14/14); total (34/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.28e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGCCAAGTACCAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGTT > minE/2721269‑2721399 | gACGCCAAGTACCAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCtg > 1:113147/1‑69 (MQ=255)gACGCCAAGTACCAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCtg > 1:240255/1‑69 (MQ=255)gACGCCAAGTACCAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCtg > 1:2224734/1‑69 (MQ=255)gACGCCAAGTACCAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCtg > 1:2137574/1‑69 (MQ=255) aCGCCAAGTACCAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCtgt < 1:1257077/69‑1 (MQ=255) tACCAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCa < 1:218316/68‑1 (MQ=255) ccAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGc > 1:1634803/1‑69 (MQ=255) ccAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGc > 1:263691/1‑69 (MQ=255) ccAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGc > 1:818271/1‑69 (MQ=255) ccAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGc > 1:2726655/1‑69 (MQ=255) ccAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGc > 1:3166770/1‑69 (MQ=255) ccAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGc > 1:3353646/1‑69 (MQ=255) ccAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGc > 1:1364474/1‑69 (MQ=255) ccAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGc > 1:931216/1‑69 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:3138316/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:2949960/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:2894284/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:2307544/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:3233153/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:2718088/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:612600/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:1041790/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:1894116/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:152775/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:164596/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:1846930/69‑1 (MQ=255) ttAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:1774813/69‑1 (MQ=255) aaTCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAgg < 1:2015292/67‑1 (MQ=255) cGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCa > 1:3374293/1‑68 (MQ=255) cGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCa > 1:910196/1‑68 (MQ=255) cGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCa > 1:1548599/1‑68 (MQ=255) cGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCa > 1:409884/1‑68 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCt > 1:350621/1‑51 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:565821/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:1387750/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:3174887/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:220833/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:2609987/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:1685294/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:3827/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:1662805/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:3389072/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:67188/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:155524/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:2894738/1‑69 (MQ=255) cAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGCACCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGa > 1:1218085/1‑69 (MQ=255) ggCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCa < 1:1901879/69‑1 (MQ=255) tCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGt > 1:692391/1‑69 (MQ=255) tCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGt > 1:912595/1‑69 (MQ=255) tCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGt > 1:521745/1‑69 (MQ=255) tCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGAGt > 1:255072/1‑69 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGAc > 1:260933/1‑39 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACg > 1:369306/1‑68 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACg > 1:982476/1‑68 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACg > 1:2927146/1‑68 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACg > 1:1314864/1‑68 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACg > 1:1506016/1‑68 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACg > 1:2275184/1‑68 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACg > 1:2518440/1‑68 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACg > 1:3136741/1‑68 (MQ=255) agtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACAAATGATTAAGCGTTTGAAAAAACg > 1:1289435/1‑68 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt > 1:257974/1‑69 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt > 1:2510601/1‑69 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt > 1:245895/1‑69 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt > 1:2659971/1‑69 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt > 1:1900487/1‑69 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt < 1:1650177/69‑1 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt > 1:875199/1‑69 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt > 1:272042/1‑69 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt > 1:1495820/1‑69 (MQ=255) gtagCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGtt > 1:947535/1‑69 (MQ=255) | GACGCCAAGTACCAGCGTTAATCCGGTCAGCAAAAAGGGCAGGGGAACATCTTTTGCCAGTAGTAGCTGTGTCAGCAAGCTACAGGTGGCAAACAGCGACGCACCAATGATTAAGCGTTTGAAAAAACGTT > minE/2721269‑2721399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |