| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,722,373 | A→G | 59.4% | intergenic (‑122/‑180) | crp ← / → yhfA | DNA‑binding transcriptional dual regulator/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,722,373 | 0 | A | G | 59.4% | 50.1 / 79.4 | 113 | intergenic (‑122/‑180) | crp/yhfA | DNA‑binding transcriptional dual regulator/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (14/32); new base G (45/22); total (59/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
TCTGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAG > minE/2722305‑2722431 | tctGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGa > 1:2810844/1‑69 (MQ=255)tctGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGa > 1:2850890/1‑69 (MQ=255)tctGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGa > 1:3374350/1‑69 (MQ=255)tctGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGa > 1:3378834/1‑69 (MQ=255)tctGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGa > 1:1394558/1‑69 (MQ=255)tctGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGa > 1:1445103/1‑69 (MQ=255) tGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACg > 1:444091/1‑69 (MQ=255) tGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACg > 1:3115376/1‑69 (MQ=255) tGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACg > 1:2472006/1‑69 (MQ=255) tGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACg > 1:327722/1‑69 (MQ=255) tGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACg > 1:885091/1‑69 (MQ=255) tGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACg > 1:148920/1‑69 (MQ=255) tGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACg > 1:410289/1‑69 (MQ=255) aaTATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATa < 1:1948199/69‑1 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATa > 1:3105587/1‑68 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:3337952/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:3364712/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:2369517/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:1966156/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:1153872/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:2047663/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:814515/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:3147659/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:2847791/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:159667/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:2746341/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:2749601/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATAc > 1:1432049/1‑69 (MQ=255) atatGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGGAGTCCTTTGCATAc > 1:2550905/1‑69 (MQ=255) tCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAg < 1:1848091/68‑1 (MQ=255) tCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAg < 1:841707/68‑1 (MQ=255) tCCCGCCACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAg < 1:644264/68‑1 (MQ=255) cTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATc < 1:359536/69‑1 (MQ=255) cTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATc < 1:2628775/69‑1 (MQ=255) cTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATc < 1:574750/69‑1 (MQ=255) cTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATc < 1:126798/69‑1 (MQ=255) cTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATc < 1:1793079/69‑1 (MQ=255) cTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATc < 1:3379051/69‑1 (MQ=255) aCCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCaa < 1:520617/69‑1 (MQ=255) aCCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCaa < 1:1124792/69‑1 (MQ=255) aCCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCaa < 1:1529683/69‑1 (MQ=255) ccTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCaa < 1:791565/68‑1 (MQ=255) ggAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATc < 1:1760037/62‑1 (MQ=255) aaGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTAtt < 1:1788556/68‑1 (MQ=255) aaGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTAtt < 1:3414451/68‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:399689/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:3713/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2987332/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:3397777/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:358576/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:251033/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1564220/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1663509/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2649550/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1948900/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2031030/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2056944/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2331643/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:935127/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2484715/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:710024/69‑1 (MQ=255) tCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATc < 1:344289/51‑1 (MQ=255) aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATa > 1:45340/1‑36 (MQ=255) aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATc > 1:3159764/1‑68 (MQ=255) aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATc > 1:2563186/1‑68 (MQ=255) aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATc > 1:1085225/1‑68 (MQ=255) cTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCt < 1:1197961/68‑1 (MQ=255) cTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCt < 1:2021927/68‑1 (MQ=255) cTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCt < 1:2831998/68‑1 (MQ=255) cTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCt < 1:2773581/68‑1 (MQ=255) gTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGAc > 1:2922184/1‑69 (MQ=255) gTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGAc > 1:3357231/1‑69 (MQ=255) cTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:202315/69‑1 (MQ=255) cTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:1598348/69‑1 (MQ=255) tGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:1323737/68‑1 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTa > 1:956484/1‑67 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTa > 1:1740807/1‑67 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTa > 1:2599275/1‑67 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:971212/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:748165/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2752711/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:606949/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:624306/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:630320/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3393633/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3179071/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3157599/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3092179/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2883145/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2786394/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2507093/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2332169/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2161062/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2157312/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1725968/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1560250/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:151340/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1321802/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1146762/1‑68 (MQ=255) aTGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:1093741/59‑1 (MQ=255) | TCTGGATTGCCGAAATATGCTTCCCGCTACCTGGGAAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAG > minE/2722305‑2722431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |