| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,722,403 | G→A | 65.9% | intergenic (‑152/‑150) | crp ← / → yhfA | DNA‑binding transcriptional dual regulator/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,722,403 | 0 | G | A | 65.9% | 82.8 / 65.6 | 114 | intergenic (‑152/‑150) | crp/yhfA | DNA‑binding transcriptional dual regulator/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (25/14); new base A (29/46); total (54/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 115 total aligned reads displayed.
AAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAG > minE/2722340‑2722470 | aaGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTAtt < 1:1789184/68‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2987332/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1663509/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1948900/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1982234/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2031030/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2056944/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2331643/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2484715/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:251033/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2649550/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:2517657/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:3397777/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:358576/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:3713/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:399689/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:551815/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:679161/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:710024/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:935127/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:986841/69‑1 (MQ=255) ggggCTATCAACTGTACTGCACGGTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACt < 1:1546612/69‑1 (MQ=255) aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATc > 1:2563186/1‑68 (MQ=255) aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATc > 1:3159764/1‑68 (MQ=255) aaCTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATc > 1:1085225/1‑68 (MQ=255) cTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCt < 1:2773581/68‑1 (MQ=255) cTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCt < 1:2831998/68‑1 (MQ=255) gTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGAc > 1:3357231/1‑69 (MQ=255) gTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGAc > 1:1711441/1‑69 (MQ=255) gTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGAc > 1:2922184/1‑69 (MQ=255) cTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:1598348/69‑1 (MQ=255) cTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:202315/69‑1 (MQ=255) tGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:1323737/68‑1 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTa > 1:956484/1‑67 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTa > 1:1740807/1‑67 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTa > 1:2599275/1‑67 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2883145/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2898545/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3157599/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2752711/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3179071/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3105634/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3092179/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:3393633/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2507093/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1416604/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1321802/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1725968/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:971212/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:748165/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1560250/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:630320/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:151340/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2110988/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:606949/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2157312/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:1146762/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2332169/1‑68 (MQ=255) ggTAATGTGGCGTCCTTTGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAg > 1:2459951/1‑68 (MQ=255) aTGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGt < 1:1093741/59‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:3292994/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:592761/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:3328509/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1211838/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:655104/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:3368618/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1225071/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1056569/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1726230/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1563100/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2699895/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2009771/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2575183/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1883501/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1015329/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2288717/69‑1 (MQ=255) ttGCATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1842161/69‑1 (MQ=255) ttGCATAAATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:2821145/69‑1 (MQ=255) cATACATGCAGTACATCAATATATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTtgt < 1:1862710/66‑1 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1520481/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1146626/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:886695/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:934011/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1057631/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:952160/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1615336/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:2136394/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:2240476/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:544161/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:1375842/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:3136168/1‑69 (MQ=255) aCATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTcc > 1:2991858/1‑69 (MQ=255) cATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGtctc < 1:2390147/67‑1 (MQ=255) tGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTgg > 1:227808/1‑69 (MQ=255) tGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTgg > 1:2757681/1‑69 (MQ=255) cAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGgtg > 1:885937/1‑69 (MQ=255) cAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGgtg > 1:2533752/1‑69 (MQ=255) tGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAg < 1:3078869/69‑1 (MQ=255) tGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAg < 1:1364334/69‑1 (MQ=255) gTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAg < 1:1481875/68‑1 (MQ=255) | AAGGGGCTATCAACTGTACTGCACGGTAATGTGACGTCCTTTGCATACATGCAGTACATCAATGTATTACTGTAGCATCCTGACTGTTTTAGCATAGCTTTCGCTTTGTGTCTCCTGGTGTCTCGCTTCAG > minE/2722340‑2722470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |