| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,723,207 | G→C | 43.7% | L225L (CTC→CTG) | prkB ← | predicted phosphoribulokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,723,207 | 0 | G | C | 43.7% | 40.7 / 120.6 | 112 | L225L (CTC→CTG) | prkB | predicted phosphoribulokinase |
| Reads supporting (aligned to +/- strand): ref base G (23/40); new base C (14/35); total (37/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 112 total aligned reads displayed.
CAGCCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCTGGAAGTTAA > minE/2723147‑2723274 | cagcCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:772318/69‑1 (MQ=255)cagcCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:1079620/69‑1 (MQ=255)cagcCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:2210596/69‑1 (MQ=255)cagcCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:1362923/69‑1 (MQ=255)cagcCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:437209/69‑1 (MQ=255)cagcCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:2253937/69‑1 (MQ=255)cagcCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:1319194/69‑1 (MQ=255)cagcCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:3269434/69‑1 (MQ=255)cagcCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:130729/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACTAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:2942277/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:572789/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:1028924/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:397302/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:670292/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:2632415/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:2367433/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:708513/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:2814662/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:2383713/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:720767/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:1317289/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:858134/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:98316/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:1136814/69‑1 (MQ=255) ccAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:1109340/69‑1 (MQ=255) cAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:3184596/68‑1 (MQ=255) ggAAATCGATCCCTTCCAGAATACCAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCtt > 1:120348/1‑69 (MQ=255) gAAATCGATCCCTTCCAGATTACGAAAATGGATCAGCACAAAGCTTTCATCGAGCGACGGGATGCCttt > 1:805472/1‑69 (MQ=255) gAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:3208383/60‑1 (MQ=255) gAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGAt < 1:2665853/63‑1 (MQ=255) aaaTCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:2537134/59‑1 (MQ=255) aaaTCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:44824/59‑1 (MQ=255) aaaTCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACgg < 1:733399/59‑1 (MQ=255) tCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:2143125/68‑1 (MQ=255) tCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:1944953/68‑1 (MQ=255) tCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:362751/68‑1 (MQ=255) tCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:465971/68‑1 (MQ=255) tCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:2575101/68‑1 (MQ=255) tCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:1691995/68‑1 (MQ=255) tCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:713330/68‑1 (MQ=255) tCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:1647037/68‑1 (MQ=255) tCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:2846750/68‑1 (MQ=255) cccTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:29300/67‑1 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGAc > 1:2553374/1‑46 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:2639362/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:290715/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:406409/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:2486892/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:2443627/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:3269858/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:216679/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:1976104/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:410404/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:1837215/1‑69 (MQ=255) ttCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCTTTTGCCGCGAACgg > 1:1070102/1‑69 (MQ=255) tCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACggg < 1:3343104/69‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGa < 1:2618427/69‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGa < 1:964147/69‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGa < 1:1957226/69‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGa < 1:2631252/69‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:2777296/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:987572/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:1469491/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:1926581/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:1938793/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:1941749/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:2267362/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:2361824/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:236368/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:2882492/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:3039919/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:2553617/51‑1 (MQ=255) aTTACGAAAATGGATCACCACAAAGCTTTCATCCAGCGACGGGATACCttt < 1:2710751/51‑1 (MQ=255) tACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAg > 1:1745070/1‑69 (MQ=255) tACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAg > 1:1699584/1‑69 (MQ=255) tACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAg > 1:1968742/1‑69 (MQ=255) ggATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACg > 1:3426952/1‑51 (MQ=255) ggATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACg > 1:631433/1‑51 (MQ=255) ggATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACg > 1:1664765/1‑51 (MQ=255) ggATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACg > 1:2264454/1‑51 (MQ=255) ggATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACg > 1:1573320/1‑51 (MQ=255) ggATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACg > 1:2506528/1‑51 (MQ=255) ggATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACg > 1:3183110/1‑51 (MQ=255) cacaAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAAc > 1:1457529/1‑69 (MQ=255) acaAAGCTTTCATCGAGCGACGGGATACCTTTTGCcgcg < 1:2454311/39‑1 (MQ=255) acaAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACggg < 1:2852693/45‑1 (MQ=255) aaGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACgcgc > 1:2250466/1‑69 (MQ=255) aaGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACgcgc > 1:3347956/1‑69 (MQ=255) aaGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACgcgc > 1:1196342/1‑69 (MQ=255) gCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCt > 1:2896754/1‑68 (MQ=255) gCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCt > 1:1648015/1‑68 (MQ=255) gCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCt > 1:2734678/1‑68 (MQ=255) gCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCt > 1:3100843/1‑68 (MQ=255) gCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCt > 1:95371/1‑68 (MQ=255) gCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCt > 1:2783751/1‑68 (MQ=255) cTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACggg < 1:1212459/39‑1 (MQ=255) cGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCTGGAAGTTaa < 1:3152998/69‑1 (MQ=255) cGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCTGGAAGTTaa < 1:155887/69‑1 (MQ=255) cGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCTGGAAGTTaa < 1:922644/69‑1 (MQ=255) cGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCTGGAAGTTaa < 1:114950/69‑1 (MQ=255) | CAGCCAGGGGAAATCGATCCCTTCCAGATTACGAAAATGGATCACCACAAAGCTTTCATCGAGCGACGGGATACCTTTTGCCGCGAACGGGTTTGAAGTGTCGACGGTGGGAACGCGCTGGAAGTTAA > minE/2723147‑2723274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |