| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,726,336 | G→A | 48.9% | Y249Y (TAC→TAT) | yheS ← | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,726,336 | 0 | G | A | 48.9% | ‑2.2 / 39.8 | 47 | Y249Y (TAC→TAT) | yheS | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (3/21); new base A (6/17); total (9/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTA > minE/2726274‑2726392 | gCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATc > 1:1330587/1‑69 (MQ=255)gCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATc > 1:3170867/1‑69 (MQ=255)gCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGGTCCTGCTGGCTTTCGTACATc > 1:1960098/1‑69 (MQ=255) ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGGTTTCATACATCGCTTGTtgct > 1:698749/1‑69 (MQ=255) ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCttgttg > 1:3048808/1‑67 (MQ=255) ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCTTGTtgct > 1:1313723/1‑69 (MQ=255) ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCTTGTtgct > 1:3172843/1‑69 (MQ=255) ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCTTGTtgct > 1:215335/1‑69 (MQ=255) ggAAACGGTCGATATAACTTTGCAAATGCGCCACGCGTTCCTGCTGGCTTTCATACATCGCTTGTtgct > 1:2252193/1‑69 (MQ=255) aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:3050790/68‑1 (MQ=255) aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:954889/68‑1 (MQ=255) aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:336798/68‑1 (MQ=255) aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:3170832/68‑1 (MQ=255) aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:2989531/68‑1 (MQ=255) aGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:2556849/68‑1 (MQ=255) gATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTg < 1:3321919/67‑1 (MQ=255) ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCTCTGTACTTCAAACGAACTGt < 1:2891604/69‑1 (MQ=255) ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:959026/69‑1 (MQ=255) ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:88076/69‑1 (MQ=255) ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:60838/69‑1 (MQ=255) ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:372950/69‑1 (MQ=255) ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:521386/69‑1 (MQ=255) ccTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:369040/69‑1 (MQ=255) ctgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:2018835/69‑1 (MQ=255) tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGt < 1:410559/67‑1 (MQ=255) tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:41464/68‑1 (MQ=255) tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:1514849/68‑1 (MQ=255) tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:1261972/68‑1 (MQ=255) tgctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:2325775/68‑1 (MQ=255) gctgGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTa < 1:1625377/67‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:602231/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:469091/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:513897/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:1614167/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:1357886/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:978163/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:3333083/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:1661036/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2001414/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:3164835/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:3116960/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2232293/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2974180/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2813563/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2246694/36‑1 (MQ=255) tgGCTTTCATACATCGCTTGTTGCTGCGCCAGACgg < 1:2535485/36‑1 (MQ=255) ggCTTTCATACATCGATTGTTGCTGCGCCAGACgg < 1:846806/35‑1 (MQ=37) | GCTTTGGCACGGAAACGGTCGATATAACTTTGCAGATGCGCTACGCGTTCCTGCTGGCTTTCGTACATCGCTTGTTGCTGCGCCAGACGGGTGGCGCGCTGTACTTCAAACGAACTGTA > minE/2726274‑2726392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |