| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,727,579 | C→T | 44.9% | L124L (CTG→TTG) | kefG → | component of potassium effux complex with KefB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,727,579 | 0 | C | T | 44.9% | 5.2 / 29.2 | 40 | L124L (CTG→TTG) | kefG | component of potassium effux complex with KefB |
| Reads supporting (aligned to +/- strand): ref base C (14/8); new base T (11/7); total (25/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGTACTGGCGTAGCGTGATTACCACCGGCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGCCGGATG > minE/2727519‑2727644 | aaGTACTGGCGTAGCGTGATTACCACCGGCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGc > 1:369593/1‑69 (MQ=255) cTGGCGTAGCGTGATTACCACCGGCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTAccc > 1:2114748/1‑69 (MQ=255) cTGGCGTAGCGTGATTACCACCGGCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTAccc > 1:1479309/1‑69 (MQ=255) cTGGCGTAGCGTGATTACCACCGGCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTAccc > 1:52162/1‑69 (MQ=255) cTGGCGTAGCGTGATTACCACCGGCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTAccc > 1:1620398/1‑69 (MQ=255) ggCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGtgctgc > 1:1042597/1‑64 (MQ=255) ggCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGtgctgc > 1:382382/1‑64 (MQ=255) ggCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGtgctgc > 1:27561/1‑64 (MQ=255) ggCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGtgctgc > 1:1594320/1‑64 (MQ=255) ggCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGtgctgc > 1:757725/1‑64 (MQ=255) ggCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGtgctgc > 1:3090434/1‑64 (MQ=255) ggCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGtgctgc > 1:1501375/1‑64 (MQ=255) ggCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGtgctgc > 1:1448994/1‑64 (MQ=255) ggCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGtgctg > 1:399383/1‑63 (MQ=255) tACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCggg < 1:3434295/68‑1 (MQ=255) tACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCggg < 1:273419/68‑1 (MQ=255) tACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCggg < 1:2398729/68‑1 (MQ=255) tACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCggg < 1:1014716/68‑1 (MQ=255) tACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCggg < 1:342899/68‑1 (MQ=255) tACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCggg < 1:2024941/68‑1 (MQ=255) tACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCggg < 1:1705207/68‑1 (MQ=255) tACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCggg < 1:1502727/68‑1 (MQ=255) ggtATGACGCGTTGAATCGCTACCCGATGAGCGATGttct > 1:717221/3‑40 (MQ=37) gTTATGACGCGTTGAATCGCTGCCCGATGAGCGATGttct > 1:2076959/1‑40 (MQ=37) gTTATGACGCGTTGAATCGCTACCCGATGAGCGCTGttct > 1:1927068/1‑40 (MQ=37) gTTATGACGCGTTGAATCGCTACCCGATGAGCGATGttct > 1:359156/1‑40 (MQ=38) gTTATGACGCGTTGAATCGCTACCCGATGAGCGATGttct > 1:644213/1‑40 (MQ=38) gTTATGACGCGTTGAATCGCTACCCGATGAGCGATGttct > 1:2329325/1‑40 (MQ=38) gTTATGACGCGTTGAATCGCTACCCGATGAGCGATGttct > 1:328380/1‑40 (MQ=38) gTTATGACGCGTTGAATCGCTACCCGATGAGCGATGttct > 1:2553647/1‑40 (MQ=38) gTTATGACGCGTTGAATCGCTACCCGATGAGCGATGttct > 1:1508224/1‑40 (MQ=38) gTTATGACGCGTTGAATAGCTACCCGATGAGCGATGttct > 1:2260368/1‑40 (MQ=37) cttATGACGCGTTGAATCGCTACCCGATGATCGATGttct > 1:222226/2‑40 (MQ=25) tATGACGCGTTGAATCGCTACCCGATGAGCGATGTTCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGc < 1:304475/69‑1 (MQ=255) tATGACGCGTTGAATCGCTACCCGATGAGCGATGTTCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGc < 1:512424/69‑1 (MQ=255) tATGACGCGTTGAATCGCTACCCGATGAGCGATGTTCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGc < 1:2095449/69‑1 (MQ=255) tATGACGCGTTGAATCGCTACCCGATGAGCGATGTTCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGc < 1:587873/69‑1 (MQ=255) tATGACGCGTTGAATCGCTACCCGATGAGCGATGTTCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGc < 1:1922109/69‑1 (MQ=255) aTGACGCGTTGAATCGCTACCCGATGAGCGATGTTCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGc < 1:2329075/68‑1 (MQ=255) aTGACGCGTTGAATCGCTACCCGATGAGCGATGTTCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGc < 1:892406/68‑1 (MQ=255) gcgcTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGCCGGATg < 1:1720193/69‑1 (MQ=255) | AAGTACTGGCGTAGCGTGATTACCACCGGCGAGCCGGAAAGTGCTTACCGTTATGACGCGCTGAATCGCTACCCGATGAGCGATGTGCTGCGCCCCTTTGAACTGGCGGCGGGCATGTGCCGGATG > minE/2727519‑2727644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |