| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 236,687 | A→G | 13.2% | S14S (TCA→TCG) | yaiE → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 236,687 | 0 | A | G | 13.2% | 104.7 / 5.6 | 60 | S14S (TCA→TCG) | yaiE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (28/24); new base G (2/6); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
ACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAA > minE/236628‑236747 | aCCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCttt > 1:1989919/1‑69 (MQ=255)aCCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCttt > 1:917440/1‑69 (MQ=255)aCCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCttt > 1:835743/1‑69 (MQ=255)aCCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCttt < 1:81387/69‑1 (MQ=255) cAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCcagc > 1:2448507/1‑69 (MQ=255) cAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCcagc > 1:467015/1‑69 (MQ=255) cAGGCCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCcagc > 1:2134595/1‑69 (MQ=255) aGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCttt < 1:506561/56‑1 (MQ=255) tATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCGATCGGCTTTTCCAGCAGCAGCACTgg > 1:650227/1‑69 (MQ=255) tATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCGATCGGCTTTTCCAGCAGCAGCACTgg > 1:1843872/1‑69 (MQ=255) aTGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCcagcagc > 1:151732/1‑47 (MQ=255) aTGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTg > 1:3229955/1‑68 (MQ=255) aTGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTg > 1:2903828/1‑68 (MQ=255) gAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgt > 1:3280520/1‑69 (MQ=255) gAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgt > 1:1754091/1‑69 (MQ=255) gAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgt > 1:1093168/1‑69 (MQ=255) gAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgt > 1:1953182/1‑69 (MQ=255) gAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgt > 1:940021/1‑69 (MQ=255) aGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgt > 1:2748365/1‑68 (MQ=255) aGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgt > 1:881657/1‑68 (MQ=255) aGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgt > 1:2048043/1‑68 (MQ=255) aGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgt > 1:1921090/1‑68 (MQ=255) tACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgtgt < 1:809951/68‑1 (MQ=255) tACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGtt > 1:453761/1‑69 (MQ=255) tACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGtt > 1:868212/1‑69 (MQ=255) tACTTTTCCGGCAAAGTGAAATCAATCGGCTGTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGgtgt < 1:1290882/68‑1 (MQ=255) cTTTTCCTGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:779633/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:2746213/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:5736/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:1887137/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:3139255/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:2419339/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:3391113/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:454858/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:397191/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:2206004/43‑1 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTAt > 1:1072428/1‑69 (MQ=255) cTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTAt > 1:1927282/1‑69 (MQ=255) cTTTTCC‑GCAAAGTGAAATCAATCGGCTTTTCCagcagcagc < 1:9137/42‑1 (MQ=37) ctgCAAAGTGAAATCGATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTg < 1:1379075/66‑1 (MQ=255) cGGCAAAGTGAAATGGATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTg < 1:368456/68‑1 (MQ=255) cGGCAAAGTGAAATCGATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTg < 1:1112251/68‑1 (MQ=255) cGGCAAAGTGAAATCGATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTg < 1:529136/68‑1 (MQ=255) cGGCAAAGTGAAATCGATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTg < 1:1052921/68‑1 (MQ=255) cGGCAAAGTGAAATCGATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTg < 1:1814808/68‑1 (MQ=255) aaGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCg < 1:1082514/69‑1 (MQ=255) aaGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCg < 1:1361910/69‑1 (MQ=255) aaGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCg < 1:216982/69‑1 (MQ=255) aaGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCg < 1:2862630/69‑1 (MQ=255) gTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa < 1:870319/69‑1 (MQ=255) gTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa < 1:1039683/69‑1 (MQ=255) gTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa < 1:544252/69‑1 (MQ=255) gTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa > 1:3411656/1‑69 (MQ=255) gTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa > 1:1659029/1‑69 (MQ=255) gTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa > 1:2860622/1‑69 (MQ=255) gTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa > 1:250408/1‑69 (MQ=255) gTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa > 1:2131775/1‑69 (MQ=255) gTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGa > 1:1039437/1‑68 (MQ=255) tGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa < 1:3352117/68‑1 (MQ=255) tGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGaa < 1:2634900/68‑1 (MQ=255) | ACCAAACAGGGCCAGTTTATGCTTCAAAGTAATGAGTACTTTTCCGGCAAAGTGAAATCAATCGGCTTTTCCAGCAGCAGCACTGGTCGCGCCAGCGTGGGTGTTATGGTTGAAGGCGAA > minE/236628‑236747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |