| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,727,775 | C→T | 35.3% | S4S (TCC→TCT) | kefB → | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,727,775 | 0 | C | T | 35.3% | 30.3 / 26.0 | 48 | S4S (TCC→TCT) | kefB | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base C (16/15); new base T (12/5); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAGCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATCGC > minE/2727714‑2727839 | agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGGGGCCGCTGATGGAAGGTTCTGAtttt > 1:3000969/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGGGGCCGCTGATGGAAGGTTCTGAtttt > 1:3004792/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAtttt > 1:2603941/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAtttt > 1:2399920/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAtttt > 1:1374712/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAtttt > 1:3187926/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAtttt > 1:54103/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAtttt > 1:517045/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAtttt > 1:1559625/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAtttt > 1:34796/1‑68 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAttt > 1:879343/1‑67 (MQ=255)agagCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGAttt > 1:80468/1‑67 (MQ=255) aCGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGc < 1:301575/69‑1 (MQ=255) aCGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGc < 1:2086452/69‑1 (MQ=255) aCGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGc < 1:2667010/69‑1 (MQ=255) gTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAgg < 1:721126/69‑1 (MQ=255) gTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAgg < 1:2939528/69‑1 (MQ=255) aCTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAg > 1:226756/1‑68 (MQ=255) aCTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGt > 1:1880465/1‑69 (MQ=255) aCTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCG‑‑GATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGc > 1:2201180/1‑69 (MQ=255) tggctggcAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTg < 1:581301/68‑1 (MQ=255) tggctggcAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTg < 1:789750/68‑1 (MQ=255) tggctggcAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTg < 1:279834/68‑1 (MQ=255) tggctggcAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTg < 1:1140586/68‑1 (MQ=255) tggctggcAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTg < 1:1813119/68‑1 (MQ=255) tggctggcAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTg < 1:1408363/68‑1 (MQ=255) tggctggcAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTg < 1:1440563/68‑1 (MQ=255) tggctggcAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTg < 1:2062500/68‑1 (MQ=255) cAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTtct > 1:2258559/1‑69 (MQ=255) cAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTtct > 1:2648025/1‑69 (MQ=255) cAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTtct > 1:3005453/1‑69 (MQ=255) cAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTtct > 1:1374786/1‑69 (MQ=255) aaaTCCGTTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGATTTTTTACTCGCAGGAGTGCTGTTtct < 1:2662815/68‑1 (MQ=255) aaaTCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGATTTTTTACTCGCAGGAGTGCTGTTtct < 1:2169298/68‑1 (MQ=255) aaaTCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGATTTTTTACTCGCAGGAGTGCTGTTtct < 1:1758783/68‑1 (MQ=255) aaaTCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGATTTTTTACTCGCAGGAGTGCTGTTtct < 1:1284417/68‑1 (MQ=255) aaTCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCTGATTTTTTACTCGCAGGAGTGCTGTTtct < 1:3130958/67‑1 (MQ=255) aaTCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTTctct > 1:635361/1‑69 (MQ=255) gCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTg < 1:1171901/54‑1 (MQ=255) ggAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTgg < 1:1610957/68‑1 (MQ=255) gTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATcgc > 1:2088395/1‑69 (MQ=255) gTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATcgc > 1:2552533/1‑69 (MQ=255) gTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATcg > 1:1069343/1‑68 (MQ=255) gTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATcg > 1:520875/1‑68 (MQ=255) gTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATcg > 1:1478586/1‑68 (MQ=255) gTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATcg > 1:3243893/1‑68 (MQ=255) gTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATcg > 1:274526/1‑68 (MQ=255) gTTCCGATTTTTTACTCGCAGGAGTCCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATcgc > 1:3198866/1‑69 (MQ=255) | AGAGCCTACGGTGACTGGCTGGCAAATCCGCTGTCTCCAGGAGGCCGCTGATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCGGTGCCGCTGGCATCGC > minE/2727714‑2727839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |