| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,729,065 | C→T | 60.6% | S434S (AGC→AGT) | kefB → | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,729,065 | 0 | C | T | 60.6% | 29.0 / 47.6 | 71 | S434S (AGC→AGT) | kefB | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base C (14/14); new base T (21/22); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01 | |||||||||||
TGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGCGACGCCACGCAGGTCGATCT > minE/2729008‑2729130 | tGGTCGTTTGCTTATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:3233856/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:826516/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:2796232/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:3177609/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:3312362/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:3338609/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:2498984/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:462335/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:2342363/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:564577/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:592971/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:1875110/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:1815418/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:1788547/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:1242456/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:720213/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:712237/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:708377/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:162753/1‑69 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGCTAAcc > 1:37105/1‑68 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCt < 1:1027335/69‑1 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCt < 1:2971637/69‑1 (MQ=255)tGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCt < 1:2281969/69‑1 (MQ=255)tGGTCGTCTGCTGATGGCAAATAAAATGCGCATTACTGTACTGGAGCGGGATATCAGTGCCGTTAACCt > 1:2623421/1‑69 (MQ=255) cGTTTGCTGATGGCAAATAAAATGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATg > 1:1901494/1‑69 (MQ=255) cGTTTGCTGATGGCAAATAAAATGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATg > 1:1828205/1‑69 (MQ=255) gCTGATGGCAAATAAAATGCGCAGTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCt < 1:813203/60‑1 (MQ=255) tGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGtt < 1:1404410/68‑1 (MQ=255) tGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGtt < 1:696483/68‑1 (MQ=255) tGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGtt < 1:1107964/68‑1 (MQ=255) tGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGtt < 1:2761983/68‑1 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTttat < 1:567915/63‑1 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTttat < 1:571764/63‑1 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTttat < 1:620922/63‑1 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTttat < 1:2696784/63‑1 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTttat < 1:2470963/63‑1 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTttat < 1:1636486/63‑1 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGc > 1:2657112/1‑69 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGc > 1:3418413/1‑69 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGc > 1:290587/1‑69 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGc > 1:689931/1‑69 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGc > 1:2630204/1‑69 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGc > 1:2599993/1‑69 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGc > 1:618785/1‑69 (MQ=255) aCCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGc > 1:2607114/1‑69 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:1831765/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:414944/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:234292/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2122140/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2028824/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2012850/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:1075421/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:1350312/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:1313046/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:1191231/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2690345/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:36783/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2559187/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:3265344/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:3233696/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2623241/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:3079248/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2929021/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2899814/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2871494/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2806432/54‑1 (MQ=255) gAGCGGGATATCAGTGCCGTTAACCTGATGCGCAAATATGGCTACAAAGTttat < 1:2679134/54‑1 (MQ=255) gCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGCGACGCCACGCa > 1:291484/1‑69 (MQ=255) aGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAg > 1:230290/1‑37 (MQ=255) aGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGCGACGCCACGCAGGTCGATCt > 1:3284410/1‑68 (MQ=255) aGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGCGACGCCACGCAGGTCGATCt > 1:1343899/1‑68 (MQ=255) | TGGTCGTTTGCTGATGGCAAATAAAATGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGTAAATACGGCTACAAAGTTTATTACGGCGACGCCACGCAGGTCGATCT > minE/2729008‑2729130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |