| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,732,515 | G→A | 30.3% | L195L (CTG→CTA) | yheO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,732,515 | 0 | G | A | 30.3% | 102.7 / 48.4 | 105 | L195L (CTG→CTA) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (27/46); new base A (8/24); total (35/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
ATCGAAGAAGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAT > minE/2732453‑2732583 | aTCGAAGAAGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAGAATCGTCAGATCGTGCTGAAtctc > 1:1368236/1‑69 (MQ=255)aTCGAAGAAGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAAtctc > 1:1492544/1‑69 (MQ=255)aTCGAAGAAGTGAATGCCGATCGCAATGTTTC‑AATAACGCCAAAAATCGTCAGATCGTGCTGAATctct > 1:2452032/1‑69 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:253441/69‑1 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:20812/69‑1 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1542728/69‑1 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:287923/69‑1 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:3351040/69‑1 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:722556/69‑1 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:742931/69‑1 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1235527/69‑1 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:3213163/69‑1 (MQ=255) gaagaaGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1000747/69‑1 (MQ=255) gaagaaGTGAATGCCGAGCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATctct > 1:672848/1‑67 (MQ=255) caagaagTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1040340/68‑1 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:3136748/1‑69 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:2989160/1‑69 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:3349975/1‑69 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:2544767/1‑69 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:1567844/1‑69 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:568456/1‑69 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:2099495/1‑69 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:678207/1‑69 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:1167294/1‑69 (MQ=255) gTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGaaa > 1:2449568/1‑69 (MQ=255) tGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGAt > 1:1662162/1‑69 (MQ=255) tGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTAAATCTCTAc < 1:1948936/58‑1 (MQ=255) tGCCGATCGCAATGTTTCTAATAACGCCAAAAATAGTCAGATCGTGCTGAATCTCTACGAGAAAggg > 1:693666/1‑67 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:192317/69‑1 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:3212359/69‑1 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:3002509/69‑1 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:3369950/69‑1 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:2733208/69‑1 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:483236/69‑1 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:1477980/69‑1 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:2140306/69‑1 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:1185691/69‑1 (MQ=255) tCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:773553/69‑1 (MQ=255) gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:980828/69‑1 (MQ=255) gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:2779851/69‑1 (MQ=255) gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:345609/69‑1 (MQ=255) gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:2411313/69‑1 (MQ=255) gTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTaaa < 1:1909592/69‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1316907/45‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1479723/45‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:488068/45‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1583629/45‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1683076/45‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1730005/45‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:1939396/45‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:945408/45‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:970390/45‑1 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAg > 1:2581179/1‑51 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:1291483/1‑69 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:1264206/1‑69 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:1635128/1‑69 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:323508/1‑69 (MQ=255) tttCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAg > 1:2057723/1‑69 (MQ=255) tatCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACg < 1:2339908/43‑1 (MQ=255) tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:2229772/68‑1 (MQ=255) tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:6797/68‑1 (MQ=255) tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:503499/68‑1 (MQ=255) tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:3402384/68‑1 (MQ=255) tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:1777454/68‑1 (MQ=255) tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:874940/68‑1 (MQ=255) tCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:2663545/68‑1 (MQ=255) aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2713546/68‑1 (MQ=255) aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:478519/68‑1 (MQ=255) aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2278200/68‑1 (MQ=255) aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2096732/68‑1 (MQ=255) aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:3211013/68‑1 (MQ=255) aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2767352/68‑1 (MQ=255) aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:1963998/68‑1 (MQ=255) aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2781526/68‑1 (MQ=255) aaCGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGa < 1:2336972/62‑1 (MQ=255) aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:580560/1‑51 (MQ=255) aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:960064/1‑51 (MQ=255) aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:2302749/1‑51 (MQ=255) aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:2485283/1‑51 (MQ=255) aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:474335/1‑51 (MQ=255) aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:3224777/1‑51 (MQ=255) aaaTCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTaa > 1:3033872/1‑51 (MQ=255) gTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTg < 1:820149/69‑1 (MQ=255) cAGATCGTGCTGAATCTCTACGAGTAAGGGATCTTCGATATTAAAGa < 1:2692118/47‑1 (MQ=255) aGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGa < 1:3198771/37‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCGACCAGGTTGCTGACCGCCTg < 1:3407222/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1096257/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:727929/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1083846/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1966408/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:2917721/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAGGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1621060/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1302904/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1369039/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:446465/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:3357468/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:881113/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:965772/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1969017/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:201973/69‑1 (MQ=255) | ATCGAAGAAGTGAATGCCGATCGCAATGTTTCTAATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAT > minE/2732453‑2732583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |