| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,732,551 | T→C | 40.4% | D207D (GAT→GAC) | yheO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,732,551 | 0 | T | C | 40.4% | 36.4 / 67.3 | 94 | D207D (GAT→GAC) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (7/49); new base C (29/9); total (36/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.23e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
AATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCC > minE/2732486‑2732612 | aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:478519/68‑1 (MQ=255)aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:1963998/68‑1 (MQ=255)aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2096732/68‑1 (MQ=255)aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2278200/68‑1 (MQ=255)aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:698909/68‑1 (MQ=255)aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2713546/68‑1 (MQ=255)aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2767352/68‑1 (MQ=255)aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:2781526/68‑1 (MQ=255)aataaCGCCAAAAATCGTCAGATCGTGCTAAATCTCTACGAGAAAGGGATCTTCGATATTAAAGACGc < 1:3211013/68‑1 (MQ=255) gTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTg < 1:820149/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCGACCAGGTTGCTGACCGCCTg < 1:3407222/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:2917721/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1893256/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1966408/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1083846/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:727929/69‑1 (MQ=255) gTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTg < 1:1096257/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAGGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1621060/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:446465/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:201973/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1983598/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1969017/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:3357468/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:965772/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:3158694/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1302904/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1369039/69‑1 (MQ=255) gAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:881113/69‑1 (MQ=255) aaTCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACAt < 1:1336710/68‑1 (MQ=255) cGAGAAAGGGATCTTCGATATTAAAGATGCGATCa < 1:504418/35‑1 (MQ=255) cGAGAAAGGGATCTTCGATATTAAAGATGCGATCa < 1:3273364/35‑1 (MQ=255) cGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCaaa < 1:1790455/67‑1 (MQ=255) cGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCaaa < 1:2802495/67‑1 (MQ=255) cGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCaaa < 1:651100/67‑1 (MQ=255) cGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCaaa < 1:2353816/67‑1 (MQ=255) ggATGTTCGATATTAAAGACGCGATCAACCAGGTTGCTGAcc > 1:2848612/1‑42 (MQ=37) ggATCTTCGATATTAAAGACGCGATCAACCATGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:3236168/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGCCCGCCTGAACATCTCCAAACACACTGTCt > 1:821583/1‑69 (MQ=39) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTg > 1:1210933/1‑47 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCa > 1:215551/1‑57 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc > 1:751846/1‑68 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc > 1:2289738/1‑68 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc > 1:1440050/1‑68 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc > 1:2037682/1‑68 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:78832/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:690871/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:3000677/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:691703/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:1993319/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:2866285/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:1507857/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:2817111/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:150597/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:3356860/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:342761/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:650425/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:929463/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:2637571/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:1096521/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:2205845/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:2322305/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:2250233/1‑69 (MQ=255) ggATCTTCGATATTAAAGACGCGATCAACCAGGTTGCTGACCGACTGAACATCTCCAAACACACTGTCt > 1:1804494/1‑69 (MQ=39) ggATCTTCGATATTAAAGACGCGATAAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCt > 1:656832/1‑69 (MQ=255) gATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTa > 1:1136251/1‑69 (MQ=255) gATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTa > 1:2867289/1‑69 (MQ=255) gATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTa > 1:1100777/1‑69 (MQ=255) gATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTa > 1:3403059/1‑69 (MQ=255) gATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTa > 1:2169134/1‑69 (MQ=255) gATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTa > 1:1832825/1‑69 (MQ=255) gATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTATCTa > 1:261343/1‑69 (MQ=255) cGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTAtctc < 1:1320541/67‑1 (MQ=255) atatTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTAtctc < 1:278538/65‑1 (MQ=255) atatTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTAtctc < 1:269694/65‑1 (MQ=255) atatTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTAtctc < 1:948711/65‑1 (MQ=255) tatTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTAtctc < 1:3314040/64‑1 (MQ=255) tatTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTATCTCTACa < 1:1050611/68‑1 (MQ=255) tatTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTATCTCTACa < 1:31791/68‑1 (MQ=255) tatTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTATCTCTACa < 1:3006951/68‑1 (MQ=255) tatTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTATCTCTACa < 1:1077773/68‑1 (MQ=255) tatTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTATCTCTACa < 1:2350956/68‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:2280413/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:978495/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:1019654/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:1416290/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:1449891/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:1576679/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:1752057/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:2233745/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:2650828/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:2874198/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTc < 1:3278620/56‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTATCTCTACATcc < 1:527713/69‑1 (MQ=255) ttAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTATCTCTACATcc < 1:3360617/69‑1 (MQ=255) | AATAACGCCAAAAATCGTCAGATCGTGCTGAATCTCTACGAGAAAGGGATCTTCGATATTAAAGATGCGATCAACCAGGTTGCTGACCGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCC > minE/2732486‑2732612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |