| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,732,742 | T→A | 54.3% | D30E (GAT→GAA) | yheN → | predicted intracellular sulfur oxidation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,732,742 | 0 | T | A | 54.3% | 11.2 / 90.9 | 81 | D30E (GAT→GAA) | yheN | predicted intracellular sulfur oxidation protein |
| Reads supporting (aligned to +/- strand): ref base T (21/16); new base A (32/12); total (53/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
GGGCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCTATAACGCTAACCA > minE/2732677‑2732798 | gggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:1764119/69‑1 (MQ=255)gggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:1679358/69‑1 (MQ=255)gggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:678747/69‑1 (MQ=255)gggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:443551/69‑1 (MQ=255) ggCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGc < 1:1788753/68‑1 (MQ=255) aTACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGa > 1:2459832/1‑66 (MQ=255) aTACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGa > 1:1812264/1‑66 (MQ=255) aTACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAg > 1:3247148/1‑67 (MQ=255) aTACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAg > 1:2398075/1‑67 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:586331/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2450051/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2955477/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2775411/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2693239/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:1733794/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2470664/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2074620/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2100599/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:1147932/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:1827375/69‑1 (MQ=255) gTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca < 1:2186523/69‑1 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCAt > 1:3408190/1‑50 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAagca > 1:96400/1‑60 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:349938/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2444399/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:689858/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2332599/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:847621/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2271545/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:889350/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:1128421/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2156328/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:2150394/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:1662117/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:1056466/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:1937723/1‑69 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGtttt > 1:2469163/1‑67 (MQ=255) aGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTAATAGAAGAAGGCCATGAGTTAAGCAGCGtttttt > 1:896153/1‑69 (MQ=255) cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:1562301/69‑1 (MQ=255) cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:669122/69‑1 (MQ=255) cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:1412962/69‑1 (MQ=255) cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:738306/69‑1 (MQ=255) cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:825245/69‑1 (MQ=255) cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:2059184/69‑1 (MQ=255) cGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:2214592/69‑1 (MQ=255) gAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCt < 1:1738073/68‑1 (MQ=255) tagtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:83268/1‑68 (MQ=255) tagtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:1444800/1‑68 (MQ=255) tagtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:3361876/1‑68 (MQ=255) gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:896478/1‑66 (MQ=255) gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:736403/1‑66 (MQ=255) gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:2961415/1‑66 (MQ=255) gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:604878/1‑66 (MQ=255) gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:473088/1‑66 (MQ=255) gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:1494815/1‑66 (MQ=255) gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:3345374/1‑66 (MQ=255) gtGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATc > 1:1533597/1‑66 (MQ=255) gCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGaa > 1:1998609/1‑69 (MQ=255) cTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAg > 1:1503190/1‑69 (MQ=255) cTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAg > 1:3002264/1‑69 (MQ=255) gTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTc > 1:975685/1‑67 (MQ=255) gTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCt > 1:1521455/1‑68 (MQ=255) tttGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCtat < 1:2814800/69‑1 (MQ=255) tttGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTCTCTATCGGGAAGGGGTCtat < 1:320116/69‑1 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:921910/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:1664600/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:209644/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:2288623/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:1165384/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:1246866/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:698278/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:130830/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:1926366/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:449442/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:406741/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:2156090/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:3286169/1‑42 (MQ=255) tGCGCAGGCGCTAATAGCAGAAGGCCATGAGTTAAGCAGCGttt > 1:2993392/1‑42 (MQ=255) tGCGCAGGCGCTAATAACAGAAGGCCATGAGTTAAGCAGCGttt > 1:1999057/1‑42 (MQ=255) gcTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCTATAACGCTAACCa < 1:903156/69‑1 (MQ=255) gATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCTATaa > 1:2427476/1‑57 (MQ=255) | GGGCCAGCATACGGTACGCAACAGGCGAGTAGTGCTTTTCAGTTTGCGCAGGCGCTGATAGCAGATGGCCATGAGTTAAGCAGCGTCTTTTTCTATCGGGAAGGGGTCTATAACGCTAACCA > minE/2732677‑2732798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |