| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,733,426 | C→T | 32.4% | R7R (CGC→CGT) | yheL → | predicted intracellular sulfur oxidation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,733,426 | 0 | C | T | 32.4% | 114.6 / 66.4 | 129 | R7R (CGC→CGT) | yheL | predicted intracellular sulfur oxidation protein |
| Reads supporting (aligned to +/- strand): ref base C (53/34); new base T (13/29); total (66/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.43e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 129 total aligned reads displayed.
ACTCGCCAACTACGATGTTATTTTGAGGTTTTGAGGCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCTAT > minE/2733365‑2733490 | aCTCGCCAACTACGATGTTATTTTGAGGTTTTGAGGCGTTTATGCTGCACACATTACATCGCTCACCCt < 1:1957137/69‑1 (MQ=255)aCTCGCCAACTACGATGTTATTTTGAGGTTTTGAGGCGTTTATGCTGCACACATTACATCGCTCACCCt < 1:2122311/69‑1 (MQ=255)aCTCGCCAACTACGATGTTATTTTGAGGTTTTGAGGCGTTTATGCTGCACACATTACATCGCTCACCCt < 1:2564181/69‑1 (MQ=255) cTCGCCAACTACGATGTTATTTTGAGGTTTTGAGGCGTTTATGCTGCACACATTACATCGCTCACCCTg > 1:1032150/1‑69 (MQ=255) cTCGCCAACTACGATGTTATTTTGAGGTTTTGAGGCGTTTATGCTGCACACATTACATCGCTCACCCTg > 1:1863228/1‑69 (MQ=255) cTCGCCAACTACGATGTTATTTTGAGGTTTTGAGGCGTTTATGCTGCACACATTACATCGCTCACCCTg > 1:3235872/1‑69 (MQ=255) gttttgaggCGTTTATGCTGCACACATTACATCGCt > 1:851013/1‑36 (MQ=255) gttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCtgctgc > 1:326153/1‑69 (MQ=255) gttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCtgctgc > 1:2223399/1‑69 (MQ=255) gttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCtgctgc > 1:224470/1‑69 (MQ=255) gttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCtgctgc > 1:2519492/1‑69 (MQ=255) gttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCtgctgc < 1:160373/69‑1 (MQ=255) gttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCtgctgc > 1:3353324/1‑69 (MQ=255) gttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCtgctgc > 1:10345/1‑69 (MQ=255) gttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCtgctgc > 1:765698/1‑69 (MQ=255) ttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCg > 1:1936286/1‑69 (MQ=255) ttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCg > 1:127969/1‑69 (MQ=255) ttttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCg > 1:594664/1‑69 (MQ=255) ttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGt > 1:1576856/1‑68 (MQ=255) ttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:1516890/1‑69 (MQ=255) ttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:3403547/1‑69 (MQ=255) ttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:2031195/1‑69 (MQ=255) ttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:61814/1‑69 (MQ=255) ttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:1194723/1‑69 (MQ=255) ttgaggCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGAGTc > 1:897982/1‑69 (MQ=255) ttgaggCATTTATGCTGCACACATTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCg > 1:1388604/1‑67 (MQ=255) ttgaggCATTTATGCTGCACACATTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGt > 1:3090086/1‑68 (MQ=255) ttgaggCATTTATGCTGCACACATTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:575583/1‑69 (MQ=255) ttgaggCATTTATGCTGCACACATTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:2752169/1‑69 (MQ=255) ttgaggCATTTATGCTGCACACATTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:2825165/1‑69 (MQ=255) ttgaggCATTTATGCTGCACACATTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:1030487/1‑69 (MQ=255) ttgaggCATTTATGCTGCACACATTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:1640426/1‑69 (MQ=255) ttgaggCATTTATGCTGCACACATTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:1558352/1‑69 (MQ=255) ttgaggCATTTATGCTGCACACATTACATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTc > 1:1069025/1‑69 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACgg > 1:1541546/1‑41 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa < 1:2277538/42‑1 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa < 1:25709/42‑1 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa < 1:1576175/42‑1 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa > 1:3179773/1‑42 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa < 1:2397883/42‑1 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa < 1:2282037/42‑1 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa > 1:612547/1‑42 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa > 1:1097931/1‑42 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa > 1:765773/1‑42 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa > 1:775647/1‑42 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa < 1:1022477/42‑1 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa < 1:1874921/42‑1 (MQ=255) gTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGa > 1:2014586/1‑42 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACgg > 1:2081476/1‑38 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:601250/69‑1 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa > 1:928163/1‑69 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:1545665/69‑1 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa > 1:3027440/1‑69 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa > 1:3002479/1‑69 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:902650/69‑1 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa > 1:2672917/1‑69 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:2217876/69‑1 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:2347050/69‑1 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:2408634/69‑1 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa > 1:1694204/1‑69 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:1848708/69‑1 (MQ=255) tATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa > 1:1683689/1‑69 (MQ=255) cacaTTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGa < 1:918909/69‑1 (MQ=255) ttACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGa > 1:1945938/1‑65 (MQ=255) ttACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGa > 1:3322393/1‑65 (MQ=255) ttACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGa > 1:763296/1‑65 (MQ=255) aCATCGTTCACCCTGGCTTACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:2850373/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:740272/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:629094/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:108255/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:2257152/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:1246221/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:221791/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:1264219/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:925672/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:366052/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:1302701/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:3374362/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:3325552/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:2345082/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:1485016/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:3146053/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:3144855/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:3123897/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:310859/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:2753626/54‑1 (MQ=255) aCATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:2843561/54‑1 (MQ=255) aCATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCt > 1:2495211/1‑69 (MQ=255) aCATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCt > 1:2017410/1‑69 (MQ=255) aCATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCt > 1:1852837/1‑69 (MQ=255) aCATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCt > 1:1661579/1‑69 (MQ=255) cATCGTTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGa < 1:2883567/53‑1 (MQ=255) tCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCtat < 1:2652893/68‑1 (MQ=255) tCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCtat < 1:2783455/68‑1 (MQ=255) tCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCtat < 1:2159631/68‑1 (MQ=255) tCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCtat < 1:295423/68‑1 (MQ=255) tCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCtat < 1:2532280/68‑1 (MQ=255) tCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCtat < 1:693724/68‑1 (MQ=255) tCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCtat < 1:670640/68‑1 (MQ=255) tCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCtat < 1:575657/68‑1 (MQ=255) | ACTCGCCAACTACGATGTTATTTTGAGGTTTTGAGGCGTTTATGCTGCACACATTACATCGCTCACCCTGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAAGGAGACGAACTGCTAT > minE/2733365‑2733490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |