| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,736,887 | T→C | 52.3% | R677R (CGT→CGC) | fusA → | protein chain elongation factor EF‑G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,736,887 | 0 | T | C | 52.3% | 3.6 / 56.7 | 67 | R677R (CGT→CGC) | fusA | protein chain elongation factor EF‑G |
| Reads supporting (aligned to +/- strand): ref base T (22/10); new base C (17/18); total (39/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CTGAAGTACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCA > minE/2736823‑2736943 | cTGAAGTACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGcat < 1:491358/69‑1 (MQ=255)cTGAAGTACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGcat < 1:3030945/69‑1 (MQ=255)cTGAAGTACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGcat < 1:3011515/69‑1 (MQ=255)cTGAAGTACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGcat < 1:2079230/69‑1 (MQ=255)cTGAAGTACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGcat < 1:2757698/69‑1 (MQ=255)cTGAAGTACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGcat < 1:936856/69‑1 (MQ=255)cTGAAGTACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGcat < 1:1125342/69‑1 (MQ=255) tACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATac > 1:1279034/1‑68 (MQ=255) tACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATac > 1:596009/1‑68 (MQ=255) tACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATac > 1:3220791/1‑68 (MQ=255) tACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATac > 1:1917709/1‑68 (MQ=255) ccGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTg > 1:678769/1‑58 (MQ=255) ccGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACt > 1:2342617/1‑69 (MQ=255) ccGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACt > 1:374751/1‑69 (MQ=255) ccGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACt > 1:922527/1‑69 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:218889/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:2809115/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:971842/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:3123407/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:3127243/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:316290/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:3256118/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:3428245/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:674985/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:951062/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:2188055/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:1264992/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:1263660/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:1216421/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:1782458/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:1890375/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:2039988/68‑1 (MQ=255) aTGTTCGGATATGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGCGCATCATACACTATGGAATTCCt < 1:2064938/68‑1 (MQ=255) cGGATACGCAACTCAGCTGCGTTCTCTAACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGt > 1:3230764/1‑68 (MQ=255) tACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTatgat < 1:1150067/69‑1 (MQ=255) tACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTatgat < 1:235154/69‑1 (MQ=255) tACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTatgat < 1:2393394/69‑1 (MQ=255) cAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCg > 1:2127703/1‑69 (MQ=255) cAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCg > 1:574787/1‑69 (MQ=255) cAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCg > 1:357373/1‑69 (MQ=255) cAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCg > 1:796408/1‑69 (MQ=255) cAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCg > 1:913480/1‑69 (MQ=255) cAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCg > 1:2098259/1‑69 (MQ=255) cAGCTGCGTTCTCTGACCAAAGGTCGTGAATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCg > 1:1863939/1‑69 (MQ=255) ctctGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAg > 1:2717313/1‑44 (MQ=255) ctctGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAg > 1:257060/1‑44 (MQ=255) ctctGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAg > 1:2354252/1‑44 (MQ=255) ctctGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGt > 1:1289831/1‑67 (MQ=255) ctctGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTg > 1:470328/1‑69 (MQ=255) ctctGACCAAAGGTCGTGCATCATACACTATGGAATTACTGAAGTATGATGAAGCGCCGAGTAACGTTg > 1:2052989/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAAc > 1:781303/1‑61 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:12208/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:69639/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:1012744/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:1324203/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:453674/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:419890/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:37624/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:1609719/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:1669607/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:1770420/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:2945023/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:2892791/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:2075095/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:2613146/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:2101623/1‑69 (MQ=255) gACCAAAGGTCGCGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCa > 1:2256460/1‑69 (MQ=255) | CTGAAGTACCGCTGTCTGAAATGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAAGCGCCGAGTAACGTTGCTCA > minE/2736823‑2736943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |