| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,752,749 | C→T | 23.7% | H78H (CAC→CAT) | yhdN → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,752,749 | 0 | C | T | 23.7% | 116.7 / 27.5 | 88 | H78H (CAC→CAT) | yhdN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (30/37); new base T (14/7); total (44/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTACCGCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTG > minE/2752681‑2752816 | ttACCGCCAGAACTT‑AGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCAc < 1:1429856/68‑1 (MQ=255) tACCGCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCAc < 1:2900485/68‑1 (MQ=255) tACCGCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCAc < 1:1697686/68‑1 (MQ=255) tACCGCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCAc < 1:1434404/68‑1 (MQ=255) tACCGCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCAc < 1:2057818/68‑1 (MQ=255) tACCGCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCAc < 1:717787/68‑1 (MQ=255) tACCGCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCAc < 1:923824/68‑1 (MQ=255) tACCGCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCAc < 1:1296818/68‑1 (MQ=255) gCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACgatg < 1:2268912/68‑1 (MQ=255) gCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACgatg < 1:3002091/68‑1 (MQ=255) gCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACgatg < 1:1077669/68‑1 (MQ=255) gCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACgatg < 1:2354495/68‑1 (MQ=255) gCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACgatg < 1:165909/68‑1 (MQ=255) aaCTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGc > 1:872255/1‑69 (MQ=255) aaCTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGc > 1:163144/1‑69 (MQ=255) gCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACg < 1:2754164/40‑1 (MQ=255) gCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACg < 1:1742381/40‑1 (MQ=255) gCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACg < 1:656263/40‑1 (MQ=255) gCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACg < 1:2717862/40‑1 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:908513/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:2413528/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:235993/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:541400/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:3077935/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1936226/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1992499/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:2632540/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1026105/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:809962/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1346501/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1284945/1‑68 (MQ=255) cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:2152790/1‑68 (MQ=255) gCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGt < 1:1542146/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2376723/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1898/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2730092/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2786128/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2149877/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2140919/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2121923/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2065232/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1164670/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1540781/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:3377406/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1661065/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1670909/68‑1 (MQ=255) ttCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCAt < 1:860202/69‑1 (MQ=255) ttCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCAt < 1:1319410/69‑1 (MQ=255) ttCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCAt < 1:2704881/69‑1 (MQ=255) tatTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:3040479/68‑1 (MQ=255) tatTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:263866/68‑1 (MQ=255) tatTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:596420/68‑1 (MQ=255) tatTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:720111/68‑1 (MQ=255) atTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:386279/67‑1 (MQ=255) atTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGaa > 1:2262013/1‑69 (MQ=255) cAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGa < 1:1754042/68‑1 (MQ=255) cAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGa < 1:232183/68‑1 (MQ=255) cAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGa < 1:2664541/68‑1 (MQ=255) cAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGa < 1:1757795/68‑1 (MQ=255) aaGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCaa < 1:229921/54‑2 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTg > 1:1388585/1‑68 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:3056178/1‑69 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:1011867/1‑69 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:835746/1‑69 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:1532831/1‑69 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:261697/1‑69 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGcaca > 1:1618647/1‑66 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:1419220/1‑66 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:1084075/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:603935/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:5630/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:2903909/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:677832/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:2135444/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:3127493/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:2200172/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:2870806/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:298882/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:29337/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:852790/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:504674/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:1306325/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:84311/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:486732/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:1736421/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:3319846/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:2930700/69‑1 (MQ=255) cGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACaa < 1:1350664/69‑1 (MQ=255) cACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGc > 1:2485582/1‑68 (MQ=255) aCGATGATCCTCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:1688017/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:976530/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:558105/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:234329/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:166181/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:1331367/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:1022163/1‑69 (MQ=255) | TTACCGCCAGAACTTGAGCAACGGAGAGAAGCAATTCAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTG > minE/2752681‑2752816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |