| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,752,776 | C→T | 24.8% | S87S (AGC→AGT) | yhdN → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,752,776 | 0 | C | T | 24.8% | 110.7 / 29.1 | 88 | S87S (AGC→AGT) | yhdN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (34/32); new base T (14/8); total (48/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTGAGTACCGATTTTTTACGCG > minE/2752717‑2752840 | cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:2632540/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:2413528/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:235993/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:809962/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:2152790/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1992499/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1936226/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:3077935/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1346501/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1026105/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:541400/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:908513/1‑68 (MQ=255)cAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGAtt > 1:1284945/1‑68 (MQ=255) gCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGt < 1:1542146/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1898/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2786128/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2376723/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1164670/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2149877/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2140919/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2121923/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2065232/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:3377406/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:2730092/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1540781/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1670909/68‑1 (MQ=255) cTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTc < 1:1661065/68‑1 (MQ=255) ttCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCAt < 1:1319410/69‑1 (MQ=255) ttCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCAt < 1:2704881/69‑1 (MQ=255) ttCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCAt < 1:860202/69‑1 (MQ=255) tatTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:263866/68‑1 (MQ=255) tatTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:720111/68‑1 (MQ=255) tatTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:596420/68‑1 (MQ=255) tatTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:3040479/68‑1 (MQ=255) atTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTgg < 1:386279/67‑1 (MQ=255) atTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGaa > 1:2262013/1‑69 (MQ=255) cAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGa < 1:2664541/68‑1 (MQ=255) cAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGa < 1:1757795/68‑1 (MQ=255) cAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGa < 1:232183/68‑1 (MQ=255) cAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGa < 1:1754042/68‑1 (MQ=255) aaGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCaa < 1:229921/54‑2 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTg > 1:1388585/1‑68 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:261697/1‑69 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:1011867/1‑69 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:3056178/1‑69 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:835746/1‑69 (MQ=255) gTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:1532831/1‑69 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGcaca > 1:1618647/1‑66 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGc > 1:1419220/1‑66 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:298882/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:3127493/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:603935/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:5630/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:29337/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:677832/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:2903909/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:2870806/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:1084075/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:2200172/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:2135444/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCg > 1:852790/1‑67 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:2930700/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:504674/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:486732/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:1306325/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:3319846/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:84311/69‑1 (MQ=255) tCCGTCATGATGATCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:1736421/69‑1 (MQ=255) cGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACaa < 1:1350664/69‑1 (MQ=255) cACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGc > 1:2485582/1‑68 (MQ=255) aCGATGATCCTCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:1688017/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:976530/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:558105/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:234329/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:166181/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:1331367/1‑69 (MQ=255) aCGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTg > 1:1022163/1‑69 (MQ=255) atgatCCGCAATATCAAGAGGTTAGTCGTCGATTGTCATTACTGGAATTGAAGCTGCGAc < 1:599835/60‑1 (MQ=255) gatCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTg < 1:573386/69‑1 (MQ=255) gatCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTg < 1:257842/69‑1 (MQ=255) gatCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTg < 1:1434381/69‑1 (MQ=255) ccGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTGAGt > 1:790264/1‑69 (MQ=255) tatCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTTGATTg < 1:1247832/60‑1 (MQ=255) tCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTGAGTACCGAttt > 1:2090811/1‑69 (MQ=255) ggTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTGAGTACCGATTTTTTAcg > 1:3173485/1‑69 (MQ=255) ggTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTGAGTACCGATTTTTTAcg > 1:917893/1‑69 (MQ=255) tAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTGAGTACCGATTTTTTAcgcg > 1:313745/1‑68 (MQ=255) tAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTGAGTACCGATTTTTTAcgcg > 1:2917851/1‑68 (MQ=255) | CAGCTTCTGGATATTCTCAAAGGTATCCGTCACGATGATCCGCAATATCAAGAGGTTAGCCGTCGATTGTCATTACTGGAATTGAAGCTGCGACAAGCTGGATTGAGTACCGATTTTTTACGCG > minE/2752717‑2752840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |