BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,753,807	C→T	71.2%	Q65Q (CAG→CAA)	mscL ←	mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,753,807	0	C	T	71.2%	104.0 / 46.3	104	Q65Q (CAG→CAA)	mscL	mechanosensitive channel
Reads supporting (aligned to +/- strand): ref base C (19/11); new base T (56/18); total (75/29)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 111 total aligned reads displayed.

AATCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGG  >  minE/2753741‑2753875

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:855033/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:295425/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:1505620/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:81552/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:2654564/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:1365017/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:1359374/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:127674/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:789786/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:868615/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:390837/2‑69 (MQ=255)

taTCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCTTg                                                                    >  1:1108069/2‑69 (MQ=255)

       aaaTCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCCTGCGCATcg                                                             >  1:202933/1‑69 (MQ=255)

       aaaTCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCCTGCGCATcg                                                             >  1:771741/1‑69 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:2420274/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:1698139/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:17313/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:1872597/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:1809671/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:1887045/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:1787056/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:2604256/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:2829578/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:3109143/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:1366996/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:1911233/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:2363059/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:791622/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:222999/69‑1 (MQ=255)

                                       aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:926264/69‑1 (MQ=255)

                                        tGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                             <  1:1927730/68‑1 (MQ=255)

                                            tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                        <  1:2709887/69‑1 (MQ=255)

                                            tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                        <  1:2426428/69‑1 (MQ=255)

                                            tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                        <  1:260367/69‑1 (MQ=255)

                                            tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                        <  1:1745426/69‑1 (MQ=255)

                                              acaacaGCAGGGATTTCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATc                                <  1:3248731/59‑1 (MQ=255)

                                              acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATc                                <  1:3226234/59‑1 (MQ=255)

                                              acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                      >  1:176653/1‑69 (MQ=255)

                                              acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                      >  1:630632/1‑69 (MQ=255)

                                              acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                      >  1:1085953/1‑69 (MQ=255)

                                              acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                      >  1:1082016/1‑69 (MQ=255)

                                              acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                      >  1:1012416/1‑69 (MQ=255)

                                              acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                      >  1:2248810/1‑69 (MQ=255)

                                               caacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATc                                <  1:177629/58‑1 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATtaataa              >  1:1438463/1‑65 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATtaataa              >  1:1068595/1‑65 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:453725/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:279107/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:421547/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:2087875/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:1487415/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:2273260/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:1554419/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:1563166/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:1571181/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:1698815/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:1230760/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:845808/1‑66 (MQ=255)

                                                          atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg             >  1:1199113/1‑66 (MQ=255)

                                                          atatCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAg             >  1:143927/1‑66 (MQ=255)

                                                          atatCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAg             >  1:1524146/1‑66 (MQ=255)

                                                          atatCCCCCTGCGCATCGCGTAGCGTGACAGCAAACCGTTTAAAATCGATCCCGCCAATTAATAAg             >  1:273262/1‑66 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGcc                       >  1:610389/1‑55 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGc                        >  1:618140/1‑54 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:3350713/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:1310764/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:1368290/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:617748/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:1178198/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:288174/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:3041500/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:2948851/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:29370/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:2936212/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:292010/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:2809482/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:1655386/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:2604650/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:1778979/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:83458/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:1905614/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:2405635/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:1929944/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:2058074/1‑56 (MQ=255)

                                                           tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                      >  1:2323586/1‑56 (MQ=255)

                                                             tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGa                              <  1:2307930/46‑1 (MQ=255)

                                                             tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGa                              <  1:573636/46‑1 (MQ=255)

                                                             tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGa                              <  1:2332477/46‑1 (MQ=255)

                                                             tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                   >  1:3398657/1‑57 (MQ=255)

                                                             tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                   >  1:339220/1‑57 (MQ=255)

                                                             tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                   >  1:3325720/1‑57 (MQ=255)

                                                             tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                   >  1:2607403/1‑57 (MQ=255)

                                                             tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                   >  1:1787501/1‑57 (MQ=255)

                                                             tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                   >  1:103602/1‑57 (MQ=255)

                                                                  cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg  <  1:369879/69‑1 (MQ=255)

                                                                  cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg  <  1:3111680/69‑1 (MQ=255)

                                                                  cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg  <  1:1887876/69‑1 (MQ=255)

                                                                  cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg  <  1:1784619/69‑1 (MQ=255)

                                                                  cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg  <  1:1306254/69‑1 (MQ=255)

                                                                  cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg  <  1:1200292/69‑1 (MQ=255)

AATCAGAAAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGG  >  minE/2753741‑2753875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`