BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,753,813	A→C	57.2%	D63E (GAT→GAG)	mscL ←	mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,753,813	0	A	C	57.2%	56.9 / 98.8	105	D63E (GAT→GAG)	mscL	mechanosensitive channel
Reads supporting (aligned to +/- strand): ref base A (19/26); new base C (42/18); total (61/44)
Fisher's exact test for biased strand distribution p-value = 5.36e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

Only 100 of 111 total aligned reads displayed.

AAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGAT  >  minE/2753748‑2753881

aaaTCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCCTGCGCATcg                                                                   >  1:202933/1‑69 (MQ=255)

aaaTCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCCTGCGCATcg                                                                   >  1:771741/1‑69 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:2363059/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:1887045/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:1809671/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:1911233/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:1787056/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:17313/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:222999/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:1698139/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:3109143/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:2412593/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:2420274/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:791622/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:2829578/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:1366996/69‑1 (MQ=255)

                                aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:2604256/69‑1 (MQ=255)

                                 tGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                   <  1:1927730/68‑1 (MQ=255)

                                     tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                              <  1:1727264/69‑1 (MQ=255)

                                     tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                              <  1:2426428/69‑1 (MQ=255)

                                     tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                              <  1:260367/69‑1 (MQ=255)

                                     tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                              <  1:2709887/69‑1 (MQ=255)

                                       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATc                                      <  1:3226234/59‑1 (MQ=255)

                                       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                            >  1:2248810/1‑69 (MQ=255)

                                       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                            >  1:1012416/1‑69 (MQ=255)

                                       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                            >  1:176653/1‑69 (MQ=255)

                                       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                            >  1:630632/1‑69 (MQ=255)

                                       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                            >  1:1085953/1‑69 (MQ=255)

                                       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                            >  1:1082016/1‑69 (MQ=255)

                                        caacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATc                                      <  1:177629/58‑1 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATtaataa                    >  1:1438463/1‑65 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATtaataa                    >  1:1068595/1‑65 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:2273260/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:2770214/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:279107/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:845808/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:453725/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:1230760/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:1554419/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:1563166/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:1571181/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:1698815/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:1199113/1‑66 (MQ=255)

                                                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                   >  1:421547/1‑66 (MQ=255)

                                                   atatCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAg                   >  1:1524146/1‑66 (MQ=255)

                                                   atatCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAg                   >  1:1484586/1‑66 (MQ=255)

                                                   atatCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAg                   >  1:143927/1‑66 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTc                                      >  1:2071036/1‑46 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGcc                             >  1:610389/1‑55 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGc                              >  1:618140/1‑54 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:1178198/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:29370/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:2809482/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:83458/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:292010/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:2948851/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:2983074/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:2604650/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:288174/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:1310764/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:1368290/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:3041500/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:2323586/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:1655386/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:2058074/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:1778979/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:1929944/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:617748/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:1905614/1‑56 (MQ=255)

                                                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                            >  1:3350713/1‑56 (MQ=255)

                                                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGa                                    <  1:2307930/46‑1 (MQ=255)

                                                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGa                                    <  1:2332477/46‑1 (MQ=255)

                                                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                         >  1:3398657/1‑57 (MQ=255)

                                                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                         >  1:103602/1‑57 (MQ=255)

                                                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                         >  1:678761/1‑57 (MQ=255)

                                                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                         >  1:1787501/1‑57 (MQ=255)

                                                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                         >  1:339220/1‑57 (MQ=255)

                                                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                         >  1:3325720/1‑57 (MQ=255)

                                                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                         >  1:2607403/1‑57 (MQ=255)

                                                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg        <  1:3111680/69‑1 (MQ=255)

                                                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg        <  1:1200292/69‑1 (MQ=255)

                                                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg        <  1:1887876/69‑1 (MQ=255)

                                                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg        <  1:1306254/69‑1 (MQ=255)

                                                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg        <  1:3399698/69‑1 (MQ=255)

                                                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg        <  1:369879/69‑1 (MQ=255)

                                                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg        <  1:1784619/69‑1 (MQ=255)

                                                            tGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGcc                 <  1:2235129/59‑1 (MQ=255)

                                                            tGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGcc                 <  1:289136/59‑1 (MQ=255)

                                                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa      <  1:1835626/69‑1 (MQ=255)

                                                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa      <  1:499097/69‑1 (MQ=255)

                                                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa      <  1:2353779/69‑1 (MQ=255)

                                                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa      <  1:1862907/69‑1 (MQ=255)

                                                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa      <  1:2968048/69‑1 (MQ=255)

                                                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCACGCCAATTAATAAGCCCAGAGGAGGCa      <  1:3164600/69‑1 (MQ=255)

                                                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCatgat  <  1:2637219/69‑1 (MQ=255)

                                                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCatgat  <  1:1473872/69‑1 (MQ=255)

                                                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCatgat  <  1:2769203/69‑1 (MQ=255)

                                                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCatgat  <  1:1211781/69‑1 (MQ=255)

                                                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCatgat  <  1:2813147/69‑1 (MQ=255)

                                                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCataat  <  1:1363983/69‑1 (MQ=255)

AAATCAAAGACGTTTTGAATGAAGACACCGTAATGCATCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGAT  >  minE/2753748‑2753881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`