BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,753,843	A→G	35.2%	D53D (GAT→GAC)	mscL ←	mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,753,843	0	A	G	35.2%	115.8 / 104.2	170	D53D (GAT→GAC)	mscL	mechanosensitive channel
Reads supporting (aligned to +/- strand): ref base A (63/47); new base G (42/18); total (105/65)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 171 total aligned reads displayed.

ATGCATCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAACCAGTGAAGAGACAA  >  minE/2753780‑2753907

aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                                             <  1:2412593/69‑1 (MQ=255)

aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                                             <  1:926264/69‑1 (MQ=255)

aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                                             <  1:2420274/69‑1 (MQ=255)

aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                                             <  1:1911233/69‑1 (MQ=255)

aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                                             <  1:1887045/69‑1 (MQ=255)

aTGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                                             <  1:17313/69‑1 (MQ=255)

 tGCATCACAACAGCAGGGATATCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGAt                                                             <  1:1927730/68‑1 (MQ=255)

     tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                                                        <  1:260367/69‑1 (MQ=255)

     tCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGc                                                        <  1:1745426/69‑1 (MQ=255)

       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATc                                                                <  1:3226234/59‑1 (MQ=255)

       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                                                      >  1:1012416/1‑69 (MQ=255)

       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                                                      >  1:2248810/1‑69 (MQ=255)

       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                                                      >  1:176653/1‑69 (MQ=255)

       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                                                      >  1:630632/1‑69 (MQ=255)

       acaacaGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCa                                                      >  1:1085953/1‑69 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATtaataa                                              >  1:1438463/1‑65 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:1698815/1‑66 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:2770214/1‑66 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:279107/1‑66 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:126084/1‑66 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:1230760/1‑66 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:453725/1‑66 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:1571181/1‑66 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:1554419/1‑66 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:1487415/1‑66 (MQ=255)

                   atatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCAATTAATAAg                                             >  1:2273260/1‑66 (MQ=255)

                   atatCCCCCTGCGCATCGCGTAGCGTGACAGCAAACCGTTTAAAATCGATCCCGCCAATTAATAAg                                             >  1:273262/1‑66 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTc                                                                >  1:2071036/1‑46 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:2604650/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:2323586/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:83458/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:2058074/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:1178198/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:1905614/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:292010/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:1778979/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:617748/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:1368290/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:29370/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:1655386/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:3041500/1‑56 (MQ=255)

                    tatCCCCTTGGGCCTCGCGTAGCGTGACAGCAAACTGTTTAAAGTCGATCCCGCCa                                                      >  1:3350713/1‑56 (MQ=255)

                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGa                                                              <  1:2332477/46‑1 (MQ=255)

                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                                                   >  1:3398657/1‑57 (MQ=255)

                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                                                   >  1:103602/1‑57 (MQ=255)

                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                                                   >  1:2607403/1‑57 (MQ=255)

                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                                                   >  1:339220/1‑57 (MQ=255)

                      tCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAAtt                                                   >  1:1787501/1‑57 (MQ=255)

                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg                                  <  1:369879/69‑1 (MQ=255)

                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg                                  <  1:3111680/69‑1 (MQ=255)

                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg                                  <  1:1306254/69‑1 (MQ=255)

                           cTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGaggagg                                  <  1:1200292/69‑1 (MQ=255)

                            tGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGcc                                           <  1:2235129/59‑1 (MQ=255)

                            tGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGcc                                           <  1:289136/59‑1 (MQ=255)

                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa                                <  1:2353779/69‑1 (MQ=255)

                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa                                <  1:1862907/69‑1 (MQ=255)

                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa                                <  1:2968048/69‑1 (MQ=255)

                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa                                <  1:499097/69‑1 (MQ=255)

                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCa                                <  1:1835626/69‑1 (MQ=255)

                             gcgcATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCACGCCAATTAATAAGCCCAGAGGAGGCa                                <  1:3164600/69‑1 (MQ=255)

                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCatgat                            <  1:1473872/69‑1 (MQ=255)

                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCatgat                            <  1:2813147/69‑1 (MQ=255)

                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCatgat                            <  1:2637219/69‑1 (MQ=255)

                                 aTCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCataat                            <  1:1363983/69‑1 (MQ=255)

                                   cgcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCAtgatga                          >  1:2252769/1‑69 (MQ=255)

                                   cgcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCAtgatga                          >  1:1482168/1‑69 (MQ=255)

                                   cgcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCAtgatga                          >  1:3389819/1‑69 (MQ=255)

                                   cgcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCAtgatga                          >  1:291153/1‑69 (MQ=255)

                                   cgcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCAtgatga                          >  1:330749/1‑69 (MQ=255)

                                   cgcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCAtgatga                          >  1:3138420/1‑69 (MQ=255)

                                   cgcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCAtgatga                          >  1:1635795/1‑69 (MQ=255)

                                   cgcgTAGCGTGACAGCAAACTGTTTAAAATAGATCCCGCCAATTAATAAGCCCAGAGGAGGCAtgatga                          >  1:2390364/1‑69 (MQ=255)

                                    gcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCatgat                            <  1:3250143/66‑1 (MQ=255)

                                    gcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATgatgat                         >  1:107965/1‑69 (MQ=255)

                                    gcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATgatgat                         >  1:715649/1‑69 (MQ=255)

                                    gcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATgatgat                         >  1:727521/1‑69 (MQ=255)

                                    gcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATgatgat                         >  1:2531474/1‑69 (MQ=255)

                                    gcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATgatgat                         >  1:2627807/1‑69 (MQ=255)

                                    gcgTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATgatgat                         >  1:2876244/1‑69 (MQ=255)

                                      gTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGata                        <  1:1551228/68‑1 (MQ=255)

                                      gTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGata                        <  1:531404/68‑1 (MQ=255)

                                      gTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGata                        <  1:380226/68‑1 (MQ=255)

                                       ttgCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGata                        <  1:1680976/65‑1 (MQ=255)

                                         gCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAg                                             >  1:266307/1‑44 (MQ=255)

                                         gCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCg                     >  1:1414472/1‑68 (MQ=255)

                                         gCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCg                     >  1:2450286/1‑68 (MQ=255)

                                         gCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCg                     >  1:571773/1‑68 (MQ=255)

                                         gCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCg                     >  1:2664084/1‑68 (MQ=255)

                                         gCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCg                     >  1:2762811/1‑68 (MQ=255)

                                         gCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCg                     >  1:3415905/1‑68 (MQ=255)

                                              aCAGCAAACTGTTTAAAATCGATCCCGCCAATtaataa                                              >  1:2995815/1‑38 (MQ=255)

                                              aCAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAAcc               >  1:3009125/1‑69 (MQ=255)

                                              aCAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAAcc               >  1:178553/1‑69 (MQ=255)

                                              aCAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAAcc               >  1:614474/1‑69 (MQ=255)

                                              aCAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAAcc               >  1:2861190/1‑69 (MQ=255)

                                              aCAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAAcc               >  1:1538506/1‑69 (MQ=255)

                                              aCAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAAcc               >  1:2117559/1‑69 (MQ=255)

                                              aCAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAAcc               >  1:1779809/1‑69 (MQ=255)

                                                           tAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAACCAGTGAAGAGACaa  <  1:247376/69‑1 (MQ=255)

                                                            aaaaTCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAACCAGTGAAGAGACaa  <  1:600862/68‑1 (MQ=255)

ATGCATCACAACAGCAGGGATATCCCCCTGCGCATCGCGTAGCGTGACAGCAAACTGTTTAAAATCGATCCCGCCAATTAATAAGCCCAGAGGAGGCATGATGATATCGGCAACCAGTGAAGAGACAA  >  minE/2753780‑2753907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`