| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,755,814 | T→C | 25.3% | P335P (CCA→CCG) | rsmB ← | 16S rRNA m5C967 methyltransferase, S‑adenosyl‑L‑methionine‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,755,814 | 0 | T | C | 25.3% | 130.6 / 41.2 | 110 | P335P (CCA→CCG) | rsmB | 16S rRNA m5C967 methyltransferase, S‑adenosyl‑L‑methionine‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (65/17); new base C (2/26); total (67/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.66e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CGAGAATTTCAGACTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCATCTAATAAAATGCGATCAAACTGT > minE/2755747‑2755877 | cGAGAATTTCAGACTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTg < 1:1146124/69‑1 (MQ=255)cGAGAATTTCAGACTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTg < 1:3391058/69‑1 (MQ=255)cGAGAATTTCAGACTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTg < 1:3037277/69‑1 (MQ=255) gagaATTTCAGACTTCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTgg > 1:2669844/1‑69 (MQ=255) gagaATTTCAGACTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTgg > 1:3057502/1‑69 (MQ=255) gagaATTTCAGACTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTgg > 1:2290474/1‑69 (MQ=255) gagaATTTCAGACTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTgg > 1:1642839/1‑69 (MQ=255) aGACTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGcgacg > 1:1522164/1‑69 (MQ=255) aCTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGcgacga < 1:3043189/68‑1 (MQ=255) cTGCAATTGCGCGGGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAAt < 1:1425324/69‑1 (MQ=255) cTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAAt < 1:2580969/69‑1 (MQ=255) cTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAAt < 1:2589316/69‑1 (MQ=255) cAACTGCGCGAGTTCTGGGATATCGCGATCGCGGCGTAACCATTTAATATCCGGATGGCGACGAATtac > 1:754392/1‑69 (MQ=255) cAACTGCGCGAGTTCTGGGATATCGCGATCGCGGCGTAACCATTTAATATCCGGATGGCGACGAATtac > 1:2794352/1‑69 (MQ=255) ttGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACAcc < 1:1528293/69‑1 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGcgacga > 1:614756/1‑59 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:2937785/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:2942439/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:29340/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:2764723/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:1140806/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:911598/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:3248607/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:2371572/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:3377268/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:1249287/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:2111253/1‑64 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac < 1:1677300/64‑1 (MQ=255) gcgcGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac > 1:69084/1‑64 (MQ=255) cgcgAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATcac < 1:1356046/63‑1 (MQ=255) ttCCGGGAGATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGa < 1:414629/69‑1 (MQ=255) atatCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAgg > 1:1563960/1‑68 (MQ=255) atatCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAgg > 1:2460745/1‑68 (MQ=255) atatCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAgg > 1:1911823/1‑68 (MQ=255) atatCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAgg > 1:3075880/1‑68 (MQ=255) atatCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAgg > 1:2064445/1‑68 (MQ=255) atatCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAg > 1:2977746/1‑67 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGTCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:3234543/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:1302462/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:3210368/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:635749/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:3104930/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:1403579/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:2725387/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:2710712/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:1334663/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:3175598/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:591771/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:2487044/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:2463783/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:557439/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:2355250/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:221221/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcgc > 1:2173801/1‑69 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcg > 1:3154101/1‑68 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcg > 1:489230/1‑68 (MQ=255) atcgcgatcgcgACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGgcg > 1:1835370/1‑68 (MQ=255) atcgcgatcgagACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGATGCTGaa > 1:2513402/1‑61 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:3109047/1‑69 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:3261481/1‑69 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:535218/1‑69 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:394656/1‑69 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:1437067/1‑69 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:2936247/1‑69 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:242978/1‑69 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:1794556/1‑69 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:2764088/1‑69 (MQ=255) tcgcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCa > 1:2517991/1‑69 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:236135/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:2912977/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:3383118/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:2344218/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:2257990/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:919814/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:2095608/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:1934322/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:1699373/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:1472692/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:902406/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:1410597/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:3359953/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:3324526/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:2369190/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:2535010/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:1351504/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:2606884/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:126830/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:2834902/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:1320782/60‑1 (MQ=255) gcgatcgcggCGTAACCATTTAATATCCGGATCGCGACGAATTACACCAGTTGCCGAACa < 1:1659575/60‑1 (MQ=255) gcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCAt > 1:3043446/1‑68 (MQ=255) gcgatcgcgaCGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCAt > 1:867241/1‑68 (MQ=255) gatcgcggCGTAACCATTTAATATCCGGATGGCGACGAATTACACCAGTTGCCGAACa < 1:1368364/58‑1 (MQ=255) atcgcggCGTAACCATTTAATATCCGGATCGCGACGAATTACACCAGTTGCCGAACa < 1:2815473/57‑1 (MQ=255) tAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCATCTAATAAAATGCGATCAAACt > 1:2664407/1‑69 (MQ=255) tAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCATCTAATAAAATGCGATCAAACt > 1:2562281/1‑69 (MQ=255) tAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCATCTAATAAAATGCGATCAAACt > 1:2102809/1‑69 (MQ=255) tatCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCATCTAATAAAATGCGATCAAACTg < 1:915206/67‑1 (MQ=255) tatCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCATCTAATAAAATGCGATCAAACTGt < 1:703389/68‑1 (MQ=255) atCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCATCTAATAAAATGCGATCAAACTGt < 1:964011/67‑1 (MQ=255) | CGAGAATTTCAGACTGCAATTGCGCGAGTTCCGGGATATCGCGATCGCGACGTAACCATTTAATATCTGGATGGCGACGAATCACACCGGTTGCTGAACAAGGCGCATCTAATAAAATGCGATCAAACTGT > minE/2755747‑2755877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |