| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,756,429 | T→A | 54.3% | E130D (GAA→GAT) | rsmB ← | 16S rRNA m5C967 methyltransferase, S‑adenosyl‑L‑methionine‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,756,429 | 0 | T | A | 54.3% | 29.4 / 146.6 | 129 | E130D (GAA→GAT) | rsmB | 16S rRNA m5C967 methyltransferase, S‑adenosyl‑L‑methionine‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (35/24); new base A (17/53); total (52/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
Only 100 of 135 total aligned reads displayed.
GACGCTTCAGCAACCAGGAAGGATGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAGTTGCGGACGCTTAA > minE/2756362‑2756493 | gACGCTTCAGCAACCAGGAAGGATGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACtctt < 1:150433/69‑1 (MQ=255)gACGCTTCAGCAACCAGGAAGGATGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACtctt < 1:1485209/69‑1 (MQ=255)gACGCTTCAGCAACCAGGAAGGATGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACtctt < 1:1204165/69‑1 (MQ=255)gACGCTTCAGCAACCAGGAAGGATGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACtctt < 1:2745200/69‑1 (MQ=255)gACGCTTCAGCAACCAGGAAGGATGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACtctt < 1:470851/69‑1 (MQ=255)gACGCTTCAGCAACCAGGAAGGATGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACtctt < 1:1550506/69‑1 (MQ=255) aggaaggaTGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGa < 1:3235087/69‑1 (MQ=255) aggaaggaTGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGa < 1:3164680/69‑1 (MQ=255) aggaTGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTg > 1:1009924/1‑69 (MQ=255) aggaTGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTg > 1:2346168/1‑69 (MQ=255) aggaTGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTg > 1:84254/1‑69 (MQ=255) aggaTGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTg > 1:1143089/1‑69 (MQ=255) aggaTGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTg > 1:275442/1‑69 (MQ=255) aggaTGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTg > 1:3266784/1‑69 (MQ=255) gCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTa < 1:534359/69‑1 (MQ=255) gCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTa < 1:1433205/69‑1 (MQ=255) gCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTa < 1:1831173/69‑1 (MQ=255) gCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTa < 1:1919500/69‑1 (MQ=255) gCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTa < 1:2132172/69‑1 (MQ=255) cAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTaa > 1:2244445/1‑69 (MQ=255) cAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTaa > 1:3347733/1‑69 (MQ=255) aTAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATac < 1:224446/69‑1 (MQ=255) tAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATaca > 1:384313/1‑69 (MQ=255) tAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATaca < 1:3132318/69‑1 (MQ=255) tAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATaca < 1:13194/69‑1 (MQ=255) tAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATaca > 1:725185/1‑69 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGTAAc < 1:1010585/53‑1 (MQ=39) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:964733/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:2322106/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:900866/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:2414073/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:2491780/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:821098/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:258940/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:2722555/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:7678/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:2874034/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:1193741/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:1725943/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:1552526/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:1348603/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:1360341/53‑1 (MQ=255) gTGCATCACTGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:1905940/53‑1 (MQ=255) gTGCATCACCGGCATTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAAc < 1:1359353/53‑1 (MQ=255) aCTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACGGTTTATc > 1:1387994/1‑67 (MQ=255) aCTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATc > 1:3266021/1‑67 (MQ=255) aCTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCaa > 1:962769/1‑69 (MQ=255) aCTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCaa > 1:1268645/1‑69 (MQ=255) aCTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCaa > 1:2660656/1‑69 (MQ=255) aCTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCa > 1:3291579/1‑68 (MQ=255) aCTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTATATc > 1:3317353/1‑67 (MQ=255) aTTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCAAtccct < 1:202323/68‑3 (MQ=255) aTTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCAAtccct < 1:3213334/68‑3 (MQ=255) aTTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCAAtccct < 1:1294275/68‑3 (MQ=255) aTTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCAAtccct < 1:2122120/68‑3 (MQ=255) aTTAAACTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCAAtccct < 1:3146924/68‑3 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGt < 1:2192675/43‑1 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGt < 1:3111019/43‑1 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGt < 1:3032041/43‑1 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGt < 1:2946089/43‑1 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGt < 1:2472530/43‑1 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGt < 1:2788511/43‑1 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGt < 1:2593002/43‑1 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGt < 1:277698/43‑1 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACAcc > 1:3008934/1‑51 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAg > 1:2900158/1‑69 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAg > 1:2011646/1‑69 (MQ=255) aaaCTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAg > 1:1771175/1‑69 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:2353797/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:2423162/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:341348/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:25012/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:604930/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:782035/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:1516756/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:2744062/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:1183459/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:1108486/53‑1 (MQ=255) aaCTCGGCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGtt < 1:90363/53‑1 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:2175184/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:1133030/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:862744/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:90237/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:2216327/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:596296/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:2936825/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:500621/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:2513501/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:1887824/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:3261252/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:1588931/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:3116577/1‑53 (MQ=255) ggCTAATAACTCATCTTGCTGACGCTGGAACTGACGTAATACACCGTTAATCa > 1:2811800/1‑53 (MQ=255) gCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGt < 1:3018353/36‑1 (MQ=255) aCTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAGTTGCGGACGCTTaa > 1:593708/1‑69 (MQ=255) aCTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAGTTGCGGACGCTTaa > 1:2109202/1‑69 (MQ=255) aCTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAGTTGCGGACGCTTaa > 1:3057908/1‑69 (MQ=255) aCTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAGTTGCGGACGCTTaa > 1:3054266/1‑69 (MQ=255) aCTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAGTTGCGGACGCTTaa > 1:1699742/1‑69 (MQ=255) aCTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAGTTGCGGACGCTTaa > 1:1037296/1‑69 (MQ=255) | GACGCTTCAGCAACCAGGAAGGATGCAGATAACGTGCATCACTGGCATTAAACTCGGCTAATAACTCTTCTTGCTGACGCTGGAACTGGCGTAATACACCGTTTATCAACCCTTTAAGTTGCGGACGCTTAA > minE/2756362‑2756493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |