| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,758,998 | T→C | 34.8% | R178R (CGT→CGC) | smf → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,758,998 | 0 | T | C | 34.8% | 43.8 / 36.4 | 66 | R178R (CGT→CGC) | smf | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (22/21); new base C (3/20); total (25/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.07e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01 | |||||||||||
GCCTTACAGGTAAATGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATG > minE/2758930‑2759065 | gcattACAGGTAAATGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGt < 1:513992/66‑1 (MQ=255)gcCTTACAGGTAAATGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGt < 1:2829285/69‑1 (MQ=255) gTAAATGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGt > 1:101085/1‑60 (MQ=255) gTAAATGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGt > 1:1662518/1‑60 (MQ=255) gTAAATGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGt > 1:1527883/1‑60 (MQ=255) gTAAATGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGt > 1:29192/1‑60 (MQ=255) gTAAATGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGt > 1:1004639/1‑60 (MQ=255) aaTGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTg < 1:3153679/69‑1 (MQ=255) ggggAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTg > 1:1200415/1‑62 (MQ=255) ggggAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTg > 1:203255/1‑62 (MQ=255) ggggAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTg > 1:1847102/1‑62 (MQ=255) ggggAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTg > 1:540318/1‑62 (MQ=255) ggggAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTg < 1:2383181/62‑1 (MQ=255) ggggAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTg > 1:673061/1‑62 (MQ=255) ggggAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTg > 1:2795633/1‑62 (MQ=255) gcggAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTg < 1:3390928/60‑1 (MQ=255) tGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGgcgc < 1:2728480/69‑1 (MQ=255) tGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGgcgc < 1:696860/69‑1 (MQ=255) tGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGgcgc < 1:3125557/69‑1 (MQ=255) ggACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGgcgc < 1:1012495/68‑1 (MQ=255) ggACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGct < 1:2683739/69‑1 (MQ=255) ggACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGct < 1:1615033/69‑1 (MQ=255) gACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGgcgc > 1:2408901/1‑67 (MQ=255) gACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGctc > 1:315968/1‑69 (MQ=255) cTTAATACCATTCATCCCCTCCGCCATGCTCCACTgg < 1:3044264/37‑1 (MQ=21) cTTAATACCATTCATCCCCTCCGCCATGCTCCACTgg < 1:123162/37‑1 (MQ=21) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:2142903/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:1130384/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:912983/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:3336795/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:3047380/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:2964124/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:715094/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:133987/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:1532698/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:1613127/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:356207/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:462623/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:584798/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:2244779/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:2196778/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:466647/37‑1 (MQ=25) cTTAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:2082497/37‑1 (MQ=25) ttAATACCATTCATCCCCGCCGCCATGCTCCACTgg < 1:298125/36‑1 (MQ=37) aCCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCtctcgtctc < 1:269336/68‑1 (MQ=255) aCCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCtctcgtctc < 1:1312304/68‑1 (MQ=255) aCCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCtctcgtctc < 1:3376229/68‑1 (MQ=255) aCCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCtctcgtctc < 1:544718/68‑1 (MQ=255) aCCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCGTGAACAGGGGGGCGCtctcgtctc < 1:950022/68‑1 (MQ=255) ccATTCATCCCCGCCGTCATGCCCGACTTGCTGCCAGTCTGCTTGAACa > 1:1307637/1‑49 (MQ=255) ccATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACa > 1:480995/1‑49 (MQ=255) ccATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACa > 1:1780298/1‑49 (MQ=255) ccATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACa > 1:181912/1‑49 (MQ=255) ccATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACa > 1:3208096/1‑49 (MQ=255) ccATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACa > 1:2739607/1‑49 (MQ=255) ccATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACa > 1:286748/1‑49 (MQ=255) ccATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTAGa > 1:3121955/1‑46 (MQ=255) ccATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGACTGCTTGAACa > 1:2008876/1‑49 (MQ=255) ccATTCATCACCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACa > 1:1555485/1‑49 (MQ=255) acATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACa > 1:50008/2‑49 (MQ=255) cATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACTTGAGCAAGGTGGTGCTCTCGTCTCGGa > 1:83716/1‑69 (MQ=255) cATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACTTGAGCAAGGTGGTGCTCTCGTCTCGGa > 1:2189817/1‑69 (MQ=255) cATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACTTGAGCAAGGTGGTGCTCTCGTCTCGGa > 1:730297/1‑69 (MQ=255) ccccGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTccc > 1:2561787/1‑69 (MQ=255) ccccGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTcc > 1:1231012/1‑68 (MQ=255) ccccGCCGTCATGCCCGACTGGCTGCCAGTCTGATTGAACAGGGGGGCGCTCTCGTCTCGGAATTTccc > 1:3238852/1‑69 (MQ=255) cgccgTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTc < 1:524190/69‑1 (MQ=255) cgccgTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTc < 1:1788934/69‑1 (MQ=255) gTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATg < 1:687735/69‑1 (MQ=255) gTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATg < 1:1292359/69‑1 (MQ=255) gTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATg < 1:2678443/69‑1 (MQ=255) gTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATg < 1:3350974/69‑1 (MQ=255) gTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATg < 1:1026325/69‑1 (MQ=255) | GCCTTACAGGTAAATGGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCTTGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATG > minE/2758930‑2759065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |