| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 240,006 | T→C | 100% | L315L (CTA→CTG) | araJ ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 240,006 | 0 | T | C | 76.4% | 23.0 / 2.4 | 21 | L315L (CTA→CTG) | araJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (5/0); new base C (14/2); total (19/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCTATTTGCCCACCTGCGGCACCTAATAACTCTCCGCCTTTGGCGTTTTGTAGTAACAATATTTGTAGCGGTGCTGAAAGGGCAAATAATCCCGCGCAACAAATAAAAGCAAAAATA > minE/239955‑240072 | cGCTATTTGCCCACCTGCGGCACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAg < 1:1659183/69‑1 (MQ=255)cGCTATTTGCCCACCTGCGGCACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAg < 1:2188125/69‑1 (MQ=255) gCGGCACCTAATAACTCTCCGCCTTTGGCGTTTTGTAGTAACAATATTTGTAGCGGTGCTGAAAGGGCa > 1:3350643/1‑69 (MQ=255) gCGGCACCTAATAACTCTCCGCCTTTGGCGTTTTGTAGTAACAATATTTGTAGCGGTGCTGAAAGGGCa > 1:2836595/1‑69 (MQ=255) gCACCTAATAACTCTCCGCCTTTGGCGTTTTGTAGTAACAATATTTGTAGCGGTGCTGAAAGGGCAaat > 1:1824074/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACaa > 1:2596149/1‑40 (MQ=38) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCGGTGGTGCTGAAAGGGCAaat > 1:2559719/1‑68 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGGAaata > 1:3210773/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaata > 1:2147928/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaata > 1:101816/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaata > 1:1725982/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaata > 1:834407/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaata > 1:1415425/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaata > 1:399514/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaata > 1:3044471/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaata > 1:3306532/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaata > 1:1386159/1‑69 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaat > 1:895045/1‑68 (MQ=255) cACCTAATAACTCTCCGCCTTTGGCATTTTGCAGTAACAATATTTGCAGTGGTGCTGAAAGGGCAaat > 1:2696679/1‑68 (MQ=255) aaCTCTCCGCCTTTGGCGTTTTGTAGTAACAATATTTGTAGCGGTGCTGAAAGGGCAAATAATCCcgc > 1:2865873/1‑68 (MQ=255) aaCTCTCCGCCTTTGGCGTTTTGTAGTAACAATATTTGTAGCGGTGCTGAAAGGGCAAATAATCCcgc > 1:827470/1‑68 (MQ=255) gtagtaACAATATTTGTAGCGGTGCTGAAAGGGCAAATAATCCCGCGCAACAAATAAAAGCAAAAATa < 1:1886126/68‑1 (MQ=255) | CGCTATTTGCCCACCTGCGGCACCTAATAACTCTCCGCCTTTGGCGTTTTGTAGTAACAATATTTGTAGCGGTGCTGAAAGGGCAAATAATCCCGCGCAACAAATAAAAGCAAAAATA > minE/239955‑240072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |