Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,760,838	T→C	65.3%	L77L (TTG→CTG)	yrdC →	dsRNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,760,838	0	T	C	65.3%	66.8 / 49.2	89	L77L (TTG→CTG)	yrdC	dsRNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (18/13);  new base C (36/22);  total (54/35)
Fisher's exact test for biased strand distribution p-value = 8.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAAGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCG  >  minE/2760772‑2760906                                                                   |                                                                     gATATGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtg                                                                    >  1:1489191/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgtctg                                                                >  1:18554/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactg                                                                >  1:1599749/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactg                                                                >  1:2701093/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactg                                                                >  1:2884431/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactg                                                                >  1:909443/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactg                                                                >  1:1603630/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactg                                                                >  1:3369985/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactg                                                                >  1:2407560/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactg                                                                >  1:1175092/1‑69 (MQ=255)     aGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactg                                                                >  1:36580/1‑69 (MQ=255)           tGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGc                                                          >  1:411601/1‑69 (MQ=255)           tGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGc                                                          >  1:2718308/1‑69 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2458767/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:3042222/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2363331/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2492263/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2513726/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2577448/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:218035/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2766474/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2880236/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2922093/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2573513/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:306862/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:3215605/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:3386591/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:363324/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:839470/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2133609/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:2087600/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:1368092/1‑68 (MQ=255)                ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                      >  1:1893689/1‑68 (MQ=255)                ttAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCg                                                      >  1:2850512/1‑68 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:344480/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:1808114/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:1022107/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:2793584/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:2838599/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:2663355/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:998594/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:3414437/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:459331/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:2558602/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:203824/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:2054368/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:2850958/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:2851150/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:1766700/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:1649086/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:2338152/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:161684/68‑1 (MQ=255)                  aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:3079589/68‑1 (MQ=255)                  aaTCGCGGCAAAATACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:2397528/68‑1 (MQ=255)                   aTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:1892762/67‑1 (MQ=255)                            gaTTACGAGCAGCTTAAACCATATATTGATGACACCATGTtgactgac                                                              <  1:261509/47‑1 (MQ=39)                            aaTTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactgac                                                              <  1:1091203/48‑1 (MQ=255)                            aaTTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactgac                                                              <  1:2476458/48‑1 (MQ=255)                            aaTTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactgac                                                              <  1:2465397/48‑1 (MQ=255)                            aaTTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactgac                                                              <  1:2076071/48‑1 (MQ=255)                            aaTTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactgac                                                              <  1:877313/48‑1 (MQ=255)                            aaTTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactgac                                                              <  1:2089970/48‑1 (MQ=255)                            aaTTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactgac                                                              <  1:1577633/48‑1 (MQ=255)                            aaTTACGAGCAGCTTAAACCCTATATTGATGACACCATGTtgactgac                                                              <  1:3105684/48‑1 (MQ=255)                                          tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                           <  1:954562/69‑1 (MQ=255)                                          tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                           <  1:2709055/69‑1 (MQ=255)                                          tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                           <  1:2528885/69‑1 (MQ=255)                                          tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                           >  1:1428708/1‑69 (MQ=255)                                          tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                           >  1:569157/1‑69 (MQ=255)                                          tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                           <  1:2128244/69‑1 (MQ=255)                                            aaCCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                    <  1:1033931/42‑1 (MQ=38)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAGCCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:3206833/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCGGCTGGc                               >  1:1868901/1‑53 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCg                                    >  1:3248645/1‑48 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:2136107/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:1610460/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:81117/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:576067/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:2233065/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:2453457/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:987704/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:408570/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:1085825/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:2849795/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:3395530/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:1687310/1‑69 (MQ=255)                                                      tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:1640715/1‑69 (MQ=255)                                                       gatgaCACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:804050/1‑68 (MQ=255)                                                       gatgaCACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt               >  1:3069449/1‑68 (MQ=255)                                                                   ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAg                            >  1:2730348/1‑43 (MQ=255)                                                                   ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg  >  1:999676/1‑69 (MQ=255)                                                                   ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg  >  1:547975/1‑69 (MQ=255)                                                                   ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg  >  1:3165321/1‑69 (MQ=255)                                                                   ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg  >  1:2709269/1‑69 (MQ=255)                                                                   ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg  >  1:2645545/1‑69 (MQ=255)                                                                   ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg  >  1:1066450/1‑69 (MQ=255)                                                                   ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgc   >  1:2709380/1‑68 (MQ=255)                                                                   |                                                                     GATAAGGGGCTGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCG  >  minE/2760772‑2760906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑