Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,761,700 A→G 100% S172G (AGT→GGT)  aroE → dehydroshikimate reductase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,761,7000AG97.3% 240.3 / ‑3.7 101S172G (AGT→GGT) aroEdehydroshikimate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (64/34);  total (65/36)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 101 total aligned reads displayed.

GAGTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGCAACATCCAGT  >  minE/2761655‑2761756
                                             |                                                        
gagTTGGCTAAATTGTTTGCGGACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:1524609/1‑52 (MQ=38)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:942727/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:1698965/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:2774511/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:2941605/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:2995367/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:1891711/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:3162979/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:1526476/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:1330661/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:3324188/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:1317284/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:3368314/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:3424170/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:1286485/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:2651586/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:729685/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:1216175/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg                                                    >  1:865859/1‑52 (MQ=39)
gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGCTATgg                                                    >  1:112937/1‑52 (MQ=38)
         aaaTTGTTTGCGCCCACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:284806/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2824324/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2372487/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2757560/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2764637/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2580916/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2760528/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2743187/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2723847/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2830843/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2871692/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2930644/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:3094071/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:3112387/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:3129475/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:3250781/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:369236/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:798438/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:887886/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2294398/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:1624233/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:166028/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:1137532/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:1723005/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:1275804/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:1253876/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:1251165/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATAGACGAACTGGAAGGTCAt                                   >  1:84765/1‑60 (MQ=255)
         aaaTTGTTTGCGCACACTGGCAGTATTCACGCGGTGGGTATGGACGAACTGGAAGGTCAt                                   >  1:2758375/1‑60 (MQ=255)
              gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1546006/69‑1 (MQ=255)
              gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1666774/69‑1 (MQ=255)
              gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:3316023/69‑1 (MQ=255)
              gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1786166/69‑1 (MQ=255)
              gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:37001/69‑1 (MQ=255)
              gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:595862/69‑1 (MQ=255)
              gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:2594928/69‑1 (MQ=255)
              gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1109932/69‑1 (MQ=255)
               tttGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:494472/68‑1 (MQ=255)
               tttGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:3277673/68‑1 (MQ=255)
                     cagacTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:251171/4‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGttt                             >  1:3395871/1‑54 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:471363/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:645396/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:1479335/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:1007067/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:317816/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:1942846/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:2244443/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:2671152/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:1002766/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:1546583/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:2971507/1‑68 (MQ=255)
                     cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa               >  1:3070614/1‑68 (MQ=255)
                       cacTGGCAGTATTCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGc            <  1:1545687/69‑1 (MQ=255)
                        acTGGCAGTATTCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGCa           <  1:3091947/69‑1 (MQ=255)
                                  ttCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGCAACATCCAGt  >  1:2549438/1‑68 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:2110401/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1580712/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:2438566/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:880050/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:218604/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:829856/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1214103/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:124466/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:672443/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:2147419/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:629236/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:525569/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1991982/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:3230184/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:353307/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:3420737/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:3420408/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1843238/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:337802/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1781595/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1322535/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1675112/44‑1 (MQ=255)
                                       gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:3240208/44‑1 (MQ=255)
                                       gCGTCGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat                     <  1:1877180/44‑1 (MQ=38)
                                             |                                                        
GAGTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGCAACATCCAGT  >  minE/2761655‑2761756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: