| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,761,700 | A→G | 100% | S172G (AGT→GGT) | aroE → | dehydroshikimate reductase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,761,700 | 0 | A | G | 97.3% | 240.3 / ‑3.7 | 101 | S172G (AGT→GGT) | aroE | dehydroshikimate reductase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (64/34); total (65/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 101 total aligned reads displayed.
GAGTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGCAACATCCAGT > minE/2761655‑2761756 | gagTTGGCTAAATTGTTTGCGGACACTGGCAGTATTCACGCGTTGGGTATgg > 1:1524609/1‑52 (MQ=38)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:942727/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:1698965/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:2774511/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:2941605/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:2995367/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:1891711/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:3162979/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:1526476/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:1330661/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:3324188/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:1317284/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:3368314/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:3424170/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:1286485/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:2651586/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:729685/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:1216175/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATgg > 1:865859/1‑52 (MQ=39)gagTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCACGCGTTGGCTATgg > 1:112937/1‑52 (MQ=38) aaaTTGTTTGCGCCCACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:284806/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2824324/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2372487/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2757560/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2764637/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2580916/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2760528/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2743187/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2723847/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2830843/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2871692/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2930644/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:3094071/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:3112387/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:3129475/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:3250781/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:369236/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:798438/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:887886/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:2294398/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:1624233/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:166028/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:1137532/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:1723005/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:1275804/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:1253876/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCAt > 1:1251165/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATAGACGAACTGGAAGGTCAt > 1:84765/1‑60 (MQ=255) aaaTTGTTTGCGCACACTGGCAGTATTCACGCGGTGGGTATGGACGAACTGGAAGGTCAt > 1:2758375/1‑60 (MQ=255) gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1546006/69‑1 (MQ=255) gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1666774/69‑1 (MQ=255) gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:3316023/69‑1 (MQ=255) gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1786166/69‑1 (MQ=255) gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:37001/69‑1 (MQ=255) gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:595862/69‑1 (MQ=255) gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:2594928/69‑1 (MQ=255) gTTTGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1109932/69‑1 (MQ=255) tttGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:494472/68‑1 (MQ=255) tttGCGCACACTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:3277673/68‑1 (MQ=255) cagacTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:251171/4‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGttt > 1:3395871/1‑54 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:471363/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:645396/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:1479335/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:1007067/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:317816/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:1942846/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:2244443/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:2671152/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:1002766/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:1546583/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:2971507/1‑68 (MQ=255) cacaCTGGCAGTATTCACGCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTaa > 1:3070614/1‑68 (MQ=255) cacTGGCAGTATTCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGc < 1:1545687/69‑1 (MQ=255) acTGGCAGTATTCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGCa < 1:3091947/69‑1 (MQ=255) ttCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGCAACATCCAGt > 1:2549438/1‑68 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:2110401/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1580712/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:2438566/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:880050/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:218604/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:829856/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1214103/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:124466/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:672443/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:2147419/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:629236/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:525569/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1991982/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:3230184/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:353307/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:3420737/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:3420408/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1843238/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:337802/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1781595/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1322535/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1675112/44‑1 (MQ=255) gCGTTGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:3240208/44‑1 (MQ=255) gCGTCGGGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCat < 1:1877180/44‑1 (MQ=38) | GAGTTGGCTAAATTGTTTGCGCACACTGGCAGTATTCAGGCGTTGAGTATGGACGAACTGGAAGGTCATGAGTTTGATCTCATTATTAATGCAACATCCAGT > minE/2761655‑2761756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |