| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,774,792 | G→A | 28.0% | R333R (CGG→CGA) | aceK → | isocitrate dehydrogenase kinase/phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,774,792 | 0 | G | A | 28.0% | 75.8 / 27.9 | 71 | R333R (CGG→CGA) | aceK | isocitrate dehydrogenase kinase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (18/33); new base A (8/12); total (26/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01 | |||||||||||
AGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACG > minE/2774725‑2774850 | aGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCggg < 1:758315/69‑1 (MQ=255)aGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTCATCggg < 1:435687/69‑1 (MQ=255) gTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCggg < 1:1399780/68‑1 (MQ=255) tCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGGTCGGGTa > 1:2757653/1‑68 (MQ=255) tCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTa > 1:843140/1‑68 (MQ=255) cATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTAt > 1:805465/1‑68 (MQ=255) tATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCaaa > 1:1077199/1‑67 (MQ=255) ttCGTGGAATTGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCa < 1:2741893/54‑1 (MQ=255) ttCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCa < 1:2783398/54‑1 (MQ=255) ttCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCa < 1:264874/54‑1 (MQ=255) ttCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCa < 1:2487611/54‑1 (MQ=255) cGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAgg > 1:1508392/1‑69 (MQ=255) cGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAgg > 1:715175/1‑69 (MQ=255) aTGTTGGTGTTTACGTTGCCGGGTTTTGATCGAGTATTCaaa > 1:3115139/1‑42 (MQ=37) aTGTTGGTGTTTACGTTGCCGGGTTTTGATCGAGTATTCaaa > 1:2608842/1‑42 (MQ=37) aTGTTGGTGTTTACGTTGCCGGGTTTTGATCGAGTATTCaaa > 1:2573050/1‑42 (MQ=37) aTGTTGGTGTTTACGTTGCCGGGTTTTGATCGAGTATTCaaa > 1:2184792/1‑42 (MQ=37) aTGTTGGTGTTTACGTTGCCGGGTTTTGATCGAGTATTCaaa > 1:1374660/1‑42 (MQ=37) aTGTTGGTGTTTACGTTGCCGGGTTTTGATCGAGTATTCaaa > 1:623513/1‑42 (MQ=37) aTGTTGGTGTTTACGTTGCC‑‑GTTTTGATCGAGTATTCaaa > 1:3428388/1‑40 (MQ=16) ttGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACa > 1:2148069/1‑52 (MQ=255) ttGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa > 1:583641/1‑67 (MQ=255) ttGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa > 1:1144603/1‑67 (MQ=255) tctgtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAaga < 1:2302573/67‑1 (MQ=255) ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGtc > 1:611450/1‑40 (MQ=255) ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa > 1:3074498/1‑65 (MQ=255) ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAaga < 1:3165856/69‑1 (MQ=255) ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAaga < 1:301741/69‑1 (MQ=255) ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAaga < 1:2486489/69‑1 (MQ=255) ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAaga < 1:47656/69‑1 (MQ=255) ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAaga < 1:3139916/69‑1 (MQ=255) ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAaga < 1:1569928/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:2980429/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:3301801/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:3072006/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:1356062/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:2782321/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:2621477/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:2742467/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:848359/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:818209/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:547320/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:184914/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:548447/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:2318951/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:640481/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:567538/69‑1 (MQ=255) gtTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:2106254/69‑1 (MQ=255) tttACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGAt < 1:2798618/68‑1 (MQ=255) tttACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATg < 1:319115/69‑1 (MQ=255) tttACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATg < 1:2115352/69‑1 (MQ=255) tttACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATg < 1:1279428/69‑1 (MQ=255) tACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAaga < 1:2519885/63‑1 (MQ=255) tACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTc > 1:1919972/1‑69 (MQ=255) tACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTc > 1:65936/1‑69 (MQ=255) tACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTc > 1:773514/1‑69 (MQ=255) tACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTc > 1:2074650/1‑69 (MQ=255) tACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTc > 1:1666487/1‑69 (MQ=255) tACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTc > 1:333965/1‑69 (MQ=255) tACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTc > 1:2814027/1‑69 (MQ=255) cTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAaga < 1:203717/59‑1 (MQ=255) gttttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACg > 1:1587342/3‑69 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:1047356/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:1323385/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:1176225/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:307293/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:9698/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:195750/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:247943/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:2602565/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:1178309/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:3181644/45‑1 (MQ=255) ttGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGa < 1:3037076/45‑1 (MQ=255) ttGATCGAGGATTCAAAGTCATCAAAGACAGGTGCGCGCCGCAGa < 1:1952370/45‑1 (MQ=255) | AGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACG > minE/2774725‑2774850 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |